Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15220 and PS417_15225 are separated by 294 nucleotides PS417_15225 and PS417_15230 are separated by 342 nucleotides
PS417_15220: PS417_15220 - alkane 1-monooxygenase, at 3,336,775 to 3,338,055
_15220
PS417_15225: PS417_15225 - alpha/beta hydrolase, at 3,338,350 to 3,339,273
_15225
PS417_15230: PS417_15230 - 4-hydroxyacetophenone monooxygenase, at 3,339,616 to 3,341,154
_15230
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3337.472 kb on + strand, within PS417_15220 at 3337.472 kb on + strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.598 kb on + strand, within PS417_15220 at 3337.632 kb on + strand, within PS417_15220 at 3337.632 kb on + strand, within PS417_15220 at 3337.633 kb on - strand, within PS417_15220 at 3337.633 kb on - strand at 3337.633 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.809 kb on - strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.910 kb on - strand, within PS417_15220 at 3337.966 kb on + strand at 3337.966 kb on + strand at 3337.967 kb on - strand at 3337.967 kb on - strand at 3338.130 kb on - strand at 3338.161 kb on + strand at 3338.161 kb on + strand at 3338.663 kb on + strand, within PS417_15225 at 3338.663 kb on + strand at 3338.664 kb on - strand, within PS417_15225 at 3338.969 kb on - strand, within PS417_15225 at 3339.109 kb on + strand at 3339.109 kb on + strand, within PS417_15225 at 3339.110 kb on - strand, within PS417_15225 at 3339.128 kb on + strand, within PS417_15225 at 3339.128 kb on + strand, within PS417_15225 at 3339.129 kb on - strand, within PS417_15225 at 3339.129 kb on - strand, within PS417_15225 at 3339.200 kb on + strand at 3339.283 kb on - strand at 3339.283 kb on - strand at 3339.316 kb on + strand at 3339.348 kb on + strand at 3339.349 kb on - strand at 3339.349 kb on - strand at 3339.484 kb on + strand at 3339.485 kb on - strand at 3339.485 kb on - strand at 3339.501 kb on + strand at 3339.507 kb on - strand at 3339.561 kb on - strand at 3339.616 kb on - strand at 3339.616 kb on - strand at 3339.898 kb on + strand, within PS417_15230 at 3339.914 kb on - strand, within PS417_15230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,337,472 + PS417_15220 0.54 +1.6 3,337,472 + PS417_15220 0.54 +0.6 3,337,473 - PS417_15220 0.54 -0.7 3,337,473 - PS417_15220 0.54 +0.7 3,337,473 - PS417_15220 0.54 -0.1 3,337,598 + PS417_15220 0.64 +1.2 3,337,632 + PS417_15220 0.67 +0.5 3,337,632 + PS417_15220 0.67 +0.2 3,337,633 - PS417_15220 0.67 +0.6 3,337,633 - -0.3 3,337,633 - PS417_15220 0.67 -0.4 3,337,647 - PS417_15220 0.68 +0.5 3,337,647 - PS417_15220 0.68 -0.6 3,337,647 - PS417_15220 0.68 +0.5 3,337,809 - PS417_15220 0.81 +0.1 3,337,909 + PS417_15220 0.89 +0.5 3,337,909 + PS417_15220 0.89 +0.0 3,337,909 + PS417_15220 0.89 +0.1 3,337,910 - PS417_15220 0.89 +0.1 3,337,966 + -0.4 3,337,966 + -0.9 3,337,967 - +1.1 3,337,967 - -1.0 3,338,130 - -1.1 3,338,161 + +0.5 3,338,161 + +0.1 3,338,663 + PS417_15225 0.34 -0.9 3,338,663 + -0.9 3,338,664 - PS417_15225 0.34 -1.3 3,338,969 - PS417_15225 0.67 -0.7 3,339,109 + +2.7 3,339,109 + PS417_15225 0.82 -0.7 3,339,110 - PS417_15225 0.82 -2.7 3,339,128 + PS417_15225 0.84 -0.7 3,339,128 + PS417_15225 0.84 -1.1 3,339,129 - PS417_15225 0.84 -1.6 3,339,129 - PS417_15225 0.84 -0.4 3,339,200 + +0.4 3,339,283 - -0.5 3,339,283 - +0.8 3,339,316 + -2.0 3,339,348 + -0.4 3,339,349 - +1.4 3,339,349 - +0.8 3,339,484 + -1.0 3,339,485 - -0.8 3,339,485 - -1.7 3,339,501 + -0.7 3,339,507 - -1.5 3,339,561 - -0.3 3,339,616 - +0.1 3,339,616 - -2.5 3,339,898 + PS417_15230 0.18 +0.1 3,339,914 - PS417_15230 0.19 +1.0
Or see this region's nucleotide sequence