Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14800 and PS417_14805 overlap by 8 nucleotides PS417_14805 and PS417_14810 are separated by 98 nucleotides PS417_14810 and PS417_14815 are separated by 109 nucleotides
PS417_14800: PS417_14800 - diguanylate cyclase, at 3,233,767 to 3,236,349
_14800
PS417_14805: PS417_14805 - AraC family transcriptional regulator, at 3,236,342 to 3,237,163
_14805
PS417_14810: PS417_14810 - Fosmidomycin resistance protein, at 3,237,262 to 3,238,473
_14810
PS417_14815: PS417_14815 - diguanylate cyclase, at 3,238,583 to 3,239,749
_14815
Position (kb)
3237
3238
3239 Strain fitness (log2 ratio)
-1
0
1
2 at 3236.564 kb on + strand, within PS417_14805 at 3236.565 kb on - strand, within PS417_14805 at 3237.176 kb on + strand at 3237.249 kb on - strand at 3237.419 kb on - strand, within PS417_14810 at 3237.973 kb on + strand at 3238.012 kb on + strand, within PS417_14810 at 3238.013 kb on - strand, within PS417_14810 at 3238.237 kb on - strand, within PS417_14810 at 3238.271 kb on - strand, within PS417_14810 at 3238.397 kb on - strand at 3238.540 kb on - strand at 3238.540 kb on - strand at 3238.918 kb on - strand, within PS417_14815 at 3239.063 kb on + strand, within PS417_14815 at 3239.063 kb on + strand, within PS417_14815 at 3239.064 kb on - strand at 3239.264 kb on + strand, within PS417_14815
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,236,564 + PS417_14805 0.27 -1.2 3,236,565 - PS417_14805 0.27 +1.8 3,237,176 + -1.1 3,237,249 - +0.0 3,237,419 - PS417_14810 0.13 -0.4 3,237,973 + +1.5 3,238,012 + PS417_14810 0.62 -0.2 3,238,013 - PS417_14810 0.62 -0.9 3,238,237 - PS417_14810 0.80 +0.2 3,238,271 - PS417_14810 0.83 -1.4 3,238,397 - +0.3 3,238,540 - +0.3 3,238,540 - +0.1 3,238,918 - PS417_14815 0.29 -1.0 3,239,063 + PS417_14815 0.41 +1.1 3,239,063 + PS417_14815 0.41 +0.5 3,239,064 - +0.8 3,239,264 + PS417_14815 0.58 -0.2
Or see this region's nucleotide sequence