Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14645 and PS417_14650 are separated by 162 nucleotides PS417_14650 and PS417_14655 are separated by 127 nucleotides PS417_14655 and PS417_14660 are separated by 60 nucleotides
PS417_14645: PS417_14645 - hypothetical protein, at 3,198,220 to 3,200,418
_14645
PS417_14650: PS417_14650 - chemotaxis protein, at 3,200,581 to 3,202,146
_14650
PS417_14655: PS417_14655 - lysine transporter LysE, at 3,202,274 to 3,202,894
_14655
PS417_14660: PS417_14660 - methyltransferase, at 3,202,955 to 3,203,611
_14660
Position (kb)
3200
3201
3202
3203 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3199.607 kb on + strand, within PS417_14645 at 3199.968 kb on - strand, within PS417_14645 at 3200.328 kb on + strand at 3200.329 kb on - strand at 3200.461 kb on + strand at 3200.461 kb on + strand at 3200.674 kb on + strand at 3200.674 kb on + strand at 3201.046 kb on + strand, within PS417_14650 at 3201.046 kb on + strand, within PS417_14650 at 3201.046 kb on + strand, within PS417_14650 at 3201.232 kb on + strand, within PS417_14650 at 3201.233 kb on - strand, within PS417_14650 at 3201.233 kb on - strand, within PS417_14650 at 3201.233 kb on - strand, within PS417_14650 at 3201.233 kb on - strand, within PS417_14650 at 3202.058 kb on + strand at 3202.059 kb on - strand at 3202.235 kb on - strand at 3202.505 kb on + strand, within PS417_14655 at 3203.040 kb on + strand, within PS417_14660 at 3203.041 kb on - strand, within PS417_14660 at 3203.053 kb on - strand at 3203.124 kb on + strand, within PS417_14660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,199,607 + PS417_14645 0.63 -2.4 3,199,968 - PS417_14645 0.79 +0.6 3,200,328 + -0.2 3,200,329 - +0.9 3,200,461 + +0.4 3,200,461 + +2.0 3,200,674 + +1.3 3,200,674 + -0.5 3,201,046 + PS417_14650 0.30 -0.4 3,201,046 + PS417_14650 0.30 +1.5 3,201,046 + PS417_14650 0.30 +0.3 3,201,232 + PS417_14650 0.42 -1.0 3,201,233 - PS417_14650 0.42 +1.9 3,201,233 - PS417_14650 0.42 +1.6 3,201,233 - PS417_14650 0.42 +0.7 3,201,233 - PS417_14650 0.42 +0.7 3,202,058 + -0.9 3,202,059 - +1.7 3,202,235 - -0.6 3,202,505 + PS417_14655 0.37 -0.4 3,203,040 + PS417_14660 0.13 -0.2 3,203,041 - PS417_14660 0.13 -2.0 3,203,053 - -1.0 3,203,124 + PS417_14660 0.26 -0.8
Or see this region's nucleotide sequence