Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01350 and PS417_01355 are separated by 411 nucleotides PS417_01355 and PS417_01360 are separated by 202 nucleotides PS417_01360 and PS417_01365 are separated by 20 nucleotides
PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681
_01350
PS417_01355: PS417_01355 - membrane protein, at 296,093 to 297,409
_01355
PS417_01360: PS417_01360 - 4-oxalocrotonate tautomerase, at 297,612 to 297,815
_01360
PS417_01365: PS417_01365 - 3-oxoacyl-ACP reductase, at 297,836 to 298,537
_01365
Position (kb) 296
297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 295.140 kb on + strand, within PS417_01350 at 295.314 kb on + strand, within PS417_01350 at 295.314 kb on + strand, within PS417_01350 at 295.315 kb on - strand, within PS417_01350 at 295.315 kb on - strand, within PS417_01350 at 295.345 kb on - strand, within PS417_01350 at 295.507 kb on - strand at 295.603 kb on + strand at 295.603 kb on + strand at 295.604 kb on - strand at 295.785 kb on + strand at 295.788 kb on + strand at 295.789 kb on - strand at 295.843 kb on + strand at 295.844 kb on - strand at 295.844 kb on - strand at 295.855 kb on + strand at 295.900 kb on + strand at 295.901 kb on - strand at 295.984 kb on + strand at 296.084 kb on + strand at 296.084 kb on + strand at 296.085 kb on - strand at 296.086 kb on + strand at 296.087 kb on - strand at 296.087 kb on - strand at 296.087 kb on - strand at 296.222 kb on + strand at 296.223 kb on - strand at 296.223 kb on - strand at 296.223 kb on - strand at 296.237 kb on + strand, within PS417_01355 at 296.238 kb on - strand, within PS417_01355 at 296.349 kb on - strand, within PS417_01355 at 296.525 kb on + strand, within PS417_01355 at 296.525 kb on + strand, within PS417_01355 at 296.561 kb on + strand, within PS417_01355 at 296.562 kb on - strand at 296.562 kb on - strand, within PS417_01355 at 296.591 kb on + strand, within PS417_01355 at 296.592 kb on - strand, within PS417_01355 at 296.592 kb on - strand, within PS417_01355 at 296.592 kb on - strand, within PS417_01355 at 296.714 kb on + strand, within PS417_01355 at 296.715 kb on - strand, within PS417_01355 at 296.726 kb on - strand, within PS417_01355 at 296.726 kb on - strand, within PS417_01355 at 296.762 kb on + strand, within PS417_01355 at 296.799 kb on - strand, within PS417_01355 at 296.799 kb on - strand, within PS417_01355 at 296.799 kb on - strand, within PS417_01355 at 296.814 kb on - strand, within PS417_01355 at 297.086 kb on + strand, within PS417_01355 at 297.176 kb on + strand, within PS417_01355 at 297.177 kb on - strand, within PS417_01355 at 297.189 kb on - strand, within PS417_01355 at 297.218 kb on - strand, within PS417_01355 at 297.266 kb on + strand, within PS417_01355 at 297.267 kb on - strand, within PS417_01355 at 297.273 kb on + strand, within PS417_01355 at 297.273 kb on + strand, within PS417_01355 at 297.274 kb on - strand, within PS417_01355 at 297.274 kb on - strand, within PS417_01355 at 297.350 kb on + strand at 297.351 kb on - strand at 297.407 kb on + strand at 297.408 kb on - strand at 297.408 kb on - strand at 297.469 kb on + strand at 297.518 kb on + strand at 297.518 kb on + strand at 297.519 kb on - strand at 297.555 kb on + strand at 297.555 kb on + strand at 297.555 kb on + strand at 297.651 kb on - strand, within PS417_01360 at 297.771 kb on + strand, within PS417_01360 at 298.100 kb on + strand at 298.154 kb on + strand, within PS417_01365 at 298.154 kb on + strand, within PS417_01365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 295,140 + PS417_01350 0.74 -1.4 295,314 + PS417_01350 0.82 +0.1 295,314 + PS417_01350 0.82 +1.0 295,315 - PS417_01350 0.82 -0.3 295,315 - PS417_01350 0.82 -1.2 295,345 - PS417_01350 0.84 -0.4 295,507 - +0.4 295,603 + +0.2 295,603 + -0.6 295,604 - +0.3 295,785 + -0.9 295,788 + +0.8 295,789 - -0.4 295,843 + +0.8 295,844 - +0.2 295,844 - -0.6 295,855 + -2.9 295,900 + -0.6 295,901 - +1.2 295,984 + +0.3 296,084 + +0.1 296,084 + +1.2 296,085 - +0.5 296,086 + +0.2 296,087 - -1.0 296,087 - -0.2 296,087 - -2.7 296,222 + +0.9 296,223 - -1.8 296,223 - -0.7 296,223 - -0.7 296,237 + PS417_01355 0.11 -0.8 296,238 - PS417_01355 0.11 +1.0 296,349 - PS417_01355 0.19 -1.8 296,525 + PS417_01355 0.33 -1.6 296,525 + PS417_01355 0.33 -1.3 296,561 + PS417_01355 0.36 -0.6 296,562 - -0.6 296,562 - PS417_01355 0.36 -0.6 296,591 + PS417_01355 0.38 -0.5 296,592 - PS417_01355 0.38 -0.0 296,592 - PS417_01355 0.38 -1.4 296,592 - PS417_01355 0.38 -0.5 296,714 + PS417_01355 0.47 +0.3 296,715 - PS417_01355 0.47 +1.8 296,726 - PS417_01355 0.48 -0.8 296,726 - PS417_01355 0.48 +0.1 296,762 + PS417_01355 0.51 +1.3 296,799 - PS417_01355 0.54 -0.8 296,799 - PS417_01355 0.54 +1.7 296,799 - PS417_01355 0.54 -0.2 296,814 - PS417_01355 0.55 -0.7 297,086 + PS417_01355 0.75 -1.2 297,176 + PS417_01355 0.82 -0.4 297,177 - PS417_01355 0.82 +2.8 297,189 - PS417_01355 0.83 -0.7 297,218 - PS417_01355 0.85 -1.5 297,266 + PS417_01355 0.89 -0.1 297,267 - PS417_01355 0.89 -0.2 297,273 + PS417_01355 0.90 +1.1 297,273 + PS417_01355 0.90 -0.0 297,274 - PS417_01355 0.90 -2.6 297,274 - PS417_01355 0.90 -0.2 297,350 + +0.9 297,351 - -0.6 297,407 + -2.0 297,408 - +0.4 297,408 - -0.1 297,469 + +4.1 297,518 + +0.4 297,518 + +0.6 297,519 - +0.1 297,555 + -0.5 297,555 + -2.7 297,555 + -0.1 297,651 - PS417_01360 0.19 +0.8 297,771 + PS417_01360 0.78 -0.0 298,100 + -0.2 298,154 + PS417_01365 0.45 -0.5 298,154 + PS417_01365 0.45 -1.4
Or see this region's nucleotide sequence