Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12870 and PS417_12875 are separated by 5 nucleotides PS417_12875 and PS417_12880 overlap by 33 nucleotides PS417_12880 and PS417_12885 are separated by 176 nucleotides
PS417_12870: PS417_12870 - enoyl-CoA hydratase, at 2,815,186 to 2,815,998
_12870
PS417_12875: PS417_12875 - NADH pyrophosphatase, at 2,816,004 to 2,816,837
_12875
PS417_12880: PS417_12880 - membrane protein, at 2,816,805 to 2,817,584
_12880
PS417_12885: PS417_12885 - phosphatidylcholine synthase, at 2,817,761 to 2,818,480
_12885
Position (kb)
2816
2817 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2815.022 kb on + strand at 2815.022 kb on + strand at 2815.023 kb on - strand at 2815.023 kb on - strand at 2815.023 kb on - strand at 2815.025 kb on - strand at 2815.145 kb on - strand at 2815.145 kb on - strand at 2815.146 kb on + strand at 2815.146 kb on + strand at 2815.146 kb on + strand at 2815.147 kb on - strand at 2815.147 kb on - strand at 2815.147 kb on - strand at 2815.147 kb on - strand at 2815.159 kb on - strand at 2815.702 kb on + strand, within PS417_12870 at 2815.865 kb on - strand, within PS417_12870 at 2815.900 kb on + strand, within PS417_12870 at 2815.923 kb on + strand at 2816.298 kb on + strand, within PS417_12875 at 2816.391 kb on + strand, within PS417_12875 at 2816.392 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.590 kb on + strand, within PS417_12875 at 2816.604 kb on - strand, within PS417_12875 at 2816.610 kb on + strand, within PS417_12875 at 2816.610 kb on + strand, within PS417_12875 at 2816.610 kb on + strand at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.791 kb on - strand at 2817.227 kb on - strand, within PS417_12880 at 2817.564 kb on + strand at 2817.595 kb on + strand at 2817.595 kb on + strand at 2817.596 kb on - strand at 2817.690 kb on + strand at 2817.691 kb on - strand at 2817.806 kb on - strand at 2817.822 kb on + strand at 2817.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,815,022 + -0.2 2,815,022 + +1.7 2,815,023 - -0.0 2,815,023 - -1.7 2,815,023 - +2.5 2,815,025 - -0.6 2,815,145 - +1.3 2,815,145 - +0.2 2,815,146 + +0.2 2,815,146 + -1.7 2,815,146 + -0.7 2,815,147 - -1.8 2,815,147 - +0.7 2,815,147 - -0.1 2,815,147 - -1.1 2,815,159 - -1.2 2,815,702 + PS417_12870 0.63 -1.7 2,815,865 - PS417_12870 0.84 -0.4 2,815,900 + PS417_12870 0.88 +1.1 2,815,923 + -0.8 2,816,298 + PS417_12875 0.35 -0.6 2,816,391 + PS417_12875 0.46 -1.1 2,816,392 - PS417_12875 0.47 +0.0 2,816,509 - PS417_12875 0.61 -1.1 2,816,509 - PS417_12875 0.61 +1.1 2,816,509 - PS417_12875 0.61 +1.4 2,816,509 - PS417_12875 0.61 +0.3 2,816,590 + PS417_12875 0.70 +1.0 2,816,604 - PS417_12875 0.72 +0.3 2,816,610 + PS417_12875 0.73 +0.1 2,816,610 + PS417_12875 0.73 -0.3 2,816,610 + -1.1 2,816,611 - PS417_12875 0.73 -1.5 2,816,611 - PS417_12875 0.73 +0.5 2,816,611 - PS417_12875 0.73 +0.5 2,816,611 - PS417_12875 0.73 +0.6 2,816,791 - +0.4 2,817,227 - PS417_12880 0.54 -1.2 2,817,564 + +0.3 2,817,595 + -0.4 2,817,595 + +0.7 2,817,596 - -0.8 2,817,690 + +0.2 2,817,691 - +0.4 2,817,806 - +0.4 2,817,822 + +0.4 2,817,823 - +0.7
Or see this region's nucleotide sequence