Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12080 and PS417_12085 overlap by 11 nucleotides PS417_12085 and PS417_12090 are separated by 64 nucleotides PS417_12090 and PS417_12095 are separated by 333 nucleotides PS417_12095 and PS417_12100 are separated by 51 nucleotides PS417_12100 and PS417_12105 are separated by 11 nucleotides
PS417_12080: PS417_12080 - membrane protein, at 2,623,762 to 2,625,324
_12080
PS417_12085: PS417_12085 - thioredoxin, at 2,625,314 to 2,625,649
_12085
PS417_12090: PS417_12090 - hypothetical protein, at 2,625,714 to 2,625,962
_12090
PS417_12095: PS417_12095 - ABC transporter substrate-binding protein, at 2,626,296 to 2,627,144
_12095
PS417_12100: PS417_12100 - amino acid ABC transporter permease, at 2,627,196 to 2,627,858
_12100
PS417_12105: PS417_12105 - ABC transporter permease, at 2,627,870 to 2,628,532
_12105
Position (kb)
2626
2627
2628 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2625.342 kb on + strand at 2625.343 kb on - strand at 2625.415 kb on - strand, within PS417_12085 at 2625.501 kb on + strand, within PS417_12085 at 2625.501 kb on + strand, within PS417_12085 at 2625.565 kb on + strand at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.565 kb on + strand, within PS417_12085 at 2625.566 kb on - strand, within PS417_12085 at 2625.642 kb on + strand at 2625.643 kb on - strand at 2625.838 kb on + strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2625.839 kb on - strand, within PS417_12090 at 2626.245 kb on + strand at 2626.319 kb on + strand at 2626.713 kb on + strand, within PS417_12095 at 2626.713 kb on + strand, within PS417_12095 at 2626.831 kb on - strand, within PS417_12095 at 2626.851 kb on + strand, within PS417_12095 at 2626.851 kb on + strand, within PS417_12095 at 2626.852 kb on - strand, within PS417_12095 at 2626.852 kb on - strand, within PS417_12095 at 2626.852 kb on - strand, within PS417_12095 at 2626.912 kb on - strand, within PS417_12095 at 2626.912 kb on - strand, within PS417_12095 at 2626.912 kb on - strand, within PS417_12095 at 2626.974 kb on + strand at 2627.067 kb on + strand at 2627.068 kb on - strand at 2627.099 kb on - strand at 2627.099 kb on - strand at 2627.143 kb on - strand at 2627.185 kb on + strand at 2627.202 kb on + strand at 2627.203 kb on - strand at 2627.373 kb on + strand, within PS417_12100 at 2627.374 kb on - strand, within PS417_12100 at 2627.394 kb on - strand at 2627.424 kb on + strand, within PS417_12100 at 2627.424 kb on + strand, within PS417_12100 at 2627.425 kb on - strand, within PS417_12100 at 2627.505 kb on + strand, within PS417_12100 at 2627.505 kb on + strand at 2627.718 kb on + strand, within PS417_12100 at 2627.718 kb on + strand, within PS417_12100 at 2627.718 kb on + strand, within PS417_12100 at 2627.719 kb on - strand, within PS417_12100 at 2627.721 kb on + strand, within PS417_12100 at 2627.722 kb on - strand, within PS417_12100 at 2627.787 kb on + strand, within PS417_12100 at 2627.873 kb on + strand at 2627.948 kb on + strand, within PS417_12105 at 2627.968 kb on + strand, within PS417_12105 at 2628.015 kb on - strand, within PS417_12105 at 2628.050 kb on + strand, within PS417_12105 at 2628.051 kb on - strand, within PS417_12105 at 2628.084 kb on + strand, within PS417_12105 at 2628.105 kb on - strand, within PS417_12105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,625,342 + +0.1 2,625,343 - -1.2 2,625,415 - PS417_12085 0.30 -0.2 2,625,501 + PS417_12085 0.56 +0.1 2,625,501 + PS417_12085 0.56 -2.4 2,625,565 + +0.1 2,625,565 + PS417_12085 0.75 -0.0 2,625,565 + PS417_12085 0.75 -2.0 2,625,565 + PS417_12085 0.75 +0.6 2,625,565 + PS417_12085 0.75 -0.4 2,625,566 - PS417_12085 0.75 +0.8 2,625,642 + +0.8 2,625,643 - -2.3 2,625,838 + PS417_12090 0.50 -0.0 2,625,839 - PS417_12090 0.50 +1.0 2,625,839 - PS417_12090 0.50 -0.7 2,625,839 - PS417_12090 0.50 -0.4 2,626,245 + -0.7 2,626,319 + -1.6 2,626,713 + PS417_12095 0.49 -2.5 2,626,713 + PS417_12095 0.49 +0.2 2,626,831 - PS417_12095 0.63 +2.9 2,626,851 + PS417_12095 0.65 +0.2 2,626,851 + PS417_12095 0.65 -0.5 2,626,852 - PS417_12095 0.65 -0.1 2,626,852 - PS417_12095 0.65 +2.1 2,626,852 - PS417_12095 0.65 -3.2 2,626,912 - PS417_12095 0.73 -1.0 2,626,912 - PS417_12095 0.73 +0.7 2,626,912 - PS417_12095 0.73 +1.4 2,626,974 + -1.2 2,627,067 + +3.5 2,627,068 - -0.5 2,627,099 - -0.2 2,627,099 - +0.9 2,627,143 - -0.1 2,627,185 + +0.1 2,627,202 + -0.3 2,627,203 - +0.4 2,627,373 + PS417_12100 0.27 -0.0 2,627,374 - PS417_12100 0.27 -0.9 2,627,394 - -0.3 2,627,424 + PS417_12100 0.34 -3.3 2,627,424 + PS417_12100 0.34 +0.4 2,627,425 - PS417_12100 0.35 -0.1 2,627,505 + PS417_12100 0.47 -0.1 2,627,505 + +2.7 2,627,718 + PS417_12100 0.79 -0.1 2,627,718 + PS417_12100 0.79 -0.8 2,627,718 + PS417_12100 0.79 +0.1 2,627,719 - PS417_12100 0.79 +1.7 2,627,721 + PS417_12100 0.79 -0.9 2,627,722 - PS417_12100 0.79 -1.1 2,627,787 + PS417_12100 0.89 -1.9 2,627,873 + -2.0 2,627,948 + PS417_12105 0.12 +0.3 2,627,968 + PS417_12105 0.15 -2.3 2,628,015 - PS417_12105 0.22 -1.6 2,628,050 + PS417_12105 0.27 -0.8 2,628,051 - PS417_12105 0.27 -1.8 2,628,084 + PS417_12105 0.32 -0.2 2,628,105 - PS417_12105 0.35 -0.3
Or see this region's nucleotide sequence