Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11775 and PS417_11780 are separated by 9 nucleotides PS417_11780 and PS417_11785 are separated by 140 nucleotides
PS417_11775: PS417_11775 - acetoin dehydrogenase, at 2,562,380 to 2,563,474
_11775
PS417_11780: PS417_11780 - AraC family transcriptional regulator, at 2,563,484 to 2,564,380
_11780
PS417_11785: PS417_11785 - glutamine synthetase, at 2,564,521 to 2,565,879
_11785
Position (kb)
2563
2564
2565 Strain fitness (log2 ratio)
-1
0
1
2 at 2562.726 kb on + strand, within PS417_11775 at 2562.958 kb on - strand, within PS417_11775 at 2563.097 kb on + strand, within PS417_11775 at 2563.098 kb on - strand, within PS417_11775 at 2563.452 kb on + strand at 2563.472 kb on + strand at 2563.536 kb on + strand at 2563.536 kb on + strand at 2563.845 kb on - strand, within PS417_11780 at 2563.845 kb on - strand, within PS417_11780 at 2564.079 kb on + strand, within PS417_11780 at 2564.127 kb on + strand, within PS417_11780 at 2564.127 kb on + strand, within PS417_11780 at 2564.128 kb on - strand, within PS417_11780 at 2564.128 kb on - strand, within PS417_11780 at 2564.220 kb on + strand, within PS417_11780 at 2564.400 kb on + strand at 2564.401 kb on - strand at 2564.431 kb on + strand at 2564.431 kb on + strand at 2564.432 kb on - strand at 2564.432 kb on - strand at 2564.722 kb on + strand, within PS417_11785 at 2564.723 kb on - strand, within PS417_11785 at 2564.723 kb on - strand, within PS417_11785 at 2564.725 kb on + strand, within PS417_11785 at 2564.726 kb on - strand at 2564.726 kb on - strand, within PS417_11785 at 2564.785 kb on + strand, within PS417_11785 at 2564.786 kb on - strand, within PS417_11785 at 2564.950 kb on + strand, within PS417_11785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,562,726 + PS417_11775 0.32 +0.1 2,562,958 - PS417_11775 0.53 -0.2 2,563,097 + PS417_11775 0.65 -0.0 2,563,098 - PS417_11775 0.66 -0.1 2,563,452 + +0.3 2,563,472 + -0.1 2,563,536 + -0.3 2,563,536 + -0.8 2,563,845 - PS417_11780 0.40 +0.0 2,563,845 - PS417_11780 0.40 +0.7 2,564,079 + PS417_11780 0.66 +0.1 2,564,127 + PS417_11780 0.72 +2.0 2,564,127 + PS417_11780 0.72 +0.4 2,564,128 - PS417_11780 0.72 +0.2 2,564,128 - PS417_11780 0.72 +0.2 2,564,220 + PS417_11780 0.82 +2.7 2,564,400 + +0.6 2,564,401 - +0.2 2,564,431 + +2.7 2,564,431 + +0.3 2,564,432 - -0.0 2,564,432 - -1.0 2,564,722 + PS417_11785 0.15 +0.9 2,564,723 - PS417_11785 0.15 +1.0 2,564,723 - PS417_11785 0.15 +0.1 2,564,725 + PS417_11785 0.15 -0.4 2,564,726 - +0.5 2,564,726 - PS417_11785 0.15 -0.9 2,564,785 + PS417_11785 0.19 +0.6 2,564,786 - PS417_11785 0.19 +0.4 2,564,950 + PS417_11785 0.32 -0.1
Or see this region's nucleotide sequence