Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11760 and PS417_11765 are separated by 309 nucleotides PS417_11765 and PS417_11770 are separated by 26 nucleotides
PS417_11760: PS417_11760 - Fis family transcriptional regulator, at 2,558,185 to 2,560,050
_11760
PS417_11765: PS417_11765 - ABC transporter substrate-binding protein, at 2,560,360 to 2,561,337
_11765
PS417_11770: PS417_11770 - pyruvate dehydrogenase, at 2,561,364 to 2,562,383
_11770
Position (kb)
2560
2561
2562 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2559.690 kb on - strand, within PS417_11760 at 2560.051 kb on - strand at 2560.079 kb on + strand at 2560.079 kb on + strand at 2560.092 kb on - strand at 2560.307 kb on + strand at 2560.308 kb on - strand at 2560.308 kb on - strand at 2560.399 kb on + strand at 2560.400 kb on - strand at 2560.621 kb on + strand, within PS417_11765 at 2560.622 kb on - strand, within PS417_11765 at 2560.641 kb on - strand, within PS417_11765 at 2560.892 kb on - strand, within PS417_11765 at 2561.075 kb on + strand, within PS417_11765 at 2561.076 kb on - strand, within PS417_11765 at 2561.116 kb on + strand, within PS417_11765 at 2561.320 kb on + strand at 2561.339 kb on - strand at 2561.347 kb on + strand at 2561.347 kb on + strand at 2561.382 kb on + strand at 2561.444 kb on + strand at 2561.922 kb on + strand, within PS417_11770 at 2561.922 kb on + strand, within PS417_11770 at 2561.955 kb on + strand, within PS417_11770 at 2561.955 kb on + strand at 2562.315 kb on + strand at 2562.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,559,690 - PS417_11760 0.81 -0.8 2,560,051 - -1.3 2,560,079 + +0.3 2,560,079 + +0.3 2,560,092 - +3.1 2,560,307 + +1.0 2,560,308 - -0.4 2,560,308 - -3.2 2,560,399 + +0.2 2,560,400 - -0.2 2,560,621 + PS417_11765 0.27 -0.0 2,560,622 - PS417_11765 0.27 +0.2 2,560,641 - PS417_11765 0.29 -0.7 2,560,892 - PS417_11765 0.54 +1.1 2,561,075 + PS417_11765 0.73 +0.3 2,561,076 - PS417_11765 0.73 +2.0 2,561,116 + PS417_11765 0.77 +0.2 2,561,320 + -0.1 2,561,339 - -0.3 2,561,347 + -0.6 2,561,347 + +0.1 2,561,382 + +0.1 2,561,444 + -0.6 2,561,922 + PS417_11770 0.55 -0.9 2,561,922 + PS417_11770 0.55 +1.3 2,561,955 + PS417_11770 0.58 +1.5 2,561,955 + +0.1 2,562,315 + -0.9 2,562,316 - -1.8
Or see this region's nucleotide sequence