Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11730 and PS417_11735 are separated by 146 nucleotides PS417_11735 and PS417_11740 are separated by 123 nucleotides PS417_11740 and PS417_11745 are separated by 182 nucleotides
PS417_11730: PS417_11730 - PvdJ/PvdD/PvdP-like protein, at 2,550,764 to 2,552,398
_11730
PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780
_11735
PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374
_11740
PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994
_11745
Position (kb)
2552
2553
2554 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2551.785 kb on - strand, within PS417_11730 at 2551.988 kb on + strand, within PS417_11730 at 2552.377 kb on - strand at 2552.702 kb on + strand, within PS417_11735 at 2552.702 kb on + strand, within PS417_11735 at 2553.143 kb on + strand, within PS417_11735 at 2553.200 kb on + strand, within PS417_11735 at 2553.213 kb on - strand, within PS417_11735 at 2553.597 kb on + strand, within PS417_11735 at 2553.603 kb on + strand, within PS417_11735 at 2553.605 kb on + strand at 2553.606 kb on - strand, within PS417_11735 at 2553.606 kb on - strand, within PS417_11735 at 2553.683 kb on + strand at 2553.684 kb on - strand at 2553.772 kb on - strand at 2553.941 kb on + strand at 2554.315 kb on + strand, within PS417_11740 at 2554.330 kb on + strand at 2554.764 kb on + strand, within PS417_11745 at 2554.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,551,785 - PS417_11730 0.62 -0.1 2,551,988 + PS417_11730 0.75 -0.0 2,552,377 - -1.5 2,552,702 + PS417_11735 0.13 +0.4 2,552,702 + PS417_11735 0.13 +0.0 2,553,143 + PS417_11735 0.48 +1.1 2,553,200 + PS417_11735 0.53 +0.7 2,553,213 - PS417_11735 0.54 -1.6 2,553,597 + PS417_11735 0.85 +1.1 2,553,603 + PS417_11735 0.86 +0.3 2,553,605 + +1.4 2,553,606 - PS417_11735 0.86 +2.4 2,553,606 - PS417_11735 0.86 -0.1 2,553,683 + +1.2 2,553,684 - +0.1 2,553,772 - +1.8 2,553,941 + -2.4 2,554,315 + PS417_11740 0.87 -0.8 2,554,330 + +0.2 2,554,764 + PS417_11745 0.47 -1.0 2,554,764 + +4.5
Or see this region's nucleotide sequence