Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11265 and PS417_11270 overlap by 14 nucleotides PS417_11270 and PS417_11275 are separated by 148 nucleotides PS417_11275 and PS417_11280 are separated by 8 nucleotides PS417_11280 and PS417_11285 overlap by 4 nucleotides
PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426
_11265
PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778
_11270
PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376
_11275
PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254
_11280
PS417_11285: PS417_11285 - general secretion pathway protein GspL, at 2,415,251 to 2,416,312
_11285
Position (kb)
2414
2415
2416 Strain fitness (log2 ratio)
-1
0
1 at 2413.748 kb on + strand at 2413.774 kb on + strand at 2413.775 kb on - strand at 2413.863 kb on + strand at 2414.129 kb on - strand, within PS417_11275 at 2414.269 kb on + strand, within PS417_11275 at 2414.269 kb on + strand at 2414.345 kb on + strand at 2414.345 kb on + strand at 2414.375 kb on - strand at 2414.770 kb on + strand at 2414.771 kb on - strand, within PS417_11280 at 2414.771 kb on - strand, within PS417_11280 at 2414.919 kb on + strand, within PS417_11280 at 2414.920 kb on - strand, within PS417_11280 at 2414.942 kb on + strand, within PS417_11280 at 2414.943 kb on - strand, within PS417_11280 at 2414.943 kb on - strand, within PS417_11280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,413,748 + -1.6 2,413,774 + -1.7 2,413,775 - +0.8 2,413,863 + -1.0 2,414,129 - PS417_11275 0.45 -0.7 2,414,269 + PS417_11275 0.76 -0.9 2,414,269 + +0.1 2,414,345 + +0.5 2,414,345 + -0.4 2,414,375 - +0.5 2,414,770 + +0.1 2,414,771 - PS417_11280 0.44 +0.6 2,414,771 - PS417_11280 0.44 +0.2 2,414,919 + PS417_11280 0.61 +0.9 2,414,920 - PS417_11280 0.61 +0.4 2,414,942 + PS417_11280 0.64 +1.0 2,414,943 - PS417_11280 0.64 -0.5 2,414,943 - PS417_11280 0.64 -0.7
Or see this region's nucleotide sequence