Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10575 and PS417_10580 are separated by 18 nucleotides PS417_10580 and PS417_10585 are separated by 11 nucleotides PS417_10585 and PS417_10590 are separated by 153 nucleotides
PS417_10575: PS417_10575 - DNA alkylation repair protein, at 2,258,388 to 2,259,488
_10575
PS417_10580: PS417_10580 - glutamine synthetase, at 2,259,507 to 2,260,853
_10580
PS417_10585: PS417_10585 - Cro/Cl family transcriptional regulator, at 2,260,865 to 2,261,422
_10585
PS417_10590: PS417_10590 - hypothetical protein, at 2,261,576 to 2,261,884
_10590
Position (kb)
2259
2260
2261 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2258.593 kb on + strand, within PS417_10575 at 2258.915 kb on - strand, within PS417_10575 at 2259.260 kb on - strand, within PS417_10575 at 2259.509 kb on - strand at 2260.039 kb on + strand, within PS417_10580 at 2260.039 kb on + strand, within PS417_10580 at 2260.040 kb on - strand, within PS417_10580 at 2260.084 kb on + strand at 2260.085 kb on - strand, within PS417_10580 at 2260.244 kb on + strand, within PS417_10580 at 2260.245 kb on - strand, within PS417_10580 at 2260.319 kb on + strand, within PS417_10580 at 2260.408 kb on + strand, within PS417_10580 at 2260.627 kb on + strand at 2260.783 kb on + strand at 2260.866 kb on + strand at 2260.866 kb on + strand at 2260.867 kb on - strand at 2260.873 kb on - strand at 2261.154 kb on + strand, within PS417_10585 at 2261.155 kb on - strand, within PS417_10585 at 2261.218 kb on - strand, within PS417_10585 at 2261.223 kb on + strand, within PS417_10585 at 2261.224 kb on - strand, within PS417_10585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,258,593 + PS417_10575 0.19 -0.2 2,258,915 - PS417_10575 0.48 -0.1 2,259,260 - PS417_10575 0.79 +0.2 2,259,509 - +0.9 2,260,039 + PS417_10580 0.39 +0.1 2,260,039 + PS417_10580 0.39 -1.1 2,260,040 - PS417_10580 0.40 -1.1 2,260,084 + -0.9 2,260,085 - PS417_10580 0.43 -2.8 2,260,244 + PS417_10580 0.55 -0.4 2,260,245 - PS417_10580 0.55 -0.7 2,260,319 + PS417_10580 0.60 +0.8 2,260,408 + PS417_10580 0.67 -1.0 2,260,627 + -0.7 2,260,783 + -0.3 2,260,866 + -1.8 2,260,866 + +0.1 2,260,867 - -2.5 2,260,873 - -0.3 2,261,154 + PS417_10585 0.52 +0.2 2,261,155 - PS417_10585 0.52 -0.0 2,261,218 - PS417_10585 0.63 -0.7 2,261,223 + PS417_10585 0.64 -0.5 2,261,224 - PS417_10585 0.64 +0.1
Or see this region's nucleotide sequence