Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10460 and PS417_10465 overlap by 1 nucleotides PS417_10465 and PS417_10470 are separated by 110 nucleotides
PS417_10460: PS417_10460 - selenide, water dikinase, at 2,233,412 to 2,234,446
_10460
PS417_10465: PS417_10465 - tRNA 2-selenouridine synthase, at 2,234,446 to 2,235,540
_10465
PS417_10470: PS417_10470 - membrane protein, at 2,235,651 to 2,236,706
_10470
Position (kb)
2234
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2233.482 kb on + strand at 2233.482 kb on + strand at 2233.482 kb on + strand at 2233.685 kb on + strand, within PS417_10460 at 2233.685 kb on + strand, within PS417_10460 at 2233.685 kb on + strand, within PS417_10460 at 2233.685 kb on + strand, within PS417_10460 at 2233.685 kb on + strand, within PS417_10460 at 2233.685 kb on + strand, within PS417_10460 at 2233.686 kb on - strand, within PS417_10460 at 2233.686 kb on - strand, within PS417_10460 at 2233.686 kb on - strand, within PS417_10460 at 2233.686 kb on - strand, within PS417_10460 at 2233.686 kb on - strand, within PS417_10460 at 2233.892 kb on - strand, within PS417_10460 at 2233.931 kb on + strand, within PS417_10460 at 2233.931 kb on + strand, within PS417_10460 at 2233.931 kb on + strand, within PS417_10460 at 2234.029 kb on + strand, within PS417_10460 at 2234.198 kb on + strand, within PS417_10460 at 2234.199 kb on - strand, within PS417_10460 at 2234.199 kb on - strand, within PS417_10460 at 2234.215 kb on + strand, within PS417_10460 at 2234.216 kb on - strand, within PS417_10460 at 2234.249 kb on + strand, within PS417_10460 at 2234.285 kb on - strand, within PS417_10460 at 2234.434 kb on - strand at 2234.602 kb on + strand, within PS417_10465 at 2234.603 kb on - strand, within PS417_10465 at 2234.817 kb on + strand, within PS417_10465 at 2235.151 kb on + strand, within PS417_10465 at 2235.211 kb on + strand, within PS417_10465 at 2235.211 kb on + strand, within PS417_10465 at 2235.211 kb on + strand, within PS417_10465 at 2235.246 kb on - strand, within PS417_10465 at 2235.304 kb on - strand, within PS417_10465 at 2235.434 kb on - strand at 2235.436 kb on + strand at 2235.437 kb on - strand at 2235.448 kb on + strand at 2235.845 kb on + strand, within PS417_10470 at 2235.849 kb on + strand, within PS417_10470 at 2235.849 kb on + strand, within PS417_10470 at 2236.179 kb on + strand, within PS417_10470 at 2236.371 kb on - strand, within PS417_10470 at 2236.486 kb on + strand, within PS417_10470 at 2236.499 kb on - strand, within PS417_10470
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,233,482 + +0.7 2,233,482 + -0.2 2,233,482 + -0.9 2,233,685 + PS417_10460 0.26 -0.2 2,233,685 + PS417_10460 0.26 -0.2 2,233,685 + PS417_10460 0.26 -1.2 2,233,685 + PS417_10460 0.26 -0.6 2,233,685 + PS417_10460 0.26 -1.7 2,233,685 + PS417_10460 0.26 -0.3 2,233,686 - PS417_10460 0.26 -1.5 2,233,686 - PS417_10460 0.26 +0.6 2,233,686 - PS417_10460 0.26 +0.8 2,233,686 - PS417_10460 0.26 -0.4 2,233,686 - PS417_10460 0.26 -0.5 2,233,892 - PS417_10460 0.46 -0.1 2,233,931 + PS417_10460 0.50 -0.4 2,233,931 + PS417_10460 0.50 +0.2 2,233,931 + PS417_10460 0.50 -0.1 2,234,029 + PS417_10460 0.60 -0.4 2,234,198 + PS417_10460 0.76 -0.4 2,234,199 - PS417_10460 0.76 -0.6 2,234,199 - PS417_10460 0.76 +0.3 2,234,215 + PS417_10460 0.78 +0.6 2,234,216 - PS417_10460 0.78 -0.1 2,234,249 + PS417_10460 0.81 -0.0 2,234,285 - PS417_10460 0.84 -2.7 2,234,434 - -1.0 2,234,602 + PS417_10465 0.14 +0.8 2,234,603 - PS417_10465 0.14 +2.7 2,234,817 + PS417_10465 0.34 -0.0 2,235,151 + PS417_10465 0.64 +0.1 2,235,211 + PS417_10465 0.70 +0.5 2,235,211 + PS417_10465 0.70 -0.3 2,235,211 + PS417_10465 0.70 +0.5 2,235,246 - PS417_10465 0.73 -0.3 2,235,304 - PS417_10465 0.78 +0.5 2,235,434 - -0.4 2,235,436 + +0.3 2,235,437 - -2.0 2,235,448 + +0.3 2,235,845 + PS417_10470 0.18 +1.1 2,235,849 + PS417_10470 0.19 -0.1 2,235,849 + PS417_10470 0.19 +0.1 2,236,179 + PS417_10470 0.50 +0.2 2,236,371 - PS417_10470 0.68 -0.8 2,236,486 + PS417_10470 0.79 +0.2 2,236,499 - PS417_10470 0.80 -0.9
Or see this region's nucleotide sequence