Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10295 and PS417_10300 are separated by 69 nucleotides PS417_10300 and PS417_10305 are separated by 224 nucleotides PS417_10305 and PS417_10310 are separated by 42 nucleotides PS417_10310 and PS417_10315 are separated by 31 nucleotides
PS417_10295: PS417_10295 - chemotaxis protein, at 2,201,450 to 2,203,129
_10295
PS417_10300: PS417_10300 - protease, at 2,203,199 to 2,204,512
_10300
PS417_10305: PS417_10305 - hypothetical protein, at 2,204,737 to 2,205,000
_10305
PS417_10310: PS417_10310 - hypothetical protein, at 2,205,043 to 2,205,255
_10310
PS417_10315: PS417_10315 - cytochrome C biogenesis protein, at 2,205,287 to 2,206,495
_10315
Position (kb)
2203
2204
2205 Strain fitness (log2 ratio)
-2
-1
0
1 at 2202.727 kb on - strand, within PS417_10295 at 2202.742 kb on - strand, within PS417_10295 at 2203.138 kb on - strand at 2203.202 kb on + strand at 2203.202 kb on + strand at 2203.202 kb on + strand at 2203.202 kb on + strand at 2203.257 kb on + strand at 2203.258 kb on - strand at 2203.479 kb on + strand, within PS417_10300 at 2203.480 kb on - strand, within PS417_10300 at 2203.480 kb on - strand, within PS417_10300 at 2203.488 kb on + strand, within PS417_10300 at 2203.528 kb on - strand, within PS417_10300 at 2203.743 kb on + strand at 2203.903 kb on - strand, within PS417_10300 at 2203.957 kb on + strand, within PS417_10300 at 2203.959 kb on + strand at 2203.986 kb on + strand, within PS417_10300 at 2203.987 kb on - strand, within PS417_10300 at 2204.072 kb on + strand, within PS417_10300 at 2204.073 kb on - strand, within PS417_10300 at 2204.624 kb on - strand at 2204.680 kb on + strand at 2204.680 kb on + strand at 2204.680 kb on + strand at 2204.681 kb on - strand at 2204.681 kb on - strand at 2204.756 kb on - strand at 2204.840 kb on - strand, within PS417_10305 at 2204.926 kb on + strand, within PS417_10305 at 2204.963 kb on - strand, within PS417_10305 at 2205.291 kb on + strand at 2205.338 kb on - strand at 2205.471 kb on + strand, within PS417_10315 at 2205.472 kb on - strand, within PS417_10315 at 2205.481 kb on + strand, within PS417_10315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,202,727 - PS417_10295 0.76 +0.5 2,202,742 - PS417_10295 0.77 +0.5 2,203,138 - -1.2 2,203,202 + -0.6 2,203,202 + +0.0 2,203,202 + -1.7 2,203,202 + +1.3 2,203,257 + +0.1 2,203,258 - -1.8 2,203,479 + PS417_10300 0.21 -1.2 2,203,480 - PS417_10300 0.21 -0.1 2,203,480 - PS417_10300 0.21 -0.6 2,203,488 + PS417_10300 0.22 -0.3 2,203,528 - PS417_10300 0.25 -2.1 2,203,743 + +1.0 2,203,903 - PS417_10300 0.54 -1.8 2,203,957 + PS417_10300 0.58 -0.1 2,203,959 + -1.0 2,203,986 + PS417_10300 0.60 +0.2 2,203,987 - PS417_10300 0.60 -1.0 2,204,072 + PS417_10300 0.66 +0.9 2,204,073 - PS417_10300 0.67 -1.2 2,204,624 - +0.6 2,204,680 + +1.1 2,204,680 + +0.6 2,204,680 + -0.4 2,204,681 - +0.6 2,204,681 - -0.7 2,204,756 - +0.7 2,204,840 - PS417_10305 0.39 +0.1 2,204,926 + PS417_10305 0.72 -0.1 2,204,963 - PS417_10305 0.86 -0.6 2,205,291 + +0.4 2,205,338 - +1.5 2,205,471 + PS417_10315 0.15 +0.8 2,205,472 - PS417_10315 0.15 -0.1 2,205,481 + PS417_10315 0.16 +0.4
Or see this region's nucleotide sequence