Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00985 and PS417_00990 are separated by 176 nucleotides PS417_00990 and PS417_00995 are separated by 226 nucleotides PS417_00995 and PS417_01000 are separated by 50 nucleotides
PS417_00985: PS417_00985 - MFS transporter, at 216,493 to 217,662
_00985
PS417_00990: PS417_00990 - LysR family transcriptional regulator, at 217,839 to 218,690
_00990
PS417_00995: PS417_00995 - alpha/beta hydrolase, at 218,917 to 219,498
_00995
PS417_01000: PS417_01000 - Fis family transcriptional regulator, at 219,549 to 220,475
_01000
Position (kb) 217
218
219 Strain fitness (log2 ratio) -2
-1
0
1
2 at 216.980 kb on + strand, within PS417_00985 at 217.010 kb on + strand, within PS417_00985 at 217.034 kb on + strand, within PS417_00985 at 217.257 kb on - strand, within PS417_00985 at 217.478 kb on - strand, within PS417_00985 at 217.650 kb on + strand at 217.683 kb on + strand at 217.684 kb on - strand at 217.765 kb on + strand at 217.766 kb on - strand at 217.981 kb on + strand, within PS417_00990 at 217.982 kb on - strand, within PS417_00990 at 218.040 kb on + strand, within PS417_00990 at 218.040 kb on + strand, within PS417_00990 at 218.516 kb on + strand, within PS417_00990 at 218.516 kb on + strand, within PS417_00990 at 218.517 kb on - strand, within PS417_00990 at 218.517 kb on - strand, within PS417_00990 at 218.540 kb on - strand, within PS417_00990 at 218.610 kb on + strand at 218.610 kb on + strand at 218.610 kb on + strand at 218.627 kb on - strand at 218.627 kb on - strand at 218.839 kb on - strand at 218.874 kb on + strand at 218.875 kb on + strand at 218.875 kb on + strand at 218.875 kb on + strand at 218.877 kb on + strand at 218.878 kb on - strand at 219.188 kb on + strand, within PS417_00995 at 219.399 kb on + strand, within PS417_00995 at 219.446 kb on - strand at 219.452 kb on - strand at 219.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 216,980 + PS417_00985 0.42 +0.5 217,010 + PS417_00985 0.44 -1.0 217,034 + PS417_00985 0.46 +0.0 217,257 - PS417_00985 0.65 +1.0 217,478 - PS417_00985 0.84 -0.3 217,650 + -0.2 217,683 + +0.8 217,684 - +1.2 217,765 + -0.2 217,766 - -0.2 217,981 + PS417_00990 0.17 +0.8 217,982 - PS417_00990 0.17 -0.1 218,040 + PS417_00990 0.24 +0.1 218,040 + PS417_00990 0.24 +0.4 218,516 + PS417_00990 0.79 -0.8 218,516 + PS417_00990 0.79 +1.9 218,517 - PS417_00990 0.80 +0.2 218,517 - PS417_00990 0.80 +0.9 218,540 - PS417_00990 0.82 +1.5 218,610 + +0.6 218,610 + -0.0 218,610 + +0.5 218,627 - -2.2 218,627 - -0.3 218,839 - +0.5 218,874 + -0.6 218,875 + -0.1 218,875 + +0.4 218,875 + +0.4 218,877 + +0.5 218,878 - +0.7 219,188 + PS417_00995 0.47 -0.4 219,399 + PS417_00995 0.83 +0.0 219,446 - -1.6 219,452 - +0.7 219,522 + -2.1
Or see this region's nucleotide sequence