Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07840 and PS417_07845 are separated by 179 nucleotides PS417_07845 and PS417_07850 are separated by 2 nucleotides PS417_07850 and PS417_07855 are separated by 70 nucleotides PS417_07855 and PS417_07860 are separated by 99 nucleotides
PS417_07840: PS417_07840 - hypothetical protein, at 1,721,758 to 1,721,973
_07840
PS417_07845: PS417_07845 - transcriptional regulator, at 1,722,153 to 1,722,518
_07845
PS417_07850: PS417_07850 - hypothetical protein, at 1,722,521 to 1,723,219
_07850
PS417_07855: PS417_07855 - hypothetical protein, at 1,723,290 to 1,723,565
_07855
PS417_07860: PS417_07860 - hypothetical protein, at 1,723,665 to 1,724,480
_07860
Position (kb)
1722
1723
1724 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1721.528 kb on + strand at 1721.528 kb on + strand at 1721.529 kb on - strand at 1721.529 kb on - strand at 1721.623 kb on + strand at 1721.623 kb on + strand at 1721.624 kb on - strand at 1721.639 kb on - strand at 1721.641 kb on - strand at 1721.664 kb on + strand at 1721.664 kb on + strand at 1721.665 kb on - strand at 1721.665 kb on - strand at 1721.665 kb on - strand at 1721.684 kb on - strand at 1721.725 kb on + strand at 1721.759 kb on + strand at 1721.759 kb on + strand at 1721.872 kb on + strand, within PS417_07840 at 1721.873 kb on - strand, within PS417_07840 at 1721.879 kb on + strand, within PS417_07840 at 1721.879 kb on + strand, within PS417_07840 at 1721.879 kb on + strand, within PS417_07840 at 1721.880 kb on - strand, within PS417_07840 at 1721.880 kb on - strand, within PS417_07840 at 1721.955 kb on + strand at 1722.030 kb on - strand at 1722.056 kb on + strand at 1722.056 kb on + strand at 1722.069 kb on + strand at 1722.070 kb on - strand at 1722.089 kb on + strand at 1722.089 kb on + strand at 1722.089 kb on + strand at 1722.115 kb on + strand at 1722.125 kb on - strand at 1722.131 kb on + strand at 1722.171 kb on + strand at 1722.171 kb on + strand at 1722.172 kb on - strand at 1722.173 kb on + strand at 1722.174 kb on - strand at 1722.174 kb on - strand at 1722.368 kb on + strand, within PS417_07845 at 1722.402 kb on + strand, within PS417_07845 at 1722.517 kb on - strand at 1722.559 kb on - strand at 1722.559 kb on - strand at 1722.575 kb on + strand at 1722.575 kb on + strand at 1722.576 kb on - strand at 1722.583 kb on - strand at 1722.734 kb on + strand, within PS417_07850 at 1722.735 kb on - strand, within PS417_07850 at 1722.872 kb on + strand, within PS417_07850 at 1722.873 kb on - strand at 1722.873 kb on - strand, within PS417_07850 at 1722.873 kb on - strand, within PS417_07850 at 1722.878 kb on + strand, within PS417_07850 at 1723.089 kb on + strand, within PS417_07850 at 1723.168 kb on + strand at 1723.214 kb on + strand at 1723.214 kb on + strand at 1723.217 kb on + strand at 1723.218 kb on - strand at 1723.249 kb on + strand at 1723.258 kb on + strand at 1723.258 kb on + strand at 1723.259 kb on - strand at 1723.270 kb on + strand at 1723.276 kb on + strand at 1723.335 kb on + strand, within PS417_07855 at 1723.350 kb on + strand, within PS417_07855 at 1723.351 kb on - strand, within PS417_07855 at 1723.393 kb on + strand, within PS417_07855 at 1723.670 kb on + strand at 1723.671 kb on - strand at 1723.825 kb on - strand, within PS417_07860 at 1723.834 kb on - strand at 1723.834 kb on - strand, within PS417_07860 at 1723.857 kb on + strand, within PS417_07860 at 1723.860 kb on - strand, within PS417_07860 at 1723.991 kb on + strand, within PS417_07860 at 1724.109 kb on + strand, within PS417_07860 at 1724.110 kb on - strand, within PS417_07860 at 1724.118 kb on - strand, within PS417_07860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,721,528 + +0.1 1,721,528 + -1.9 1,721,529 - +0.7 1,721,529 - -0.5 1,721,623 + -0.3 1,721,623 + +1.0 1,721,624 - -0.6 1,721,639 - +0.1 1,721,641 - +0.1 1,721,664 + +1.4 1,721,664 + +0.5 1,721,665 - -0.5 1,721,665 - -0.0 1,721,665 - +0.4 1,721,684 - +0.2 1,721,725 + +0.4 1,721,759 + -0.2 1,721,759 + +0.2 1,721,872 + PS417_07840 0.53 +0.7 1,721,873 - PS417_07840 0.53 +1.7 1,721,879 + PS417_07840 0.56 -0.4 1,721,879 + PS417_07840 0.56 +1.0 1,721,879 + PS417_07840 0.56 +1.9 1,721,880 - PS417_07840 0.56 -1.2 1,721,880 - PS417_07840 0.56 +4.3 1,721,955 + -0.5 1,722,030 - +0.4 1,722,056 + +0.5 1,722,056 + +2.9 1,722,069 + -1.2 1,722,070 - -1.4 1,722,089 + +0.6 1,722,089 + +3.3 1,722,089 + -0.1 1,722,115 + +3.7 1,722,125 - +0.8 1,722,131 + +0.8 1,722,171 + +0.6 1,722,171 + -0.1 1,722,172 - -0.2 1,722,173 + +0.7 1,722,174 - +0.6 1,722,174 - -2.0 1,722,368 + PS417_07845 0.59 +1.0 1,722,402 + PS417_07845 0.68 -1.1 1,722,517 - -0.3 1,722,559 - +0.6 1,722,559 - -0.3 1,722,575 + -0.0 1,722,575 + +1.1 1,722,576 - -2.9 1,722,583 - +1.3 1,722,734 + PS417_07850 0.30 -0.0 1,722,735 - PS417_07850 0.31 +0.8 1,722,872 + PS417_07850 0.50 +0.8 1,722,873 - +0.1 1,722,873 - PS417_07850 0.50 +0.9 1,722,873 - PS417_07850 0.50 +1.0 1,722,878 + PS417_07850 0.51 +0.6 1,723,089 + PS417_07850 0.81 +0.7 1,723,168 + -3.2 1,723,214 + +0.6 1,723,214 + +0.3 1,723,217 + +0.3 1,723,218 - +2.1 1,723,249 + +0.4 1,723,258 + +1.5 1,723,258 + -0.7 1,723,259 - +1.4 1,723,270 + -0.0 1,723,276 + -0.1 1,723,335 + PS417_07855 0.16 +0.0 1,723,350 + PS417_07855 0.22 +0.8 1,723,351 - PS417_07855 0.22 -0.7 1,723,393 + PS417_07855 0.37 +0.7 1,723,670 + +0.1 1,723,671 - +0.6 1,723,825 - PS417_07860 0.20 +0.4 1,723,834 - -1.1 1,723,834 - PS417_07860 0.21 -1.7 1,723,857 + PS417_07860 0.24 -0.4 1,723,860 - PS417_07860 0.24 +0.5 1,723,991 + PS417_07860 0.40 -2.8 1,724,109 + PS417_07860 0.54 -0.4 1,724,110 - PS417_07860 0.55 +1.4 1,724,118 - PS417_07860 0.56 -2.5
Or see this region's nucleotide sequence