Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06875 and PS417_06880 overlap by 4 nucleotides PS417_06880 and PS417_06885 are separated by 200 nucleotides PS417_06885 and PS417_06890 are separated by 28 nucleotides PS417_06890 and PS417_06895 are separated by 105 nucleotides
PS417_06875: PS417_06875 - iron dicitrate transport regulator FecR, at 1,522,730 to 1,523,701
_06875
PS417_06880: PS417_06880 - RNA polymerase sigma factor, at 1,523,698 to 1,524,237
_06880
PS417_06885: PS417_06885 - dihydrodipicolinate synthase, at 1,524,438 to 1,525,310
_06885
PS417_06890: PS417_06890 - aldolase, at 1,525,339 to 1,526,121
_06890
PS417_06895: PS417_06895 - DNA-binding protein, at 1,526,227 to 1,526,754
_06895
Position (kb)
1524
1525
1526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1524.171 kb on - strand, within PS417_06880 at 1524.171 kb on - strand, within PS417_06880 at 1524.177 kb on + strand, within PS417_06880 at 1524.177 kb on + strand, within PS417_06880 at 1524.177 kb on + strand, within PS417_06880 at 1524.177 kb on + strand, within PS417_06880 at 1524.184 kb on - strand at 1524.185 kb on + strand at 1524.185 kb on + strand at 1524.185 kb on + strand at 1524.185 kb on + strand at 1524.185 kb on + strand at 1524.185 kb on + strand at 1524.186 kb on - strand at 1524.646 kb on + strand at 1524.646 kb on + strand, within PS417_06885 at 1524.646 kb on + strand, within PS417_06885 at 1524.646 kb on + strand, within PS417_06885 at 1524.647 kb on - strand, within PS417_06885 at 1524.647 kb on - strand, within PS417_06885 at 1524.648 kb on + strand, within PS417_06885 at 1524.749 kb on - strand, within PS417_06885 at 1524.749 kb on - strand, within PS417_06885 at 1524.899 kb on - strand, within PS417_06885 at 1525.148 kb on - strand, within PS417_06885 at 1525.276 kb on + strand at 1525.277 kb on - strand at 1525.383 kb on - strand at 1525.385 kb on + strand at 1525.385 kb on + strand at 1525.386 kb on - strand at 1525.386 kb on - strand at 1525.386 kb on - strand at 1525.386 kb on - strand at 1525.682 kb on + strand, within PS417_06890 at 1525.683 kb on - strand, within PS417_06890 at 1525.701 kb on + strand, within PS417_06890 at 1525.925 kb on + strand, within PS417_06890 at 1525.926 kb on - strand, within PS417_06890 at 1525.928 kb on + strand, within PS417_06890 at 1525.929 kb on - strand, within PS417_06890 at 1525.929 kb on - strand, within PS417_06890 at 1526.040 kb on + strand, within PS417_06890 at 1526.040 kb on + strand, within PS417_06890 at 1526.041 kb on - strand, within PS417_06890 at 1526.041 kb on - strand at 1526.108 kb on - strand at 1526.268 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,524,171 - PS417_06880 0.88 +1.9 1,524,171 - PS417_06880 0.88 +1.1 1,524,177 + PS417_06880 0.89 -0.1 1,524,177 + PS417_06880 0.89 -0.1 1,524,177 + PS417_06880 0.89 -1.2 1,524,177 + PS417_06880 0.89 -1.1 1,524,184 - -1.2 1,524,185 + +0.7 1,524,185 + -1.1 1,524,185 + -0.2 1,524,185 + -0.3 1,524,185 + -0.3 1,524,185 + +0.1 1,524,186 - -1.4 1,524,646 + -0.3 1,524,646 + PS417_06885 0.24 -1.9 1,524,646 + PS417_06885 0.24 -0.4 1,524,646 + PS417_06885 0.24 -0.6 1,524,647 - PS417_06885 0.24 -1.0 1,524,647 - PS417_06885 0.24 -0.9 1,524,648 + PS417_06885 0.24 -1.6 1,524,749 - PS417_06885 0.36 +0.6 1,524,749 - PS417_06885 0.36 -2.1 1,524,899 - PS417_06885 0.53 -0.6 1,525,148 - PS417_06885 0.81 +0.1 1,525,276 + -1.6 1,525,277 - -1.4 1,525,383 - -0.7 1,525,385 + +0.4 1,525,385 + -0.3 1,525,386 - +0.7 1,525,386 - +3.1 1,525,386 - +0.3 1,525,386 - +3.4 1,525,682 + PS417_06890 0.44 -0.1 1,525,683 - PS417_06890 0.44 +0.6 1,525,701 + PS417_06890 0.46 -3.0 1,525,925 + PS417_06890 0.75 +2.5 1,525,926 - PS417_06890 0.75 -0.2 1,525,928 + PS417_06890 0.75 +0.1 1,525,929 - PS417_06890 0.75 +0.6 1,525,929 - PS417_06890 0.75 +0.2 1,526,040 + PS417_06890 0.90 -2.7 1,526,040 + PS417_06890 0.90 -2.0 1,526,041 - PS417_06890 0.90 -1.7 1,526,041 - +1.7 1,526,108 - +0.2 1,526,268 + -1.0
Or see this region's nucleotide sequence