Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06330 and PS417_06335 are separated by 8 nucleotides PS417_06335 and PS417_06340 are separated by 54 nucleotides PS417_06340 and PS417_06345 overlap by 4 nucleotides
PS417_06330: PS417_06330 - S-(hydroxymethyl)glutathione dehydrogenase, at 1,406,099 to 1,407,211
_06330
PS417_06335: PS417_06335 - S-formylglutathione hydrolase, at 1,407,220 to 1,408,065
_06335
PS417_06340: PS417_06340 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,408,120 to 1,408,593
_06340
PS417_06345: PS417_06345 - pseudouridine synthase, at 1,408,590 to 1,409,648
_06345
Position (kb) 1407
1408
1409 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1406.234 kb on + strand, within PS417_06330 at 1406.236 kb on + strand, within PS417_06330 at 1406.363 kb on + strand at 1406.480 kb on + strand, within PS417_06330 at 1406.504 kb on + strand, within PS417_06330 at 1406.570 kb on - strand, within PS417_06330 at 1407.274 kb on + strand at 1407.541 kb on + strand, within PS417_06335 at 1407.592 kb on + strand, within PS417_06335 at 1407.592 kb on + strand at 1407.593 kb on - strand, within PS417_06335 at 1407.593 kb on - strand, within PS417_06335 at 1407.593 kb on - strand, within PS417_06335 at 1407.989 kb on - strand at 1408.000 kb on + strand at 1408.001 kb on - strand at 1408.001 kb on - strand at 1408.815 kb on + strand, within PS417_06345 at 1408.815 kb on + strand, within PS417_06345 at 1408.816 kb on - strand, within PS417_06345 at 1408.816 kb on - strand, within PS417_06345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,406,234 + PS417_06330 0.12 +1.7 1,406,236 + PS417_06330 0.12 -0.5 1,406,363 + +0.7 1,406,480 + PS417_06330 0.34 -0.0 1,406,504 + PS417_06330 0.36 -1.3 1,406,570 - PS417_06330 0.42 -1.9 1,407,274 + -1.2 1,407,541 + PS417_06335 0.38 -0.9 1,407,592 + PS417_06335 0.44 +1.4 1,407,592 + -0.3 1,407,593 - PS417_06335 0.44 -2.7 1,407,593 - PS417_06335 0.44 -1.7 1,407,593 - PS417_06335 0.44 -3.3 1,407,989 - -0.6 1,408,000 + +0.4 1,408,001 - -0.1 1,408,001 - -0.1 1,408,815 + PS417_06345 0.21 +0.9 1,408,815 + PS417_06345 0.21 +0.4 1,408,816 - PS417_06345 0.21 +0.7 1,408,816 - PS417_06345 0.21 -1.3
Or see this region's nucleotide sequence