Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06005 and PS417_06010 are separated by 106 nucleotides PS417_06010 and PS417_06015 are separated by 146 nucleotides PS417_06015 and PS417_06020 are separated by 21 nucleotides
PS417_06005: PS417_06005 - lysyl-tRNA synthetase, at 1,334,932 to 1,336,431
_06005
PS417_06010: PS417_06010 - TetR family transcriptional regulator, at 1,336,538 to 1,337,254
_06010
PS417_06015: PS417_06015 - hypothetical protein, at 1,337,401 to 1,337,949
_06015
PS417_06020: PS417_06020 - membrane protein, at 1,337,971 to 1,339,248
_06020
Position (kb)
1337
1338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1336.459 kb on - strand at 1336.459 kb on - strand at 1336.459 kb on - strand at 1336.520 kb on + strand at 1336.520 kb on + strand at 1336.521 kb on - strand at 1336.760 kb on + strand, within PS417_06010 at 1336.760 kb on + strand, within PS417_06010 at 1336.880 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.947 kb on - strand, within PS417_06010 at 1337.160 kb on - strand, within PS417_06010 at 1337.225 kb on + strand at 1337.274 kb on + strand at 1337.274 kb on + strand at 1337.424 kb on - strand at 1337.587 kb on + strand, within PS417_06015 at 1337.587 kb on + strand, within PS417_06015 at 1337.588 kb on - strand, within PS417_06015 at 1337.589 kb on + strand, within PS417_06015 at 1337.590 kb on - strand, within PS417_06015 at 1337.607 kb on + strand, within PS417_06015 at 1337.608 kb on - strand, within PS417_06015 at 1337.635 kb on + strand, within PS417_06015 at 1337.635 kb on + strand, within PS417_06015 at 1337.635 kb on + strand, within PS417_06015 at 1337.635 kb on + strand, within PS417_06015 at 1337.636 kb on - strand, within PS417_06015 at 1337.643 kb on + strand at 1337.644 kb on - strand, within PS417_06015 at 1337.671 kb on + strand, within PS417_06015 at 1337.758 kb on + strand, within PS417_06015 at 1337.759 kb on - strand, within PS417_06015 at 1337.910 kb on - strand at 1337.947 kb on + strand at 1337.947 kb on + strand at 1337.948 kb on - strand at 1337.955 kb on - strand at 1338.127 kb on + strand, within PS417_06020 at 1338.128 kb on - strand, within PS417_06020 at 1338.131 kb on - strand, within PS417_06020 at 1338.331 kb on + strand at 1338.403 kb on + strand, within PS417_06020 at 1338.404 kb on - strand, within PS417_06020 at 1338.404 kb on - strand, within PS417_06020 at 1338.525 kb on + strand, within PS417_06020 at 1338.526 kb on - strand, within PS417_06020 at 1338.526 kb on - strand, within PS417_06020 at 1338.581 kb on - strand, within PS417_06020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,336,459 - +0.1 1,336,459 - +0.1 1,336,459 - -1.7 1,336,520 + +0.1 1,336,520 + +1.2 1,336,521 - -0.5 1,336,760 + PS417_06010 0.31 +0.3 1,336,760 + PS417_06010 0.31 -0.1 1,336,880 + PS417_06010 0.48 +0.2 1,336,946 + PS417_06010 0.57 +1.0 1,336,946 + PS417_06010 0.57 -1.5 1,336,946 + PS417_06010 0.57 -2.1 1,336,947 - PS417_06010 0.57 -0.1 1,337,160 - PS417_06010 0.87 -0.6 1,337,225 + +0.4 1,337,274 + -0.6 1,337,274 + -2.0 1,337,424 - -3.4 1,337,587 + PS417_06015 0.34 -2.7 1,337,587 + PS417_06015 0.34 -1.1 1,337,588 - PS417_06015 0.34 +2.3 1,337,589 + PS417_06015 0.34 +0.2 1,337,590 - PS417_06015 0.34 -0.3 1,337,607 + PS417_06015 0.38 -0.8 1,337,608 - PS417_06015 0.38 +0.4 1,337,635 + PS417_06015 0.43 -2.6 1,337,635 + PS417_06015 0.43 -1.3 1,337,635 + PS417_06015 0.43 -3.0 1,337,635 + PS417_06015 0.43 -0.2 1,337,636 - PS417_06015 0.43 -0.6 1,337,643 + -0.3 1,337,644 - PS417_06015 0.44 -0.5 1,337,671 + PS417_06015 0.49 -0.2 1,337,758 + PS417_06015 0.65 -0.5 1,337,759 - PS417_06015 0.65 +0.0 1,337,910 - +0.8 1,337,947 + +0.2 1,337,947 + +0.2 1,337,948 - +0.4 1,337,955 - -0.8 1,338,127 + PS417_06020 0.12 -0.5 1,338,128 - PS417_06020 0.12 -2.0 1,338,131 - PS417_06020 0.13 +0.5 1,338,331 + -0.6 1,338,403 + PS417_06020 0.34 +0.0 1,338,404 - PS417_06020 0.34 +0.3 1,338,404 - PS417_06020 0.34 +0.2 1,338,525 + PS417_06020 0.43 -0.6 1,338,526 - PS417_06020 0.43 -1.0 1,338,526 - PS417_06020 0.43 -0.7 1,338,581 - PS417_06020 0.48 +0.3
Or see this region's nucleotide sequence