Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05460 and PS417_05465 are separated by 206 nucleotides PS417_05465 and PS417_05470 are separated by 55 nucleotides PS417_05470 and PS417_05475 are separated by 82 nucleotides
PS417_05460: PS417_05460 - hypothetical protein, at 1,225,879 to 1,231,530
_05460
PS417_05465: PS417_05465 - alpha/beta hydrolase, at 1,231,737 to 1,232,756
_05465
PS417_05470: PS417_05470 - DSBA oxidoreductase, at 1,232,812 to 1,233,465
_05470
PS417_05475: PS417_05475 - arginase, at 1,233,548 to 1,234,468
_05475
Position (kb)
1231
1232
1233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1230.782 kb on + strand at 1230.783 kb on - strand, within PS417_05460 at 1230.884 kb on + strand, within PS417_05460 at 1230.884 kb on + strand, within PS417_05460 at 1230.885 kb on - strand, within PS417_05460 at 1231.016 kb on + strand at 1231.135 kb on + strand at 1231.614 kb on + strand at 1231.614 kb on + strand at 1231.614 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.618 kb on + strand at 1231.619 kb on - strand at 1231.619 kb on - strand at 1231.619 kb on - strand at 1231.619 kb on - strand at 1231.619 kb on - strand at 1231.619 kb on - strand at 1231.708 kb on + strand at 1231.708 kb on + strand at 1231.749 kb on + strand at 1231.749 kb on + strand at 1231.753 kb on + strand at 1231.918 kb on + strand, within PS417_05465 at 1231.918 kb on + strand, within PS417_05465 at 1231.919 kb on - strand, within PS417_05465 at 1231.996 kb on + strand, within PS417_05465 at 1231.996 kb on + strand, within PS417_05465 at 1231.997 kb on - strand, within PS417_05465 at 1231.997 kb on - strand, within PS417_05465 at 1231.997 kb on - strand, within PS417_05465 at 1232.058 kb on + strand, within PS417_05465 at 1232.088 kb on + strand, within PS417_05465 at 1232.159 kb on - strand, within PS417_05465 at 1232.591 kb on + strand, within PS417_05465 at 1232.682 kb on + strand at 1232.765 kb on + strand at 1232.765 kb on + strand at 1232.766 kb on - strand at 1232.766 kb on - strand at 1233.109 kb on + strand, within PS417_05470 at 1233.110 kb on - strand, within PS417_05470 at 1233.110 kb on - strand, within PS417_05470 at 1233.166 kb on + strand, within PS417_05470 at 1233.166 kb on + strand, within PS417_05470 at 1233.167 kb on - strand, within PS417_05470 at 1233.167 kb on - strand, within PS417_05470 at 1233.305 kb on - strand, within PS417_05470 at 1233.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,230,782 + +0.1 1,230,783 - PS417_05460 0.87 -1.3 1,230,884 + PS417_05460 0.89 +0.3 1,230,884 + PS417_05460 0.89 +0.5 1,230,885 - PS417_05460 0.89 -0.5 1,231,016 + +3.0 1,231,135 + +0.5 1,231,614 + +1.9 1,231,614 + +0.4 1,231,614 + +0.2 1,231,618 + -0.4 1,231,618 + +2.8 1,231,618 + -1.0 1,231,618 + -1.5 1,231,618 + -1.1 1,231,618 + -2.0 1,231,618 + -0.9 1,231,619 - -1.5 1,231,619 - +0.6 1,231,619 - +0.3 1,231,619 - -1.1 1,231,619 - +2.9 1,231,619 - -1.9 1,231,708 + -0.1 1,231,708 + -0.0 1,231,749 + -0.6 1,231,749 + +0.7 1,231,753 + -1.4 1,231,918 + PS417_05465 0.18 -1.7 1,231,918 + PS417_05465 0.18 -0.3 1,231,919 - PS417_05465 0.18 +2.4 1,231,996 + PS417_05465 0.25 -1.3 1,231,996 + PS417_05465 0.25 +1.1 1,231,997 - PS417_05465 0.25 -0.2 1,231,997 - PS417_05465 0.25 +0.2 1,231,997 - PS417_05465 0.25 +0.3 1,232,058 + PS417_05465 0.31 -1.7 1,232,088 + PS417_05465 0.34 +0.1 1,232,159 - PS417_05465 0.41 +1.8 1,232,591 + PS417_05465 0.84 +1.3 1,232,682 + +0.4 1,232,765 + -2.0 1,232,765 + +1.7 1,232,766 - -0.6 1,232,766 - -1.2 1,233,109 + PS417_05470 0.45 +0.4 1,233,110 - PS417_05470 0.46 -0.9 1,233,110 - PS417_05470 0.46 +2.1 1,233,166 + PS417_05470 0.54 -0.7 1,233,166 + PS417_05470 0.54 +0.2 1,233,167 - PS417_05470 0.54 -0.9 1,233,167 - PS417_05470 0.54 -3.2 1,233,305 - PS417_05470 0.75 -0.7 1,233,553 + +2.8
Or see this region's nucleotide sequence