Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04705 and PS417_04710 are separated by 163 nucleotides PS417_04710 and PS417_04715 are separated by 43 nucleotides PS417_04715 and PS417_04720 are separated by 107 nucleotides PS417_04720 and PS417_04725 overlap by 4 nucleotides
PS417_04705: PS417_04705 - hypothetical protein, at 1,045,393 to 1,045,626
_04705
PS417_04710: PS417_04710 - amino acid ABC transporter substrate-binding protein, at 1,045,790 to 1,046,560
_04710
PS417_04715: PS417_04715 - Fe-S oxidoreductase, at 1,046,604 to 1,047,347
_04715
PS417_04720: PS417_04720 - GNAT family acetyltransferase, at 1,047,455 to 1,047,937
_04720
PS417_04725: PS417_04725 - chemotaxis protein CheW, at 1,047,934 to 1,048,836
_04725
Position (kb)
1046
1047
1048 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1045.640 kb on + strand at 1045.688 kb on + strand at 1045.689 kb on - strand at 1045.800 kb on + strand at 1045.800 kb on + strand at 1045.801 kb on - strand at 1045.801 kb on - strand at 1045.813 kb on - strand at 1045.813 kb on - strand at 1045.949 kb on - strand, within PS417_04710 at 1045.993 kb on + strand, within PS417_04710 at 1045.994 kb on - strand, within PS417_04710 at 1045.994 kb on - strand, within PS417_04710 at 1046.065 kb on + strand, within PS417_04710 at 1046.206 kb on - strand, within PS417_04710 at 1046.319 kb on + strand, within PS417_04710 at 1046.387 kb on + strand, within PS417_04710 at 1046.387 kb on + strand, within PS417_04710 at 1046.388 kb on - strand, within PS417_04710 at 1046.413 kb on + strand, within PS417_04710 at 1046.453 kb on - strand, within PS417_04710 at 1046.503 kb on + strand at 1046.503 kb on + strand at 1046.566 kb on + strand at 1046.566 kb on + strand at 1046.633 kb on - strand at 1046.633 kb on - strand at 1046.742 kb on + strand, within PS417_04715 at 1046.743 kb on - strand, within PS417_04715 at 1046.835 kb on + strand, within PS417_04715 at 1046.922 kb on + strand, within PS417_04715 at 1046.922 kb on + strand, within PS417_04715 at 1046.923 kb on - strand, within PS417_04715 at 1046.923 kb on - strand, within PS417_04715 at 1046.923 kb on - strand, within PS417_04715 at 1047.026 kb on - strand, within PS417_04715 at 1047.055 kb on + strand at 1047.367 kb on + strand at 1047.367 kb on + strand at 1047.367 kb on + strand at 1047.368 kb on - strand at 1047.368 kb on - strand at 1047.440 kb on - strand at 1047.467 kb on + strand at 1047.468 kb on - strand at 1047.468 kb on - strand at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.507 kb on + strand, within PS417_04720 at 1047.508 kb on - strand, within PS417_04720 at 1047.982 kb on - strand at 1048.085 kb on + strand, within PS417_04725 at 1048.085 kb on + strand, within PS417_04725 at 1048.085 kb on + strand, within PS417_04725 at 1048.086 kb on - strand, within PS417_04725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,045,640 + -0.7 1,045,688 + +0.9 1,045,689 - -0.7 1,045,800 + +0.5 1,045,800 + +0.9 1,045,801 - -0.0 1,045,801 - +0.9 1,045,813 - +1.3 1,045,813 - +0.8 1,045,949 - PS417_04710 0.21 +0.2 1,045,993 + PS417_04710 0.26 -1.1 1,045,994 - PS417_04710 0.26 +1.1 1,045,994 - PS417_04710 0.26 -0.0 1,046,065 + PS417_04710 0.36 +0.1 1,046,206 - PS417_04710 0.54 -1.3 1,046,319 + PS417_04710 0.69 +0.2 1,046,387 + PS417_04710 0.77 -0.7 1,046,387 + PS417_04710 0.77 +0.1 1,046,388 - PS417_04710 0.78 -0.6 1,046,413 + PS417_04710 0.81 -0.6 1,046,453 - PS417_04710 0.86 -1.4 1,046,503 + +0.5 1,046,503 + +1.1 1,046,566 + +0.6 1,046,566 + +0.9 1,046,633 - -0.4 1,046,633 - -0.5 1,046,742 + PS417_04715 0.19 +0.4 1,046,743 - PS417_04715 0.19 +2.0 1,046,835 + PS417_04715 0.31 -0.4 1,046,922 + PS417_04715 0.43 +1.6 1,046,922 + PS417_04715 0.43 -0.4 1,046,923 - PS417_04715 0.43 +2.1 1,046,923 - PS417_04715 0.43 +0.0 1,046,923 - PS417_04715 0.43 -1.5 1,047,026 - PS417_04715 0.57 -0.2 1,047,055 + -1.8 1,047,367 + -2.5 1,047,367 + -1.8 1,047,367 + +0.1 1,047,368 - -0.9 1,047,368 - -1.5 1,047,440 - -1.6 1,047,467 + +0.2 1,047,468 - -0.3 1,047,468 - +0.2 1,047,470 + -0.3 1,047,470 + -0.4 1,047,470 + +0.2 1,047,471 - +0.8 1,047,471 - -0.3 1,047,471 - -0.3 1,047,471 - -0.2 1,047,507 + PS417_04720 0.11 -1.4 1,047,508 - PS417_04720 0.11 -3.1 1,047,982 - -0.0 1,048,085 + PS417_04725 0.17 -2.1 1,048,085 + PS417_04725 0.17 +0.4 1,048,085 + PS417_04725 0.17 -0.3 1,048,086 - PS417_04725 0.17 +0.3
Or see this region's nucleotide sequence