Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03795 and PS417_03800 are separated by 11 nucleotides PS417_03800 and PS417_03805 are separated by 62 nucleotides PS417_03805 and PS417_03810 overlap by 14 nucleotides PS417_03810 and PS417_03815 are separated by 124 nucleotides
PS417_03795: PS417_03795 - pilus assembly protein PilX, at 857,947 to 858,477
_03795
PS417_03800: PS417_03800 - pilus assembly protein, at 858,489 to 858,890
_03800
PS417_03805: PS417_03805 - D-amino acid oxidase, at 858,953 to 860,062
_03805
PS417_03810: PS417_03810 - hypothetical protein, at 860,049 to 860,282
_03810
PS417_03815: PS417_03815 - DNA transporter, at 860,407 to 861,432
_03815
Position (kb) 858
859
860
861 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 857.965 kb on - strand at 857.965 kb on - strand at 858.097 kb on - strand, within PS417_03795 at 858.259 kb on - strand, within PS417_03795 at 858.330 kb on - strand, within PS417_03795 at 858.412 kb on + strand at 858.413 kb on - strand at 858.423 kb on - strand, within PS417_03795 at 858.423 kb on - strand, within PS417_03795 at 858.423 kb on - strand, within PS417_03795 at 858.512 kb on + strand at 858.513 kb on - strand at 858.661 kb on - strand, within PS417_03800 at 858.681 kb on - strand at 858.681 kb on - strand, within PS417_03800 at 858.720 kb on - strand, within PS417_03800 at 858.720 kb on - strand, within PS417_03800 at 858.765 kb on - strand, within PS417_03800 at 858.972 kb on + strand at 859.113 kb on - strand, within PS417_03805 at 859.113 kb on - strand, within PS417_03805 at 859.124 kb on + strand, within PS417_03805 at 859.359 kb on - strand, within PS417_03805 at 859.373 kb on + strand, within PS417_03805 at 859.374 kb on - strand, within PS417_03805 at 859.506 kb on + strand, within PS417_03805 at 859.506 kb on + strand, within PS417_03805 at 859.507 kb on - strand, within PS417_03805 at 859.507 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.889 kb on + strand, within PS417_03805 at 859.889 kb on + strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 860.061 kb on - strand at 860.099 kb on - strand, within PS417_03810 at 860.099 kb on - strand, within PS417_03810 at 860.239 kb on - strand at 860.348 kb on + strand at 860.349 kb on - strand at 860.349 kb on - strand at 860.349 kb on - strand at 860.433 kb on - strand at 860.433 kb on - strand at 860.552 kb on + strand, within PS417_03815 at 860.630 kb on + strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 857,965 - +1.3 857,965 - -1.4 858,097 - PS417_03795 0.28 +0.7 858,259 - PS417_03795 0.59 +0.2 858,330 - PS417_03795 0.72 -0.6 858,412 + +0.6 858,413 - +0.0 858,423 - PS417_03795 0.90 -1.1 858,423 - PS417_03795 0.90 -1.4 858,423 - PS417_03795 0.90 +0.9 858,512 + +0.2 858,513 - +0.7 858,661 - PS417_03800 0.43 +0.7 858,681 - -2.0 858,681 - PS417_03800 0.48 +0.5 858,720 - PS417_03800 0.57 +1.2 858,720 - PS417_03800 0.57 -0.0 858,765 - PS417_03800 0.69 -0.4 858,972 + +0.2 859,113 - PS417_03805 0.14 +0.5 859,113 - PS417_03805 0.14 +0.5 859,124 + PS417_03805 0.15 +5.4 859,359 - PS417_03805 0.37 +0.0 859,373 + PS417_03805 0.38 +0.1 859,374 - PS417_03805 0.38 -0.1 859,506 + PS417_03805 0.50 -0.8 859,506 + PS417_03805 0.50 -2.9 859,507 - PS417_03805 0.50 -0.5 859,507 - PS417_03805 0.50 +1.1 859,592 - PS417_03805 0.58 +0.7 859,592 - PS417_03805 0.58 +0.7 859,592 - PS417_03805 0.58 -0.9 859,889 + PS417_03805 0.84 -0.2 859,889 + PS417_03805 0.84 +0.1 859,890 - PS417_03805 0.84 +0.6 859,890 - PS417_03805 0.84 +0.5 859,890 - PS417_03805 0.84 +1.2 859,890 - PS417_03805 0.84 -0.3 859,890 - PS417_03805 0.84 -0.1 860,061 - -0.1 860,099 - PS417_03810 0.21 -1.4 860,099 - PS417_03810 0.21 -0.3 860,239 - -0.4 860,348 + +0.6 860,349 - +0.3 860,349 - +0.7 860,349 - -0.7 860,433 - +0.2 860,433 - -2.7 860,552 + PS417_03815 0.14 -0.6 860,630 + PS417_03815 0.22 +1.8 860,631 - PS417_03815 0.22 -0.9 860,631 - PS417_03815 0.22 +1.4 860,631 - PS417_03815 0.22 -0.5 860,631 - PS417_03815 0.22 -2.2
Or see this region's nucleotide sequence