Strain Fitness in Pseudomonas simiae WCS417 around PS417_03370

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_03365 and PS417_03370 are separated by 344 nucleotidesPS417_03370 and PS417_03375 are separated by 225 nucleotidesPS417_03375 and PS417_03380 are separated by 38 nucleotides PS417_03365: PS417_03365 - transcriptional regulator, at 774,172 to 774,846 _03365 PS417_03370: PS417_03370 - porin, at 775,191 to 776,510 _03370 PS417_03375: PS417_03375 - hypothetical protein, at 776,736 to 777,068 _03375 PS417_03380: PS417_03380 - hypothetical protein, at 777,107 to 777,544 _03380 Position (kb) 775 776 777Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 774.557 kb on + strand, within PS417_03365at 774.557 kb on + strand, within PS417_03365at 774.557 kb on + strand, within PS417_03365at 774.558 kb on - strand, within PS417_03365at 774.767 kb on - strand, within PS417_03365at 774.767 kb on - strand, within PS417_03365at 774.774 kb on - strand, within PS417_03365at 774.803 kb on + strandat 774.840 kb on + strandat 774.854 kb on + strandat 774.855 kb on - strandat 774.855 kb on - strandat 774.909 kb on - strandat 775.043 kb on + strandat 775.122 kb on + strandat 775.123 kb on - strandat 775.157 kb on + strandat 775.158 kb on - strandat 775.178 kb on + strandat 775.178 kb on + strandat 775.178 kb on + strandat 775.321 kb on + strandat 775.321 kb on + strandat 775.322 kb on - strandat 775.393 kb on + strand, within PS417_03370at 775.393 kb on + strand, within PS417_03370at 775.394 kb on - strand, within PS417_03370at 775.414 kb on + strand, within PS417_03370at 775.537 kb on + strand, within PS417_03370at 775.537 kb on + strand, within PS417_03370at 775.582 kb on + strand, within PS417_03370at 775.583 kb on - strand, within PS417_03370at 775.700 kb on + strand, within PS417_03370at 775.700 kb on + strand, within PS417_03370at 775.700 kb on + strand, within PS417_03370at 775.700 kb on + strand, within PS417_03370at 775.701 kb on - strand, within PS417_03370at 775.720 kb on + strand, within PS417_03370at 775.720 kb on + strand, within PS417_03370at 775.721 kb on - strand, within PS417_03370at 775.721 kb on - strand, within PS417_03370at 775.723 kb on + strand, within PS417_03370at 775.724 kb on - strand, within PS417_03370at 775.724 kb on - strand, within PS417_03370at 775.724 kb on - strand, within PS417_03370at 775.786 kb on + strand, within PS417_03370at 775.786 kb on + strand, within PS417_03370at 775.903 kb on + strand, within PS417_03370at 775.903 kb on + strand, within PS417_03370at 776.076 kb on + strand, within PS417_03370at 776.076 kb on + strand, within PS417_03370at 776.140 kb on + strand, within PS417_03370at 776.140 kb on + strand, within PS417_03370at 776.141 kb on - strand, within PS417_03370at 776.141 kb on - strand, within PS417_03370at 776.141 kb on - strand, within PS417_03370at 776.494 kb on + strandat 776.494 kb on + strandat 776.626 kb on - strandat 776.662 kb on + strandat 776.663 kb on - strandat 776.817 kb on - strand, within PS417_03375at 776.817 kb on - strand, within PS417_03375at 776.817 kb on - strand, within PS417_03375at 776.840 kb on + strand, within PS417_03375at 776.994 kb on + strand, within PS417_03375at 776.995 kb on - strand, within PS417_03375at 776.995 kb on - strand, within PS417_03375at 777.020 kb on + strand, within PS417_03375at 777.031 kb on - strand, within PS417_03375at 777.252 kb on + strand, within PS417_03380at 777.454 kb on - strand, within PS417_03380

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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774,557 + PS417_03365 0.57 +0.0
774,557 + PS417_03365 0.57 -0.4
774,557 + PS417_03365 0.57 +0.4
774,558 - PS417_03365 0.57 +0.8
774,767 - PS417_03365 0.88 -1.7
774,767 - PS417_03365 0.88 +0.0
774,774 - PS417_03365 0.89 +4.2
774,803 + -0.4
774,840 + +1.5
774,854 + +1.0
774,855 - +2.9
774,855 - -0.3
774,909 - -0.4
775,043 + -0.4
775,122 + -1.1
775,123 - -1.7
775,157 + -0.8
775,158 - +0.6
775,178 + -0.2
775,178 + +1.1
775,178 + -0.9
775,321 + -2.2
775,321 + -1.7
775,322 - +0.4
775,393 + PS417_03370 0.15 +0.1
775,393 + PS417_03370 0.15 +0.3
775,394 - PS417_03370 0.15 +0.3
775,414 + PS417_03370 0.17 -0.5
775,537 + PS417_03370 0.26 +0.4
775,537 + PS417_03370 0.26 -1.3
775,582 + PS417_03370 0.30 -1.1
775,583 - PS417_03370 0.30 -0.0
775,700 + PS417_03370 0.39 -1.4
775,700 + PS417_03370 0.39 -0.4
775,700 + PS417_03370 0.39 -0.2
775,700 + PS417_03370 0.39 +0.4
775,701 - PS417_03370 0.39 -3.0
775,720 + PS417_03370 0.40 +2.0
775,720 + PS417_03370 0.40 -1.6
775,721 - PS417_03370 0.40 -0.6
775,721 - PS417_03370 0.40 -0.4
775,723 + PS417_03370 0.40 +0.9
775,724 - PS417_03370 0.40 -2.3
775,724 - PS417_03370 0.40 +1.5
775,724 - PS417_03370 0.40 +2.1
775,786 + PS417_03370 0.45 -0.5
775,786 + PS417_03370 0.45 +1.6
775,903 + PS417_03370 0.54 +0.7
775,903 + PS417_03370 0.54 +0.5
776,076 + PS417_03370 0.67 -0.2
776,076 + PS417_03370 0.67 +0.1
776,140 + PS417_03370 0.72 -0.1
776,140 + PS417_03370 0.72 +0.6
776,141 - PS417_03370 0.72 +3.8
776,141 - PS417_03370 0.72 -1.1
776,141 - PS417_03370 0.72 -0.5
776,494 + +0.7
776,494 + +0.0
776,626 - +1.0
776,662 + +0.1
776,663 - +0.1
776,817 - PS417_03375 0.24 +0.5
776,817 - PS417_03375 0.24 +1.2
776,817 - PS417_03375 0.24 -0.7
776,840 + PS417_03375 0.31 -0.3
776,994 + PS417_03375 0.77 -1.1
776,995 - PS417_03375 0.78 +1.7
776,995 - PS417_03375 0.78 -1.3
777,020 + PS417_03375 0.85 -0.1
777,031 - PS417_03375 0.89 -0.6
777,252 + PS417_03380 0.33 +1.2
777,454 - PS417_03380 0.79 +0.7

Or see this region's nucleotide sequence