Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00315 and PS417_00320 are separated by 6 nucleotides PS417_00320 and PS417_00325 are separated by 28 nucleotides PS417_00325 and PS417_00330 overlap by 35 nucleotides PS417_00330 and PS417_00335 are separated by 10 nucleotides
PS417_00315: PS417_00315 - MFS transporter, at 62,487 to 63,374
_00315
PS417_00320: PS417_00320 - hypothetical protein, at 63,381 to 63,584
_00320
PS417_00325: PS417_00325 - hypothetical protein, at 63,613 to 64,377
_00325
PS417_00330: PS417_00330 - hypothetical protein, at 64,343 to 64,927
_00330
PS417_00335: PS417_00335 - cytochrome B561, at 64,938 to 66,017
_00335
Position (kb) 64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 63.364 kb on - strand at 63.364 kb on - strand at 63.364 kb on - strand at 63.624 kb on + strand at 63.624 kb on + strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.625 kb on - strand at 63.627 kb on - strand at 63.638 kb on + strand at 63.638 kb on + strand at 63.639 kb on - strand at 63.760 kb on + strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 63.761 kb on - strand, within PS417_00325 at 64.051 kb on + strand, within PS417_00325 at 64.052 kb on - strand, within PS417_00325 at 64.052 kb on - strand, within PS417_00325 at 64.174 kb on + strand, within PS417_00325 at 64.303 kb on + strand at 64.303 kb on + strand at 64.303 kb on + strand at 64.303 kb on + strand at 64.310 kb on - strand at 64.463 kb on + strand, within PS417_00330 at 64.463 kb on + strand at 64.463 kb on + strand, within PS417_00330 at 64.464 kb on - strand, within PS417_00330 at 64.499 kb on + strand, within PS417_00330 at 64.499 kb on + strand, within PS417_00330 at 64.542 kb on + strand, within PS417_00330 at 64.543 kb on - strand, within PS417_00330 at 64.543 kb on - strand, within PS417_00330 at 64.625 kb on + strand, within PS417_00330 at 64.625 kb on + strand, within PS417_00330 at 64.716 kb on - strand, within PS417_00330 at 64.853 kb on + strand, within PS417_00330 at 64.853 kb on + strand, within PS417_00330 at 65.010 kb on + strand at 65.012 kb on + strand at 65.013 kb on - strand at 65.013 kb on - strand at 65.013 kb on - strand at 65.061 kb on + strand, within PS417_00335 at 65.062 kb on - strand, within PS417_00335 at 65.172 kb on + strand, within PS417_00335 at 65.417 kb on - strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.603 kb on + strand, within PS417_00335 at 65.604 kb on - strand, within PS417_00335 at 65.604 kb on - strand, within PS417_00335 at 65.625 kb on + strand, within PS417_00335 at 65.625 kb on + strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.626 kb on - strand, within PS417_00335 at 65.740 kb on + strand, within PS417_00335 at 65.741 kb on - strand, within PS417_00335 at 65.741 kb on - strand, within PS417_00335 at 65.745 kb on - strand, within PS417_00335 at 65.814 kb on + strand, within PS417_00335 at 65.815 kb on - strand, within PS417_00335 at 65.895 kb on + strand, within PS417_00335 at 65.896 kb on - strand, within PS417_00335 at 65.896 kb on - strand, within PS417_00335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 63,364 - +0.0 63,364 - +0.2 63,364 - -0.8 63,624 + -1.2 63,624 + -1.3 63,625 - +0.1 63,625 - +0.2 63,625 - -2.9 63,625 - -0.5 63,625 - +1.8 63,627 - +0.4 63,638 + +1.1 63,638 + -1.1 63,639 - -0.4 63,760 + PS417_00325 0.19 -3.5 63,761 - PS417_00325 0.19 +0.8 63,761 - PS417_00325 0.19 +0.5 63,761 - PS417_00325 0.19 -0.1 63,761 - PS417_00325 0.19 +0.8 64,051 + PS417_00325 0.57 +2.0 64,052 - PS417_00325 0.57 +0.3 64,052 - PS417_00325 0.57 +0.6 64,174 + PS417_00325 0.73 +0.7 64,303 + -0.4 64,303 + +0.5 64,303 + -0.8 64,303 + -0.2 64,310 - -2.3 64,463 + PS417_00330 0.21 +1.0 64,463 + +1.0 64,463 + PS417_00330 0.21 +0.3 64,464 - PS417_00330 0.21 -2.0 64,499 + PS417_00330 0.27 +2.6 64,499 + PS417_00330 0.27 +0.6 64,542 + PS417_00330 0.34 -0.7 64,543 - PS417_00330 0.34 -0.3 64,543 - PS417_00330 0.34 -0.2 64,625 + PS417_00330 0.48 -2.4 64,625 + PS417_00330 0.48 -0.4 64,716 - PS417_00330 0.64 +1.2 64,853 + PS417_00330 0.87 +0.0 64,853 + PS417_00330 0.87 -0.6 65,010 + -0.1 65,012 + -0.7 65,013 - -1.3 65,013 - -1.0 65,013 - -0.1 65,061 + PS417_00335 0.11 +2.1 65,062 - PS417_00335 0.11 -0.4 65,172 + PS417_00335 0.22 +0.0 65,417 - PS417_00335 0.44 -1.5 65,603 + PS417_00335 0.62 +0.3 65,603 + PS417_00335 0.62 -1.5 65,603 + PS417_00335 0.62 +0.6 65,603 + PS417_00335 0.62 -0.4 65,604 - PS417_00335 0.62 +1.6 65,604 - PS417_00335 0.62 +4.9 65,625 + PS417_00335 0.64 -0.5 65,625 + PS417_00335 0.64 -0.8 65,626 - PS417_00335 0.64 -0.3 65,626 - PS417_00335 0.64 -0.4 65,626 - PS417_00335 0.64 -0.1 65,740 + PS417_00335 0.74 -1.2 65,741 - PS417_00335 0.74 +0.0 65,741 - PS417_00335 0.74 -0.6 65,745 - PS417_00335 0.75 -0.8 65,814 + PS417_00335 0.81 -0.9 65,815 - PS417_00335 0.81 -2.5 65,895 + PS417_00335 0.89 +1.0 65,896 - PS417_00335 0.89 -1.6 65,896 - PS417_00335 0.89 +0.5
Or see this region's nucleotide sequence