Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26915 and PS417_26920 are separated by 5 nucleotides PS417_26920 and PS417_26925 are separated by 91 nucleotides PS417_26925 and PS417_26930 overlap by 1 nucleotides
PS417_26915: PS417_26915 - GTPase SAR1, at 5,812,696 to 5,814,063
_26915
PS417_26920: PS417_26920 - hypothetical protein, at 5,814,069 to 5,814,608
_26920
PS417_26925: PS417_26925 - phosphohydrolase, at 5,814,700 to 5,815,254
_26925
PS417_26930: PS417_26930 - FAD-dependent oxidoreductase, at 5,815,254 to 5,816,387
_26930
Position (kb) 5814
5815
5816 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5813.752 kb on + strand, within PS417_26915 at 5813.753 kb on - strand, within PS417_26915 at 5813.753 kb on - strand, within PS417_26915 at 5813.753 kb on - strand, within PS417_26915 at 5814.077 kb on + strand at 5814.138 kb on - strand, within PS417_26920 at 5814.208 kb on + strand, within PS417_26920 at 5814.209 kb on - strand, within PS417_26920 at 5814.209 kb on - strand, within PS417_26920 at 5814.224 kb on - strand, within PS417_26920 at 5814.317 kb on - strand, within PS417_26920 at 5814.329 kb on + strand, within PS417_26920 at 5814.330 kb on - strand, within PS417_26920 at 5814.330 kb on - strand, within PS417_26920 at 5814.390 kb on - strand, within PS417_26920 at 5814.453 kb on + strand, within PS417_26920 at 5814.454 kb on - strand, within PS417_26920 at 5814.454 kb on - strand, within PS417_26920 at 5814.535 kb on + strand, within PS417_26920 at 5814.569 kb on + strand at 5814.601 kb on + strand at 5814.622 kb on + strand at 5814.644 kb on - strand at 5814.690 kb on + strand at 5814.691 kb on - strand at 5814.691 kb on - strand at 5814.870 kb on - strand, within PS417_26925 at 5814.908 kb on + strand, within PS417_26925 at 5814.908 kb on + strand, within PS417_26925 at 5814.908 kb on + strand, within PS417_26925 at 5814.909 kb on - strand, within PS417_26925 at 5814.909 kb on - strand, within PS417_26925 at 5815.074 kb on + strand, within PS417_26925 at 5815.074 kb on + strand, within PS417_26925 at 5815.075 kb on - strand, within PS417_26925 at 5815.075 kb on - strand, within PS417_26925 at 5815.075 kb on - strand, within PS417_26925 at 5815.191 kb on - strand, within PS417_26925 at 5815.214 kb on + strand at 5815.214 kb on + strand at 5815.215 kb on - strand at 5815.215 kb on - strand at 5815.215 kb on - strand at 5815.280 kb on + strand at 5815.281 kb on - strand at 5815.281 kb on - strand at 5815.397 kb on - strand, within PS417_26930 at 5815.511 kb on - strand, within PS417_26930 at 5815.528 kb on - strand, within PS417_26930 at 5815.885 kb on + strand, within PS417_26930 at 5815.885 kb on + strand, within PS417_26930 at 5815.885 kb on + strand, within PS417_26930 at 5815.885 kb on + strand, within PS417_26930 at 5815.886 kb on - strand, within PS417_26930 at 5815.915 kb on + strand, within PS417_26930 at 5816.008 kb on + strand, within PS417_26930 at 5816.050 kb on + strand, within PS417_26930 at 5816.126 kb on - strand, within PS417_26930
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,813,752 + PS417_26915 0.77 -0.0 5,813,753 - PS417_26915 0.77 +0.0 5,813,753 - PS417_26915 0.77 -1.7 5,813,753 - PS417_26915 0.77 -1.8 5,814,077 + -0.0 5,814,138 - PS417_26920 0.13 -1.3 5,814,208 + PS417_26920 0.26 -0.4 5,814,209 - PS417_26920 0.26 +0.1 5,814,209 - PS417_26920 0.26 -0.0 5,814,224 - PS417_26920 0.29 +0.3 5,814,317 - PS417_26920 0.46 -0.2 5,814,329 + PS417_26920 0.48 +0.0 5,814,330 - PS417_26920 0.48 -0.4 5,814,330 - PS417_26920 0.48 +0.9 5,814,390 - PS417_26920 0.59 -0.2 5,814,453 + PS417_26920 0.71 -0.8 5,814,454 - PS417_26920 0.71 -1.0 5,814,454 - PS417_26920 0.71 +0.4 5,814,535 + PS417_26920 0.86 -1.2 5,814,569 + -0.4 5,814,601 + +0.7 5,814,622 + -0.9 5,814,644 - +0.7 5,814,690 + -0.2 5,814,691 - -0.5 5,814,691 - -0.9 5,814,870 - PS417_26925 0.31 -1.3 5,814,908 + PS417_26925 0.37 +0.1 5,814,908 + PS417_26925 0.37 +0.2 5,814,908 + PS417_26925 0.37 -0.3 5,814,909 - PS417_26925 0.38 -0.2 5,814,909 - PS417_26925 0.38 +2.1 5,815,074 + PS417_26925 0.67 +1.6 5,815,074 + PS417_26925 0.67 +0.4 5,815,075 - PS417_26925 0.68 +0.5 5,815,075 - PS417_26925 0.68 -0.5 5,815,075 - PS417_26925 0.68 -0.2 5,815,191 - PS417_26925 0.88 -2.0 5,815,214 + -1.0 5,815,214 + -0.0 5,815,215 - +0.7 5,815,215 - +0.6 5,815,215 - -1.2 5,815,280 + +0.3 5,815,281 - -0.7 5,815,281 - +1.5 5,815,397 - PS417_26930 0.13 -1.6 5,815,511 - PS417_26930 0.23 -1.3 5,815,528 - PS417_26930 0.24 -1.4 5,815,885 + PS417_26930 0.56 -0.9 5,815,885 + PS417_26930 0.56 +0.8 5,815,885 + PS417_26930 0.56 -2.3 5,815,885 + PS417_26930 0.56 -3.4 5,815,886 - PS417_26930 0.56 -0.3 5,815,915 + PS417_26930 0.58 -0.3 5,816,008 + PS417_26930 0.66 -1.5 5,816,050 + PS417_26930 0.70 -1.0 5,816,126 - PS417_26930 0.77 -3.4
Or see this region's nucleotide sequence