Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26270 and PS417_26275 are separated by 207 nucleotides PS417_26275 and PS417_26280 are separated by 73 nucleotides
PS417_26270: PS417_26270 - AraC family transcriptional regulator, at 5,677,248 to 5,678,192
_26270
PS417_26275: PS417_26275 - glycine/betaine ABC transporter substrate-binding protein, at 5,678,400 to 5,679,344
_26275
PS417_26280: PS417_26280 - NADPH:quinone reductase, at 5,679,418 to 5,680,305
_26280
Position (kb)
5678
5679
5680 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5677.417 kb on + strand, within PS417_26270 at 5677.417 kb on + strand, within PS417_26270 at 5678.139 kb on - strand at 5678.177 kb on - strand at 5678.231 kb on + strand at 5678.317 kb on + strand at 5678.318 kb on - strand at 5678.318 kb on - strand at 5678.322 kb on - strand at 5678.326 kb on + strand at 5678.326 kb on + strand at 5678.464 kb on + strand at 5678.527 kb on + strand, within PS417_26275 at 5678.769 kb on + strand, within PS417_26275 at 5678.769 kb on + strand, within PS417_26275 at 5678.770 kb on - strand, within PS417_26275 at 5678.770 kb on - strand, within PS417_26275 at 5679.342 kb on + strand at 5679.343 kb on - strand at 5679.396 kb on + strand at 5679.397 kb on - strand at 5679.841 kb on + strand, within PS417_26280 at 5679.841 kb on + strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.842 kb on - strand, within PS417_26280 at 5679.850 kb on - strand, within PS417_26280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,677,417 + PS417_26270 0.18 -1.2 5,677,417 + PS417_26270 0.18 +4.4 5,678,139 - -1.8 5,678,177 - -0.8 5,678,231 + +0.1 5,678,317 + -0.5 5,678,318 - +0.4 5,678,318 - +0.6 5,678,322 - -0.0 5,678,326 + -1.5 5,678,326 + -0.1 5,678,464 + +1.2 5,678,527 + PS417_26275 0.13 +0.6 5,678,769 + PS417_26275 0.39 +0.5 5,678,769 + PS417_26275 0.39 +1.2 5,678,770 - PS417_26275 0.39 +2.1 5,678,770 - PS417_26275 0.39 -0.6 5,679,342 + -1.8 5,679,343 - +3.5 5,679,396 + -1.3 5,679,397 - +0.4 5,679,841 + PS417_26280 0.48 -0.5 5,679,841 + PS417_26280 0.48 -0.3 5,679,842 - PS417_26280 0.48 +0.5 5,679,842 - PS417_26280 0.48 -0.4 5,679,850 - PS417_26280 0.49 -1.8
Or see this region's nucleotide sequence