Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24955 and PS417_24960 are separated by 112 nucleotides PS417_24960 and PS417_24965 are separated by 81 nucleotides
PS417_24955: PS417_24955 - hypothetical protein, at 5,411,756 to 5,411,992
_24955
PS417_24960: PS417_24960 - transposase, at 5,412,105 to 5,413,682
_24960
PS417_24965: PS417_24965 - transposase, at 5,413,764 to 5,414,099
_24965
Position (kb) 5412
5413
5414 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5411.105 kb on - strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.108 kb on + strand at 5411.109 kb on - strand at 5411.109 kb on - strand at 5411.125 kb on + strand at 5411.179 kb on + strand at 5411.218 kb on + strand at 5411.219 kb on - strand at 5411.219 kb on - strand at 5411.238 kb on + strand at 5411.238 kb on + strand at 5411.238 kb on + strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.239 kb on - strand at 5411.368 kb on + strand at 5411.378 kb on + strand at 5411.378 kb on + strand at 5411.379 kb on - strand at 5411.379 kb on - strand at 5411.408 kb on + strand at 5411.423 kb on + strand at 5411.445 kb on - strand at 5411.445 kb on - strand at 5411.480 kb on + strand at 5411.480 kb on + strand at 5411.535 kb on - strand at 5411.575 kb on + strand at 5411.575 kb on + strand at 5411.576 kb on - strand at 5411.576 kb on - strand at 5411.577 kb on + strand at 5411.577 kb on + strand at 5411.578 kb on - strand at 5411.578 kb on - strand at 5411.578 kb on - strand at 5411.578 kb on - strand at 5411.578 kb on - strand at 5411.633 kb on + strand at 5411.633 kb on + strand at 5411.634 kb on - strand at 5411.663 kb on + strand at 5411.663 kb on + strand at 5411.664 kb on - strand at 5411.683 kb on + strand at 5411.684 kb on - strand at 5411.725 kb on + strand at 5411.726 kb on - strand at 5411.726 kb on - strand at 5411.739 kb on + strand at 5411.740 kb on - strand at 5411.763 kb on + strand at 5411.764 kb on - strand at 5411.764 kb on - strand at 5411.797 kb on + strand, within PS417_24955 at 5411.797 kb on + strand, within PS417_24955 at 5411.798 kb on - strand, within PS417_24955 at 5411.802 kb on + strand, within PS417_24955 at 5411.802 kb on + strand, within PS417_24955 at 5411.803 kb on - strand, within PS417_24955 at 5411.803 kb on - strand, within PS417_24955 at 5411.803 kb on - strand, within PS417_24955 at 5411.803 kb on - strand, within PS417_24955 at 5411.803 kb on - strand, within PS417_24955 at 5411.803 kb on - strand at 5411.803 kb on - strand, within PS417_24955 at 5411.855 kb on + strand, within PS417_24955 at 5411.855 kb on + strand, within PS417_24955 at 5411.855 kb on + strand, within PS417_24955 at 5411.856 kb on - strand, within PS417_24955 at 5411.860 kb on + strand, within PS417_24955 at 5411.860 kb on + strand, within PS417_24955 at 5411.860 kb on + strand, within PS417_24955 at 5411.860 kb on + strand at 5411.860 kb on + strand, within PS417_24955 at 5411.860 kb on + strand, within PS417_24955 at 5411.861 kb on - strand, within PS417_24955 at 5411.861 kb on - strand, within PS417_24955 at 5411.861 kb on - strand, within PS417_24955 at 5411.861 kb on - strand, within PS417_24955 at 5411.991 kb on - strand at 5412.073 kb on + strand at 5412.073 kb on + strand at 5412.074 kb on - strand at 5412.091 kb on + strand at 5412.106 kb on + strand at 5412.107 kb on - strand at 5412.117 kb on + strand at 5412.118 kb on - strand at 5412.186 kb on + strand at 5412.186 kb on + strand at 5412.187 kb on - strand at 5412.187 kb on - strand at 5412.199 kb on + strand at 5412.199 kb on + strand at 5412.200 kb on - strand at 5412.200 kb on - strand at 5412.205 kb on + strand at 5412.217 kb on - strand at 5412.325 kb on - strand, within PS417_24960 at 5412.473 kb on + strand, within PS417_24960 at 5412.558 kb on + strand, within PS417_24960 at 5412.566 kb on - strand, within PS417_24960 at 5412.580 kb on + strand, within PS417_24960 at 5412.580 kb on + strand, within PS417_24960 at 5412.581 kb on - strand, within PS417_24960 at 5412.695 kb on + strand, within PS417_24960 at 5412.695 kb on + strand, within PS417_24960 at 5412.696 kb on - strand, within PS417_24960 at 5412.696 kb on - strand, within PS417_24960 at 5412.696 kb on - strand, within PS417_24960 at 5412.696 kb on - strand, within PS417_24960 at 5412.697 kb on + strand, within PS417_24960 at 5412.698 kb on - strand, within PS417_24960 at 5412.698 kb on - strand, within PS417_24960 at 5412.717 kb on + strand, within PS417_24960 at 5412.718 kb on - strand, within PS417_24960 at 5412.788 kb on - strand, within PS417_24960 at 5412.788 kb on - strand, within PS417_24960 at 5412.788 kb on - strand, within PS417_24960 at 5412.788 kb on - strand, within PS417_24960 at 5412.838 kb on + strand, within PS417_24960 at 5412.838 kb on + strand, within PS417_24960 at 5412.900 kb on + strand, within PS417_24960 at 5412.901 kb on - strand, within PS417_24960 at 5412.901 kb on - strand, within PS417_24960 at 5412.918 kb on + strand, within PS417_24960 at 5413.060 kb on + strand, within PS417_24960 at 5413.061 kb on - strand, within PS417_24960 at 5413.061 kb on - strand, within PS417_24960 at 5413.103 kb on + strand, within PS417_24960 at 5413.104 kb on - strand, within PS417_24960 at 5413.181 kb on + strand, within PS417_24960 at 5413.213 kb on + strand, within PS417_24960 at 5413.337 kb on + strand, within PS417_24960 at 5413.338 kb on - strand, within PS417_24960 at 5413.550 kb on + strand at 5413.654 kb on - strand at 5413.654 kb on - strand at 5413.736 kb on + strand at 5413.981 kb on + strand at 5413.981 kb on + strand, within PS417_24965 at 5413.981 kb on + strand at 5413.982 kb on - strand at 5413.982 kb on - strand, within PS417_24965 at 5413.982 kb on - strand, within PS417_24965 at 5414.157 kb on - strand at 5414.178 kb on + strand at 5414.178 kb on + strand at 5414.184 kb on - strand at 5414.213 kb on + strand at 5414.213 kb on + strand at 5414.237 kb on + strand at 5414.237 kb on + strand at 5414.237 kb on + strand at 5414.237 kb on + strand at 5414.400 kb on + strand at 5414.400 kb on + strand at 5414.401 kb on - strand at 5414.401 kb on - strand at 5414.455 kb on - strand at 5414.494 kb on + strand at 5414.526 kb on + strand at 5414.584 kb on + strand at 5414.584 kb on + strand at 5414.585 kb on - strand at 5414.585 kb on - strand at 5414.612 kb on + strand at 5414.612 kb on + strand at 5414.613 kb on - strand at 5414.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,411,105 - -0.6 5,411,108 + +2.8 5,411,108 + -0.2 5,411,108 + -1.6 5,411,108 + +0.3 5,411,108 + +1.1 5,411,108 + -1.0 5,411,108 + +0.2 5,411,108 + +0.7 5,411,109 - -1.7 5,411,109 - -1.5 5,411,125 + -0.2 5,411,179 + +0.6 5,411,218 + -0.3 5,411,219 - -1.1 5,411,219 - -1.0 5,411,238 + -0.8 5,411,238 + -0.4 5,411,238 + +0.1 5,411,239 - -0.7 5,411,239 - +0.8 5,411,239 - +0.8 5,411,239 - -1.8 5,411,239 - -0.7 5,411,239 - -0.7 5,411,239 - +0.9 5,411,239 - -2.0 5,411,368 + +1.1 5,411,378 + -2.6 5,411,378 + +0.1 5,411,379 - -0.5 5,411,379 - +0.2 5,411,408 + +0.3 5,411,423 + -0.5 5,411,445 - -0.5 5,411,445 - +1.0 5,411,480 + +2.1 5,411,480 + -3.1 5,411,535 - -3.2 5,411,575 + +0.1 5,411,575 + -1.7 5,411,576 - -3.7 5,411,576 - -0.6 5,411,577 + -0.3 5,411,577 + +0.6 5,411,578 - +1.3 5,411,578 - -0.0 5,411,578 - -1.7 5,411,578 - -1.1 5,411,578 - +0.3 5,411,633 + +0.3 5,411,633 + +0.3 5,411,634 - -0.7 5,411,663 + -1.4 5,411,663 + -0.9 5,411,664 - +0.5 5,411,683 + -0.1 5,411,684 - +1.1 5,411,725 + -0.0 5,411,726 - +3.2 5,411,726 - +0.0 5,411,739 + -0.4 5,411,740 - -0.4 5,411,763 + -1.7 5,411,764 - -2.2 5,411,764 - +0.3 5,411,797 + PS417_24955 0.17 -0.2 5,411,797 + PS417_24955 0.17 -2.3 5,411,798 - PS417_24955 0.18 -0.2 5,411,802 + PS417_24955 0.19 -0.3 5,411,802 + PS417_24955 0.19 -1.7 5,411,803 - PS417_24955 0.20 -0.5 5,411,803 - PS417_24955 0.20 +0.1 5,411,803 - PS417_24955 0.20 -0.2 5,411,803 - PS417_24955 0.20 -1.2 5,411,803 - PS417_24955 0.20 -2.0 5,411,803 - -1.2 5,411,803 - PS417_24955 0.20 -0.2 5,411,855 + PS417_24955 0.42 -0.6 5,411,855 + PS417_24955 0.42 -0.0 5,411,855 + PS417_24955 0.42 +0.2 5,411,856 - PS417_24955 0.42 +0.6 5,411,860 + PS417_24955 0.44 -0.3 5,411,860 + PS417_24955 0.44 +1.0 5,411,860 + PS417_24955 0.44 -0.7 5,411,860 + +0.6 5,411,860 + PS417_24955 0.44 -1.0 5,411,860 + PS417_24955 0.44 -1.2 5,411,861 - PS417_24955 0.44 -3.1 5,411,861 - PS417_24955 0.44 -0.4 5,411,861 - PS417_24955 0.44 +1.3 5,411,861 - PS417_24955 0.44 -1.6 5,411,991 - +0.4 5,412,073 + +1.3 5,412,073 + -1.4 5,412,074 - -1.1 5,412,091 + +0.0 5,412,106 + -0.2 5,412,107 - +1.9 5,412,117 + -0.0 5,412,118 - -1.5 5,412,186 + +1.4 5,412,186 + -0.0 5,412,187 - -2.2 5,412,187 - -0.2 5,412,199 + -0.3 5,412,199 + -0.3 5,412,200 - -0.7 5,412,200 - +0.2 5,412,205 + -0.0 5,412,217 - +0.2 5,412,325 - PS417_24960 0.14 -2.8 5,412,473 + PS417_24960 0.23 +0.3 5,412,558 + PS417_24960 0.29 -1.1 5,412,566 - PS417_24960 0.29 -0.5 5,412,580 + PS417_24960 0.30 +0.6 5,412,580 + PS417_24960 0.30 -0.2 5,412,581 - PS417_24960 0.30 -1.4 5,412,695 + PS417_24960 0.37 +0.5 5,412,695 + PS417_24960 0.37 -0.4 5,412,696 - PS417_24960 0.37 -0.3 5,412,696 - PS417_24960 0.37 +1.0 5,412,696 - PS417_24960 0.37 +0.8 5,412,696 - PS417_24960 0.37 +0.0 5,412,697 + PS417_24960 0.38 +0.8 5,412,698 - PS417_24960 0.38 +0.7 5,412,698 - PS417_24960 0.38 +1.5 5,412,717 + PS417_24960 0.39 -1.0 5,412,718 - PS417_24960 0.39 -0.0 5,412,788 - PS417_24960 0.43 +0.4 5,412,788 - PS417_24960 0.43 +0.8 5,412,788 - PS417_24960 0.43 -0.2 5,412,788 - PS417_24960 0.43 +2.2 5,412,838 + PS417_24960 0.46 +0.8 5,412,838 + PS417_24960 0.46 -0.0 5,412,900 + PS417_24960 0.50 +0.2 5,412,901 - PS417_24960 0.50 +0.5 5,412,901 - PS417_24960 0.50 -0.4 5,412,918 + PS417_24960 0.52 -0.3 5,413,060 + PS417_24960 0.61 +0.5 5,413,061 - PS417_24960 0.61 +0.7 5,413,061 - PS417_24960 0.61 +1.5 5,413,103 + PS417_24960 0.63 -1.1 5,413,104 - PS417_24960 0.63 -0.9 5,413,181 + PS417_24960 0.68 -0.9 5,413,213 + PS417_24960 0.70 +0.9 5,413,337 + PS417_24960 0.78 +3.4 5,413,338 - PS417_24960 0.78 +1.6 5,413,550 + -0.2 5,413,654 - -1.9 5,413,654 - +0.2 5,413,736 + -4.3 5,413,981 + -0.7 5,413,981 + PS417_24965 0.65 +4.4 5,413,981 + +0.6 5,413,982 - -0.2 5,413,982 - PS417_24965 0.65 -2.1 5,413,982 - PS417_24965 0.65 -1.6 5,414,157 - -0.6 5,414,178 + -0.1 5,414,178 + -2.4 5,414,184 - -0.2 5,414,213 + +0.1 5,414,213 + +2.5 5,414,237 + -0.5 5,414,237 + -0.0 5,414,237 + +1.1 5,414,237 + -1.6 5,414,400 + -0.7 5,414,400 + +2.3 5,414,401 - +0.2 5,414,401 - +1.2 5,414,455 - -0.0 5,414,494 + -1.3 5,414,526 + -0.1 5,414,584 + -1.3 5,414,584 + +0.5 5,414,585 - -0.6 5,414,585 - +0.2 5,414,612 + +0.3 5,414,612 + +0.0 5,414,613 - -0.1 5,414,613 - +0.2
Or see this region's nucleotide sequence