Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02450 and PS417_02455 are separated by 194 nucleotides PS417_02455 and PS417_02460 overlap by 4 nucleotides PS417_02460 and PS417_02465 are separated by 63 nucleotides PS417_02465 and PS417_02470 overlap by 13 nucleotides
PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810
_02450
PS417_02455: PS417_02455 - putative transporter, required for glycine utilization <small>(from data)</small>, at 562,005 to 562,619
_02455
PS417_02460: PS417_02460 - epoxyqueuosine reductase, at 562,616 to 563,680
_02460
PS417_02465: PS417_02465 - carbohydrate kinase, at 563,744 to 564,604
_02465
PS417_02470: PS417_02470 - ATPase, at 564,592 to 565,062
_02470
Position (kb) 562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 562.020 kb on + strand at 562.020 kb on + strand at 562.447 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.494 kb on + strand, within PS417_02455 at 562.600 kb on - strand at 562.618 kb on - strand at 562.828 kb on + strand, within PS417_02460 at 562.828 kb on + strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 562.829 kb on - strand, within PS417_02460 at 563.041 kb on - strand, within PS417_02460 at 563.167 kb on - strand, within PS417_02460 at 563.190 kb on - strand, within PS417_02460 at 563.379 kb on + strand, within PS417_02460 at 563.380 kb on - strand, within PS417_02460 at 563.402 kb on + strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.434 kb on - strand, within PS417_02460 at 563.517 kb on + strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.518 kb on - strand, within PS417_02460 at 563.713 kb on - strand at 563.775 kb on + strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.862 kb on + strand, within PS417_02465 at 563.863 kb on - strand, within PS417_02465 at 564.275 kb on - strand, within PS417_02465 at 564.465 kb on - strand, within PS417_02465 at 564.555 kb on + strand at 564.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 562,020 + +0.7 562,020 + -0.2 562,447 - PS417_02455 0.72 -1.8 562,480 - PS417_02455 0.77 -0.1 562,480 - PS417_02455 0.77 +1.8 562,482 - PS417_02455 0.78 +0.3 562,482 - PS417_02455 0.78 +0.9 562,494 + PS417_02455 0.80 +0.3 562,600 - +2.0 562,618 - -1.8 562,828 + PS417_02460 0.20 -0.0 562,828 + PS417_02460 0.20 -0.9 562,829 - PS417_02460 0.20 -1.8 562,829 - PS417_02460 0.20 -1.0 562,829 - PS417_02460 0.20 -0.9 563,041 - PS417_02460 0.40 -0.7 563,167 - PS417_02460 0.52 +0.6 563,190 - PS417_02460 0.54 -0.7 563,379 + PS417_02460 0.72 -1.3 563,380 - PS417_02460 0.72 -1.9 563,402 + PS417_02460 0.74 -0.1 563,434 - PS417_02460 0.77 +2.0 563,434 - PS417_02460 0.77 +0.7 563,517 + PS417_02460 0.85 +0.6 563,518 - PS417_02460 0.85 -0.5 563,518 - PS417_02460 0.85 -0.1 563,518 - PS417_02460 0.85 -3.2 563,713 - +3.3 563,775 + +0.8 563,776 - +1.4 563,776 - -0.2 563,776 - -0.5 563,776 - -0.4 563,776 - +2.1 563,776 - +0.1 563,862 + PS417_02465 0.14 -0.3 563,862 + PS417_02465 0.14 -2.0 563,862 + PS417_02465 0.14 +0.0 563,862 + PS417_02465 0.14 -1.2 563,863 - PS417_02465 0.14 -2.1 564,275 - PS417_02465 0.62 +0.0 564,465 - PS417_02465 0.84 -1.8 564,555 + -0.7 564,602 + -0.4
Or see this region's nucleotide sequence