Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24415 and PS417_24420 are separated by 63 nucleotides PS417_24420 and PS417_24430 are separated by 206 nucleotides PS417_24430 and PS417_24435 overlap by 4 nucleotides PS417_24435 and PS417_24440 are separated by 133 nucleotides
PS417_24415: PS417_24415 - siderophore-interacting protein, at 5,302,120 to 5,303,118
_24415
PS417_24420: PS417_24420 - RNA polymerase sigma70, at 5,303,182 to 5,303,703
_24420
PS417_24430: PS417_24430 - 3-dehydroquinate dehydratase, at 5,303,910 to 5,304,359
_24430
PS417_24435: PS417_24435 - shikimate dehydrogenase, at 5,304,356 to 5,305,204
_24435
PS417_24440: PS417_24440 - TetR family transcriptional regulator, at 5,305,338 to 5,305,979
_24440
Position (kb) 5303
5304
5305 Strain fitness (log2 ratio) -2
-1
0
1 at 5302.932 kb on - strand, within PS417_24415 at 5303.086 kb on - strand at 5303.141 kb on + strand at 5303.142 kb on - strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.145 kb on + strand at 5303.146 kb on - strand at 5303.231 kb on + strand at 5303.232 kb on - strand at 5303.529 kb on - strand, within PS417_24420 at 5303.743 kb on + strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.892 kb on - strand at 5303.980 kb on - strand, within PS417_24430 at 5304.340 kb on + strand at 5304.341 kb on - strand at 5304.558 kb on + strand, within PS417_24435 at 5304.559 kb on + strand, within PS417_24435 at 5304.563 kb on + strand, within PS417_24435 at 5304.817 kb on + strand, within PS417_24435 at 5304.818 kb on - strand, within PS417_24435 at 5305.034 kb on + strand, within PS417_24435 at 5305.198 kb on + strand at 5305.198 kb on + strand at 5305.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,302,932 - PS417_24415 0.81 -1.1 5,303,086 - -1.4 5,303,141 + -2.3 5,303,142 - -0.1 5,303,145 + -2.2 5,303,145 + +0.9 5,303,145 + +0.1 5,303,146 - -0.4 5,303,231 + -0.3 5,303,232 - -1.0 5,303,529 - PS417_24420 0.66 -2.0 5,303,743 + -0.7 5,303,892 - +1.1 5,303,892 - -0.2 5,303,892 - +0.3 5,303,892 - +0.6 5,303,892 - -0.6 5,303,980 - PS417_24430 0.16 -1.4 5,304,340 + -0.9 5,304,341 - +1.2 5,304,558 + PS417_24435 0.24 +0.5 5,304,559 + PS417_24435 0.24 -0.1 5,304,563 + PS417_24435 0.24 -0.7 5,304,817 + PS417_24435 0.54 -0.1 5,304,818 - PS417_24435 0.54 -0.6 5,305,034 + PS417_24435 0.80 -1.5 5,305,198 + +0.3 5,305,198 + +0.6 5,305,335 + -2.2
Or see this region's nucleotide sequence