Strain Fitness in Pseudomonas simiae WCS417 around PS417_02405

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02400 and PS417_02405 are separated by 173 nucleotidesPS417_02405 and PS417_02410 are separated by 175 nucleotides PS417_02400: PS417_02400 - DNA topoisomerase IV subunit A, at 548,450 to 550,714 _02400 PS417_02405: PS417_02405 - hypothetical protein, at 550,888 to 551,601 _02405 PS417_02410: PS417_02410 - histidine kinase, at 551,777 to 553,279 _02410 Position (kb) 550 551 552Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 550.797 kb on + strandat 550.833 kb on + strandat 550.844 kb on + strandat 550.850 kb on + strandat 550.850 kb on + strandat 550.850 kb on + strandat 550.850 kb on + strandat 550.851 kb on - strandat 550.978 kb on + strand, within PS417_02405at 550.979 kb on - strand, within PS417_02405at 551.236 kb on - strand, within PS417_02405at 551.266 kb on - strand, within PS417_02405at 551.343 kb on - strand, within PS417_02405at 551.712 kb on + strandat 551.712 kb on + strandat 551.713 kb on - strandat 551.730 kb on + strandat 551.763 kb on + strandat 551.763 kb on + strandat 551.764 kb on - strandat 551.764 kb on - strandat 551.770 kb on + strandat 551.770 kb on + strandat 551.771 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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550,797 + +0.1
550,833 + -0.6
550,844 + -0.5
550,850 + +0.7
550,850 + -0.4
550,850 + +0.2
550,850 + -1.4
550,851 - +5.1
550,978 + PS417_02405 0.13 -1.3
550,979 - PS417_02405 0.13 -1.2
551,236 - PS417_02405 0.49 -0.4
551,266 - PS417_02405 0.53 +0.6
551,343 - PS417_02405 0.64 -0.3
551,712 + +0.4
551,712 + +0.2
551,713 - -1.6
551,730 + -0.0
551,763 + -0.4
551,763 + -1.4
551,764 - +0.2
551,764 - -0.9
551,770 + -1.6
551,770 + -0.0
551,771 - -2.1

Or see this region's nucleotide sequence