Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23810 and PS417_23815 are separated by 431 nucleotides PS417_23815 and PS417_23820 overlap by 1 nucleotides PS417_23820 and PS417_23825 are separated by 16 nucleotides
PS417_23810: PS417_23810 - acetolactate synthase 3 catalytic subunit, at 5,151,863 to 5,153,587
_23810
PS417_23815: PS417_23815 - pseudouridine synthase, at 5,154,019 to 5,154,348
_23815
PS417_23820: PS417_23820 - hypothetical protein, at 5,154,348 to 5,155,082
_23820
PS417_23825: PS417_23825 - penicillin-binding protein, at 5,155,099 to 5,157,423
_23825
Position (kb) 5154
5155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5153.067 kb on + strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.149 kb on - strand, within PS417_23810 at 5153.228 kb on - strand, within PS417_23810 at 5153.228 kb on - strand, within PS417_23810 at 5153.319 kb on + strand, within PS417_23810 at 5153.319 kb on + strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.487 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.521 kb on - strand at 5153.524 kb on + strand at 5153.526 kb on + strand at 5153.526 kb on + strand at 5153.527 kb on - strand at 5153.527 kb on - strand at 5153.577 kb on - strand at 5153.600 kb on + strand at 5153.613 kb on + strand at 5153.613 kb on + strand at 5153.640 kb on + strand at 5153.641 kb on - strand at 5153.643 kb on + strand at 5153.643 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.687 kb on + strand at 5153.775 kb on - strand at 5153.775 kb on - strand at 5154.185 kb on - strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.528 kb on + strand, within PS417_23820 at 5154.528 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.870 kb on + strand, within PS417_23820 at 5155.055 kb on + strand at 5155.058 kb on + strand at 5155.059 kb on - strand at 5155.059 kb on - strand at 5155.199 kb on + strand at 5155.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,153,067 + PS417_23810 0.70 -1.8 5,153,068 - PS417_23810 0.70 +0.3 5,153,068 - PS417_23810 0.70 +0.7 5,153,068 - PS417_23810 0.70 -0.6 5,153,149 - PS417_23810 0.75 +0.3 5,153,228 - PS417_23810 0.79 -0.5 5,153,228 - PS417_23810 0.79 +3.8 5,153,319 + PS417_23810 0.84 -1.4 5,153,319 + PS417_23810 0.84 -2.1 5,153,320 - PS417_23810 0.84 -1.4 5,153,320 - PS417_23810 0.84 -0.8 5,153,320 - PS417_23810 0.84 +2.3 5,153,320 - PS417_23810 0.84 +2.7 5,153,487 + +0.4 5,153,520 + +0.9 5,153,520 + -1.2 5,153,520 + +0.7 5,153,521 - -0.6 5,153,524 + -0.3 5,153,526 + -1.9 5,153,526 + -0.9 5,153,527 - -0.9 5,153,527 - -2.2 5,153,577 - -0.6 5,153,600 + -0.9 5,153,613 + +1.3 5,153,613 + -0.6 5,153,640 + +1.3 5,153,641 - +0.5 5,153,643 + +1.5 5,153,643 + -0.4 5,153,649 + +1.6 5,153,649 + -1.6 5,153,649 + -0.4 5,153,687 + -1.2 5,153,775 - +4.1 5,153,775 - -0.5 5,154,185 - PS417_23815 0.50 +0.6 5,154,313 + PS417_23815 0.89 +1.5 5,154,313 + PS417_23815 0.89 -2.0 5,154,313 + PS417_23815 0.89 +0.5 5,154,313 + PS417_23815 0.89 +1.1 5,154,314 - PS417_23815 0.89 +0.2 5,154,314 - PS417_23815 0.89 -1.1 5,154,314 - PS417_23815 0.89 +0.6 5,154,314 - PS417_23815 0.89 +0.3 5,154,528 + PS417_23820 0.24 +0.3 5,154,528 + PS417_23820 0.24 -1.4 5,154,742 + PS417_23820 0.54 -1.2 5,154,742 + PS417_23820 0.54 -1.7 5,154,742 + PS417_23820 0.54 -0.5 5,154,743 - PS417_23820 0.54 -1.6 5,154,743 - PS417_23820 0.54 +1.3 5,154,743 - PS417_23820 0.54 -1.8 5,154,743 - PS417_23820 0.54 -0.5 5,154,870 + PS417_23820 0.71 -1.9 5,155,055 + +0.0 5,155,058 + -0.7 5,155,059 - -3.1 5,155,059 - -1.6 5,155,199 + -1.0 5,155,200 - +0.1
Or see this region's nucleotide sequence