Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23625 and PS417_23630 are separated by 30 nucleotides PS417_23630 and PS417_23635 are separated by 120 nucleotides PS417_23635 and PS417_23640 are separated by 123 nucleotides
PS417_23625: PS417_23625 - LysR family transcriptional regulator, at 5,109,708 to 5,110,604
_23625
PS417_23630: PS417_23630 - NADPH quinone reductase MdaB, at 5,110,635 to 5,111,225
_23630
PS417_23635: PS417_23635 - membrane protein, at 5,111,346 to 5,112,110
_23635
PS417_23640: PS417_23640 - LysR family transcriptional regulator, at 5,112,234 to 5,113,124
_23640
PS417_23645: PS417_23645 - amino acid permease, at 5,113,070 to 5,114,476
_23645
Position (kb)
5111
5112
5113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5110.581 kb on + strand at 5110.581 kb on + strand at 5110.582 kb on - strand at 5110.636 kb on + strand at 5110.636 kb on + strand at 5110.783 kb on + strand, within PS417_23630 at 5110.783 kb on + strand, within PS417_23630 at 5110.783 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.871 kb on - strand, within PS417_23630 at 5110.871 kb on - strand, within PS417_23630 at 5110.903 kb on + strand, within PS417_23630 at 5110.903 kb on + strand, within PS417_23630 at 5110.984 kb on + strand, within PS417_23630 at 5111.032 kb on + strand, within PS417_23630 at 5111.032 kb on + strand, within PS417_23630 at 5111.033 kb on - strand, within PS417_23630 at 5111.083 kb on + strand, within PS417_23630 at 5111.167 kb on + strand at 5111.309 kb on + strand at 5111.715 kb on + strand, within PS417_23635 at 5111.716 kb on - strand, within PS417_23635 at 5111.716 kb on - strand, within PS417_23635 at 5111.752 kb on + strand, within PS417_23635 at 5111.752 kb on + strand, within PS417_23635 at 5111.753 kb on - strand, within PS417_23635 at 5111.804 kb on + strand, within PS417_23635 at 5111.966 kb on + strand, within PS417_23635 at 5111.966 kb on + strand, within PS417_23635 at 5111.966 kb on + strand, within PS417_23635 at 5111.966 kb on + strand, within PS417_23635 at 5111.967 kb on - strand, within PS417_23635 at 5111.967 kb on - strand, within PS417_23635 at 5112.089 kb on - strand at 5112.104 kb on + strand at 5112.104 kb on + strand at 5112.105 kb on - strand at 5112.105 kb on - strand at 5112.153 kb on + strand at 5112.153 kb on + strand at 5112.155 kb on + strand at 5112.155 kb on + strand at 5112.156 kb on - strand at 5112.156 kb on - strand at 5112.156 kb on - strand at 5112.189 kb on + strand at 5112.293 kb on + strand at 5112.293 kb on + strand at 5112.294 kb on - strand at 5112.319 kb on + strand at 5112.320 kb on - strand at 5112.580 kb on + strand, within PS417_23640 at 5112.795 kb on + strand, within PS417_23640 at 5112.795 kb on + strand, within PS417_23640 at 5112.837 kb on + strand at 5112.838 kb on - strand, within PS417_23640 at 5112.886 kb on + strand, within PS417_23640 at 5112.886 kb on + strand, within PS417_23640 at 5112.887 kb on - strand, within PS417_23640 at 5112.887 kb on - strand, within PS417_23640 at 5112.887 kb on - strand, within PS417_23640
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,110,581 + -3.0 5,110,581 + -0.2 5,110,582 - +0.4 5,110,636 + +1.6 5,110,636 + +0.3 5,110,783 + PS417_23630 0.25 +0.0 5,110,783 + PS417_23630 0.25 +2.7 5,110,783 + PS417_23630 0.25 -1.4 5,110,870 + PS417_23630 0.40 +2.6 5,110,870 + PS417_23630 0.40 -1.0 5,110,870 + PS417_23630 0.40 +0.1 5,110,870 + PS417_23630 0.40 +0.9 5,110,870 + PS417_23630 0.40 -0.5 5,110,871 - PS417_23630 0.40 -0.3 5,110,871 - PS417_23630 0.40 -0.2 5,110,903 + PS417_23630 0.45 +1.0 5,110,903 + PS417_23630 0.45 -1.2 5,110,984 + PS417_23630 0.59 +0.5 5,111,032 + PS417_23630 0.67 -0.3 5,111,032 + PS417_23630 0.67 -1.4 5,111,033 - PS417_23630 0.67 +0.7 5,111,083 + PS417_23630 0.76 +0.5 5,111,167 + +0.4 5,111,309 + -1.4 5,111,715 + PS417_23635 0.48 -0.1 5,111,716 - PS417_23635 0.48 -0.5 5,111,716 - PS417_23635 0.48 -1.1 5,111,752 + PS417_23635 0.53 -1.2 5,111,752 + PS417_23635 0.53 +0.1 5,111,753 - PS417_23635 0.53 -1.1 5,111,804 + PS417_23635 0.60 -1.7 5,111,966 + PS417_23635 0.81 -0.1 5,111,966 + PS417_23635 0.81 -2.0 5,111,966 + PS417_23635 0.81 -0.1 5,111,966 + PS417_23635 0.81 -0.8 5,111,967 - PS417_23635 0.81 -3.2 5,111,967 - PS417_23635 0.81 -2.1 5,112,089 - -0.0 5,112,104 + +1.3 5,112,104 + -1.2 5,112,105 - -1.8 5,112,105 - -0.9 5,112,153 + +0.4 5,112,153 + -0.0 5,112,155 + -2.8 5,112,155 + +1.6 5,112,156 - -1.6 5,112,156 - -2.1 5,112,156 - -0.6 5,112,189 + +0.3 5,112,293 + +0.4 5,112,293 + -2.7 5,112,294 - -1.8 5,112,319 + -2.2 5,112,320 - +1.5 5,112,580 + PS417_23640 0.39 -0.1 5,112,795 + PS417_23640 0.63 -0.3 5,112,795 + PS417_23640 0.63 +0.0 5,112,837 + -1.0 5,112,838 - PS417_23640 0.68 -0.4 5,112,886 + PS417_23640 0.73 -1.4 5,112,886 + PS417_23640 0.73 -0.4 5,112,887 - PS417_23640 0.73 +0.8 5,112,887 - PS417_23640 0.73 +1.8 5,112,887 - PS417_23640 0.73 -1.1
Or see this region's nucleotide sequence