Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23335 and PS417_23340 are separated by 137 nucleotides PS417_23340 and PS417_23345 are separated by 12 nucleotides
PS417_23335: PS417_23335 - isoleucyl-tRNA synthetase, at 5,050,254 to 5,052,140
_23335
PS417_23340: PS417_23340 - diguanylate cyclase, at 5,052,278 to 5,053,291
_23340
PS417_23345: PS417_23345 - amino acid ABC transporter substrate-binding protein, at 5,053,304 to 5,054,332
_23345
Position (kb) 5052
5053
5054 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5051.290 kb on - strand, within PS417_23335 at 5051.326 kb on + strand, within PS417_23335 at 5051.326 kb on + strand, within PS417_23335 at 5051.446 kb on - strand, within PS417_23335 at 5051.449 kb on + strand, within PS417_23335 at 5051.449 kb on + strand, within PS417_23335 at 5051.449 kb on + strand, within PS417_23335 at 5051.449 kb on + strand at 5051.449 kb on + strand, within PS417_23335 at 5051.450 kb on - strand, within PS417_23335 at 5051.450 kb on - strand, within PS417_23335 at 5051.812 kb on + strand, within PS417_23335 at 5051.812 kb on + strand, within PS417_23335 at 5051.991 kb on - strand at 5052.134 kb on + strand at 5052.134 kb on + strand at 5052.146 kb on - strand at 5052.146 kb on - strand at 5052.201 kb on + strand at 5052.201 kb on + strand at 5052.202 kb on - strand at 5052.202 kb on - strand at 5052.232 kb on - strand at 5052.425 kb on + strand, within PS417_23340 at 5052.490 kb on - strand, within PS417_23340 at 5052.533 kb on + strand at 5052.533 kb on + strand, within PS417_23340 at 5052.534 kb on - strand, within PS417_23340 at 5052.596 kb on + strand, within PS417_23340 at 5052.596 kb on + strand, within PS417_23340 at 5052.681 kb on + strand, within PS417_23340 at 5052.681 kb on + strand, within PS417_23340 at 5052.681 kb on + strand, within PS417_23340 at 5052.681 kb on + strand, within PS417_23340 at 5052.682 kb on - strand, within PS417_23340 at 5053.251 kb on - strand at 5053.354 kb on + strand at 5053.355 kb on - strand at 5053.416 kb on + strand, within PS417_23345 at 5053.417 kb on - strand, within PS417_23345 at 5053.603 kb on + strand, within PS417_23345 at 5053.604 kb on - strand, within PS417_23345 at 5053.723 kb on - strand, within PS417_23345 at 5053.816 kb on - strand, within PS417_23345 at 5053.816 kb on - strand, within PS417_23345 at 5053.816 kb on - strand, within PS417_23345 at 5053.840 kb on - strand, within PS417_23345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,051,290 - PS417_23335 0.55 -0.6 5,051,326 + PS417_23335 0.57 -1.0 5,051,326 + PS417_23335 0.57 +0.0 5,051,446 - PS417_23335 0.63 +0.4 5,051,449 + PS417_23335 0.63 +0.6 5,051,449 + PS417_23335 0.63 +0.7 5,051,449 + PS417_23335 0.63 +0.6 5,051,449 + +0.6 5,051,449 + PS417_23335 0.63 -0.3 5,051,450 - PS417_23335 0.63 -0.5 5,051,450 - PS417_23335 0.63 -2.0 5,051,812 + PS417_23335 0.83 -2.2 5,051,812 + PS417_23335 0.83 +0.6 5,051,991 - -0.8 5,052,134 + -0.2 5,052,134 + +0.3 5,052,146 - -0.6 5,052,146 - -1.6 5,052,201 + -0.1 5,052,201 + +0.0 5,052,202 - -0.9 5,052,202 - -0.1 5,052,232 - -2.5 5,052,425 + PS417_23340 0.14 -1.6 5,052,490 - PS417_23340 0.21 -1.0 5,052,533 + +1.6 5,052,533 + PS417_23340 0.25 -0.1 5,052,534 - PS417_23340 0.25 -0.7 5,052,596 + PS417_23340 0.31 -1.4 5,052,596 + PS417_23340 0.31 +0.7 5,052,681 + PS417_23340 0.40 -1.2 5,052,681 + PS417_23340 0.40 -0.7 5,052,681 + PS417_23340 0.40 +1.6 5,052,681 + PS417_23340 0.40 -1.2 5,052,682 - PS417_23340 0.40 -2.3 5,053,251 - +0.6 5,053,354 + -0.1 5,053,355 - +0.9 5,053,416 + PS417_23345 0.11 -1.2 5,053,417 - PS417_23345 0.11 +2.4 5,053,603 + PS417_23345 0.29 +0.1 5,053,604 - PS417_23345 0.29 -0.8 5,053,723 - PS417_23345 0.41 -0.5 5,053,816 - PS417_23345 0.50 -1.9 5,053,816 - PS417_23345 0.50 -0.7 5,053,816 - PS417_23345 0.50 -0.7 5,053,840 - PS417_23345 0.52 +0.2
Or see this region's nucleotide sequence