Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23050 and PS417_23055 are separated by 238 nucleotides PS417_23055 and PS417_23060 are separated by 13 nucleotides PS417_23060 and PS417_23065 are separated by 167 nucleotides
PS417_23050: PS417_23050 - trehalose PTS system, EII-BC components TreB <small>(from data)</small>, at 4,996,268 to 4,997,710
_23050
PS417_23055: PS417_23055 - GntR family transcriptional regulator, at 4,997,949 to 4,998,653
_23055
PS417_23060: PS417_23060 - aminoglycoside phosphotransferase, at 4,998,667 to 4,999,437
_23060
PS417_23065: PS417_23065 - hypothetical protein, at 4,999,605 to 5,000,162
_23065
Position (kb) 4998
4999
5000 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4997.697 kb on + strand at 4997.698 kb on - strand at 4997.785 kb on + strand at 4997.959 kb on - strand at 4997.959 kb on - strand at 4997.971 kb on - strand at 4998.426 kb on + strand, within PS417_23055 at 4998.426 kb on + strand, within PS417_23055 at 4998.595 kb on - strand at 4998.620 kb on + strand at 4998.989 kb on + strand, within PS417_23060 at 4998.990 kb on - strand, within PS417_23060 at 4999.009 kb on - strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.065 kb on + strand, within PS417_23060 at 4999.327 kb on - strand, within PS417_23060 at 4999.327 kb on - strand, within PS417_23060 at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.375 kb on + strand at 4999.376 kb on - strand at 4999.376 kb on - strand at 4999.411 kb on + strand at 4999.412 kb on - strand at 4999.672 kb on + strand, within PS417_23065 at 4999.790 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.862 kb on + strand, within PS417_23065 at 4999.863 kb on - strand, within PS417_23065 at 4999.878 kb on - strand, within PS417_23065 at 4999.901 kb on - strand at 4999.921 kb on + strand, within PS417_23065 at 4999.921 kb on + strand, within PS417_23065 at 4999.922 kb on - strand, within PS417_23065 at 4999.922 kb on - strand, within PS417_23065 at 4999.985 kb on - strand, within PS417_23065 at 4999.985 kb on - strand, within PS417_23065 at 5000.014 kb on - strand, within PS417_23065 at 5000.014 kb on - strand, within PS417_23065 at 5000.078 kb on + strand, within PS417_23065 at 5000.117 kb on + strand at 5000.227 kb on - strand at 5000.382 kb on + strand at 5000.383 kb on - strand at 5000.385 kb on - strand at 5000.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,997,697 + +0.4 4,997,698 - +0.4 4,997,785 + +1.8 4,997,959 - +1.3 4,997,959 - -0.6 4,997,971 - +0.4 4,998,426 + PS417_23055 0.68 -2.1 4,998,426 + PS417_23055 0.68 +1.2 4,998,595 - -0.9 4,998,620 + -0.5 4,998,989 + PS417_23060 0.42 +0.9 4,998,990 - PS417_23060 0.42 +1.6 4,999,009 - PS417_23060 0.44 +1.5 4,999,065 + PS417_23060 0.52 +0.9 4,999,065 + PS417_23060 0.52 +3.5 4,999,065 + PS417_23060 0.52 -0.1 4,999,327 - PS417_23060 0.86 +0.0 4,999,327 - PS417_23060 0.86 -0.3 4,999,375 + -1.1 4,999,375 + -0.4 4,999,375 + +0.3 4,999,375 + +3.3 4,999,375 + -0.7 4,999,375 + -0.1 4,999,375 + +0.3 4,999,376 - -3.4 4,999,376 - +0.1 4,999,411 + +3.2 4,999,412 - -2.5 4,999,672 + PS417_23065 0.12 -0.4 4,999,790 + PS417_23065 0.33 +0.6 4,999,862 + PS417_23065 0.46 +0.6 4,999,862 + PS417_23065 0.46 -0.5 4,999,862 + PS417_23065 0.46 +0.8 4,999,862 + PS417_23065 0.46 +2.3 4,999,863 - PS417_23065 0.46 -1.4 4,999,878 - PS417_23065 0.49 -1.7 4,999,901 - -0.7 4,999,921 + PS417_23065 0.57 +0.3 4,999,921 + PS417_23065 0.57 -0.3 4,999,922 - PS417_23065 0.57 -0.1 4,999,922 - PS417_23065 0.57 -0.4 4,999,985 - PS417_23065 0.68 +0.3 4,999,985 - PS417_23065 0.68 +0.8 5,000,014 - PS417_23065 0.73 -0.4 5,000,014 - PS417_23065 0.73 -0.2 5,000,078 + PS417_23065 0.85 +0.8 5,000,117 + +0.4 5,000,227 - +0.1 5,000,382 + -2.2 5,000,383 - +0.5 5,000,385 - -0.3 5,000,387 - +0.4
Or see this region's nucleotide sequence