Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21895 and PS417_21900 are separated by 124 nucleotides PS417_21900 and PS417_21905 overlap by 4 nucleotides PS417_21905 and PS417_21910 are separated by 56 nucleotides
PS417_21895: PS417_21895 - nuclease PIN, at 4,751,598 to 4,753,214
_21895
PS417_21900: PS417_21900 - allophanate hydrolase, at 4,753,339 to 4,754,313
_21900
PS417_21905: PS417_21905 - allophanate hydrolase, at 4,754,310 to 4,755,188
_21905
PS417_21910: PS417_21910 - LamB/YcsF family protein, at 4,755,245 to 4,756,003
_21910
Position (kb)
4753
4754
4755 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4752.600 kb on + strand, within PS417_21895 at 4752.600 kb on + strand, within PS417_21895 at 4752.601 kb on - strand, within PS417_21895 at 4752.741 kb on + strand, within PS417_21895 at 4752.742 kb on - strand, within PS417_21895 at 4752.742 kb on - strand, within PS417_21895 at 4752.742 kb on - strand, within PS417_21895 at 4752.742 kb on - strand, within PS417_21895 at 4752.742 kb on - strand, within PS417_21895 at 4752.972 kb on + strand, within PS417_21895 at 4752.972 kb on + strand, within PS417_21895 at 4752.973 kb on - strand, within PS417_21895 at 4752.973 kb on - strand, within PS417_21895 at 4753.032 kb on + strand, within PS417_21895 at 4753.032 kb on + strand, within PS417_21895 at 4753.032 kb on + strand, within PS417_21895 at 4753.032 kb on + strand at 4753.033 kb on - strand, within PS417_21895 at 4753.033 kb on - strand, within PS417_21895 at 4753.033 kb on - strand, within PS417_21895 at 4753.122 kb on - strand at 4753.922 kb on + strand, within PS417_21900 at 4753.922 kb on + strand, within PS417_21900 at 4753.923 kb on - strand, within PS417_21900 at 4753.980 kb on - strand, within PS417_21900 at 4753.980 kb on - strand, within PS417_21900 at 4754.210 kb on + strand, within PS417_21900 at 4754.249 kb on + strand at 4754.249 kb on + strand at 4754.250 kb on - strand at 4754.250 kb on - strand at 4754.253 kb on - strand at 4754.593 kb on + strand, within PS417_21905 at 4754.659 kb on + strand, within PS417_21905 at 4754.812 kb on + strand, within PS417_21905 at 4754.891 kb on - strand, within PS417_21905 at 4754.891 kb on - strand, within PS417_21905 at 4755.036 kb on + strand, within PS417_21905 at 4755.036 kb on + strand, within PS417_21905 at 4755.036 kb on + strand, within PS417_21905 at 4755.037 kb on - strand at 4755.037 kb on - strand at 4755.037 kb on - strand, within PS417_21905 at 4755.037 kb on - strand, within PS417_21905 at 4755.212 kb on + strand at 4755.212 kb on + strand at 4755.212 kb on + strand at 4755.212 kb on + strand at 4755.213 kb on - strand at 4755.213 kb on - strand at 4755.247 kb on - strand at 4755.247 kb on - strand at 4755.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,752,600 + PS417_21895 0.62 -2.0 4,752,600 + PS417_21895 0.62 -0.9 4,752,601 - PS417_21895 0.62 +1.6 4,752,741 + PS417_21895 0.71 +0.3 4,752,742 - PS417_21895 0.71 +1.6 4,752,742 - PS417_21895 0.71 -0.6 4,752,742 - PS417_21895 0.71 -0.8 4,752,742 - PS417_21895 0.71 -0.1 4,752,742 - PS417_21895 0.71 +0.8 4,752,972 + PS417_21895 0.85 -1.5 4,752,972 + PS417_21895 0.85 -0.6 4,752,973 - PS417_21895 0.85 -1.6 4,752,973 - PS417_21895 0.85 -0.5 4,753,032 + PS417_21895 0.89 -0.7 4,753,032 + PS417_21895 0.89 +0.3 4,753,032 + PS417_21895 0.89 -0.7 4,753,032 + +1.6 4,753,033 - PS417_21895 0.89 +0.1 4,753,033 - PS417_21895 0.89 -0.2 4,753,033 - PS417_21895 0.89 +1.0 4,753,122 - +0.6 4,753,922 + PS417_21900 0.60 -1.9 4,753,922 + PS417_21900 0.60 +0.6 4,753,923 - PS417_21900 0.60 -1.7 4,753,980 - PS417_21900 0.66 -0.1 4,753,980 - PS417_21900 0.66 -0.8 4,754,210 + PS417_21900 0.89 -1.0 4,754,249 + -0.9 4,754,249 + -0.1 4,754,250 - +0.0 4,754,250 - +1.8 4,754,253 - -1.9 4,754,593 + PS417_21905 0.32 -1.0 4,754,659 + PS417_21905 0.40 -0.5 4,754,812 + PS417_21905 0.57 -0.3 4,754,891 - PS417_21905 0.66 -0.2 4,754,891 - PS417_21905 0.66 -0.9 4,755,036 + PS417_21905 0.83 +1.3 4,755,036 + PS417_21905 0.83 -0.4 4,755,036 + PS417_21905 0.83 +0.1 4,755,037 - -0.7 4,755,037 - -1.4 4,755,037 - PS417_21905 0.83 -0.3 4,755,037 - PS417_21905 0.83 +0.4 4,755,212 + -2.3 4,755,212 + +0.5 4,755,212 + +0.3 4,755,212 + +1.1 4,755,213 - +3.2 4,755,213 - -0.4 4,755,247 - -0.7 4,755,247 - -0.7 4,755,247 - +3.6
Or see this region's nucleotide sequence