Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21205 and PS417_21210 overlap by 4 nucleotides PS417_21210 and PS417_21215 are separated by 83 nucleotides PS417_21215 and PS417_21220 are separated by 16 nucleotides
PS417_21205: PS417_21205 - magnesium transporter, at 4,611,155 to 4,612,126
_21205
PS417_21210: PS417_21210 - amidotransferase, at 4,612,123 to 4,612,848
_21210
PS417_21215: PS417_21215 - 3-hydroxyacyl-CoA dehydrogenase, at 4,612,932 to 4,615,079
_21215
PS417_21220: PS417_21220 - acetyl-CoA acetyltransferase, at 4,615,096 to 4,616,301
_21220
Position (kb)
4612
4613
4614
4615
4616 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4612.001 kb on + strand, within PS417_21205 at 4612.002 kb on - strand, within PS417_21205 at 4612.002 kb on - strand, within PS417_21205 at 4612.002 kb on - strand, within PS417_21205 at 4612.044 kb on - strand at 4612.049 kb on + strand at 4612.049 kb on + strand at 4612.124 kb on + strand at 4612.125 kb on - strand at 4612.224 kb on - strand, within PS417_21210 at 4612.601 kb on + strand, within PS417_21210 at 4612.602 kb on - strand, within PS417_21210 at 4612.719 kb on - strand, within PS417_21210 at 4613.028 kb on + strand at 4613.029 kb on - strand at 4613.057 kb on + strand at 4613.216 kb on + strand, within PS417_21215 at 4613.216 kb on + strand, within PS417_21215 at 4613.217 kb on - strand, within PS417_21215 at 4613.217 kb on - strand, within PS417_21215 at 4613.263 kb on + strand, within PS417_21215 at 4613.263 kb on + strand, within PS417_21215 at 4613.263 kb on + strand at 4613.263 kb on + strand, within PS417_21215 at 4613.284 kb on + strand at 4613.285 kb on - strand, within PS417_21215 at 4613.290 kb on + strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4613.291 kb on - strand, within PS417_21215 at 4614.139 kb on + strand, within PS417_21215 at 4614.140 kb on - strand, within PS417_21215 at 4614.351 kb on - strand, within PS417_21215 at 4614.526 kb on - strand at 4614.706 kb on + strand, within PS417_21215 at 4614.915 kb on + strand at 4615.067 kb on + strand at 4615.067 kb on + strand at 4615.067 kb on + strand at 4615.583 kb on + strand, within PS417_21220 at 4615.583 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.881 kb on + strand, within PS417_21220 at 4615.881 kb on + strand at 4615.882 kb on - strand, within PS417_21220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,612,001 + PS417_21205 0.87 +0.0 4,612,002 - PS417_21205 0.87 -1.9 4,612,002 - PS417_21205 0.87 -1.0 4,612,002 - PS417_21205 0.87 -1.5 4,612,044 - -0.4 4,612,049 + +1.0 4,612,049 + +0.1 4,612,124 + +1.1 4,612,125 - +0.6 4,612,224 - PS417_21210 0.14 -1.9 4,612,601 + PS417_21210 0.66 +1.9 4,612,602 - PS417_21210 0.66 -2.2 4,612,719 - PS417_21210 0.82 +1.0 4,613,028 + -0.7 4,613,029 - -1.1 4,613,057 + -0.2 4,613,216 + PS417_21215 0.13 +1.1 4,613,216 + PS417_21215 0.13 +0.8 4,613,217 - PS417_21215 0.13 +0.4 4,613,217 - PS417_21215 0.13 +0.7 4,613,263 + PS417_21215 0.15 +1.2 4,613,263 + PS417_21215 0.15 +0.6 4,613,263 + -1.4 4,613,263 + PS417_21215 0.15 +0.7 4,613,284 + +0.0 4,613,285 - PS417_21215 0.16 -0.7 4,613,290 + PS417_21215 0.17 +1.8 4,613,291 - PS417_21215 0.17 +1.1 4,613,291 - PS417_21215 0.17 +1.0 4,613,291 - PS417_21215 0.17 +0.4 4,613,291 - PS417_21215 0.17 -1.0 4,613,291 - PS417_21215 0.17 -0.5 4,613,291 - PS417_21215 0.17 -0.7 4,613,291 - PS417_21215 0.17 -0.5 4,614,139 + PS417_21215 0.56 +0.4 4,614,140 - PS417_21215 0.56 -0.1 4,614,351 - PS417_21215 0.66 -0.0 4,614,526 - +0.2 4,614,706 + PS417_21215 0.83 +0.1 4,614,915 + +2.0 4,615,067 + -0.7 4,615,067 + -2.6 4,615,067 + -0.2 4,615,583 + PS417_21220 0.40 +0.5 4,615,583 + PS417_21220 0.40 -1.2 4,615,707 + PS417_21220 0.51 +0.9 4,615,707 + PS417_21220 0.51 +0.4 4,615,707 + PS417_21220 0.51 +0.2 4,615,881 + PS417_21220 0.65 -1.5 4,615,881 + -1.0 4,615,882 - PS417_21220 0.65 -1.1
Or see this region's nucleotide sequence