Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02085 and PS417_02090 overlap by 4 nucleotides PS417_02090 and PS417_02095 overlap by 8 nucleotides PS417_02095 and PS417_02100 overlap by 4 nucleotides PS417_02100 and PS417_02105 overlap by 4 nucleotides PS417_02105 and PS417_02110 are separated by 9 nucleotides
PS417_02085: PS417_02085 - glycosyl transferase, at 467,400 to 468,335
_02085
PS417_02090: PS417_02090 - glycosyl transferase, at 468,332 to 469,066
_02090
PS417_02095: PS417_02095 - AMP-binding protein, at 469,059 to 470,729
_02095
PS417_02100: PS417_02100 - membrane protein, at 470,726 to 471,271
_02100
PS417_02105: PS417_02105 - acyl carrier protein, at 471,268 to 471,522
_02105
PS417_02110: PS417_02110 - acyl carrier protein, at 471,532 to 471,801
_02110
Position (kb) 469
470
471 Strain fitness (log2 ratio) -2
-1
0
1
2 at 468.556 kb on - strand, within PS417_02090 at 468.678 kb on + strand, within PS417_02090 at 468.679 kb on - strand at 469.130 kb on - strand at 469.130 kb on - strand at 469.209 kb on + strand at 469.262 kb on + strand, within PS417_02095 at 469.328 kb on - strand, within PS417_02095 at 469.825 kb on + strand, within PS417_02095 at 469.826 kb on - strand, within PS417_02095 at 470.171 kb on + strand, within PS417_02095 at 470.172 kb on - strand, within PS417_02095 at 470.172 kb on - strand, within PS417_02095 at 470.219 kb on + strand, within PS417_02095 at 470.219 kb on + strand, within PS417_02095 at 470.220 kb on - strand, within PS417_02095 at 470.575 kb on + strand at 470.811 kb on + strand, within PS417_02100 at 470.811 kb on + strand, within PS417_02100 at 470.913 kb on + strand, within PS417_02100 at 470.913 kb on + strand, within PS417_02100 at 470.914 kb on - strand, within PS417_02100 at 470.983 kb on - strand, within PS417_02100 at 471.210 kb on + strand, within PS417_02100 at 471.210 kb on + strand, within PS417_02100 at 471.211 kb on - strand, within PS417_02100 at 471.211 kb on - strand, within PS417_02100 at 471.294 kb on - strand, within PS417_02105 at 471.375 kb on + strand, within PS417_02105 at 471.375 kb on + strand, within PS417_02105 at 471.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 468,556 - PS417_02090 0.30 -0.4 468,678 + PS417_02090 0.47 +0.1 468,679 - +0.6 469,130 - -0.4 469,130 - +0.9 469,209 + -0.1 469,262 + PS417_02095 0.12 -1.3 469,328 - PS417_02095 0.16 -1.4 469,825 + PS417_02095 0.46 +1.9 469,826 - PS417_02095 0.46 +1.5 470,171 + PS417_02095 0.67 -0.5 470,172 - PS417_02095 0.67 -0.6 470,172 - PS417_02095 0.67 +1.0 470,219 + PS417_02095 0.69 +0.8 470,219 + PS417_02095 0.69 +1.4 470,220 - PS417_02095 0.69 +1.2 470,575 + +0.4 470,811 + PS417_02100 0.16 -2.0 470,811 + PS417_02100 0.16 -0.1 470,913 + PS417_02100 0.34 -0.1 470,913 + PS417_02100 0.34 +1.3 470,914 - PS417_02100 0.34 +2.1 470,983 - PS417_02100 0.47 +0.5 471,210 + PS417_02100 0.89 -0.9 471,210 + PS417_02100 0.89 +0.2 471,211 - PS417_02100 0.89 +1.4 471,211 - PS417_02100 0.89 +0.1 471,294 - PS417_02105 0.10 +0.3 471,375 + PS417_02105 0.42 -0.3 471,375 + PS417_02105 0.42 -0.2 471,504 + +0.6
Or see this region's nucleotide sequence