Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02070 and PS417_02075 are separated by 29 nucleotides PS417_02075 and PS417_02080 overlap by 8 nucleotides PS417_02080 and PS417_02085 overlap by 17 nucleotides PS417_02085 and PS417_02090 overlap by 4 nucleotides
PS417_02070: PS417_02070 - membrane protein, at 464,765 to 465,427
_02070
PS417_02075: PS417_02075 - thioesterase, at 465,457 to 465,882
_02075
PS417_02080: PS417_02080 - histidine ammonia-lyase, at 465,875 to 467,416
_02080
PS417_02085: PS417_02085 - glycosyl transferase, at 467,400 to 468,335
_02085
PS417_02090: PS417_02090 - glycosyl transferase, at 468,332 to 469,066
_02090
Position (kb) 465
466
467
468 Strain fitness (log2 ratio) -2
-1
0
1
2 at 465.330 kb on - strand, within PS417_02070 at 465.476 kb on - strand at 465.596 kb on + strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 466.142 kb on + strand, within PS417_02080 at 466.142 kb on + strand, within PS417_02080 at 466.317 kb on + strand, within PS417_02080 at 466.519 kb on + strand, within PS417_02080 at 466.520 kb on - strand, within PS417_02080 at 466.945 kb on - strand, within PS417_02080 at 466.945 kb on - strand at 467.158 kb on - strand, within PS417_02080 at 467.512 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 465,330 - PS417_02070 0.85 -1.3 465,476 - +2.4 465,596 + PS417_02075 0.33 -2.1 465,597 - PS417_02075 0.33 -1.3 465,597 - PS417_02075 0.33 +0.9 465,734 + PS417_02075 0.65 -0.3 465,734 + PS417_02075 0.65 +0.7 465,734 + PS417_02075 0.65 +0.1 465,735 - PS417_02075 0.65 -0.5 465,735 - PS417_02075 0.65 +0.7 466,142 + PS417_02080 0.17 -2.1 466,142 + PS417_02080 0.17 -0.2 466,317 + PS417_02080 0.29 -0.4 466,519 + PS417_02080 0.42 +0.3 466,520 - PS417_02080 0.42 -1.3 466,945 - PS417_02080 0.69 -0.6 466,945 - -0.0 467,158 - PS417_02080 0.83 +0.0 467,512 - +0.2
Or see this region's nucleotide sequence