Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19300 and PS417_19305 are separated by 212 nucleotides PS417_19305 and PS417_19310 overlap by 4 nucleotides PS417_19310 and PS417_19315 are separated by 73 nucleotides
PS417_19300: PS417_19300 - LuxR family transcriptional regulator, at 4,186,584 to 4,186,853
_19300
PS417_19305: PS417_19305 - acireductone dioxygenase, at 4,187,066 to 4,187,596
_19305
PS417_19310: PS417_19310 - MFS transporter, at 4,187,593 to 4,188,756
_19310
PS417_19315: PS417_19315 - chorismate synthase, at 4,188,830 to 4,189,921
_19315
Position (kb)
4187
4188
4189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4186.655 kb on + strand, within PS417_19300 at 4186.698 kb on + strand, within PS417_19300 at 4186.699 kb on - strand, within PS417_19300 at 4186.699 kb on - strand, within PS417_19300 at 4186.787 kb on + strand, within PS417_19300 at 4186.788 kb on - strand, within PS417_19300 at 4186.820 kb on - strand, within PS417_19300 at 4186.839 kb on - strand at 4186.854 kb on + strand at 4186.886 kb on + strand at 4186.908 kb on - strand at 4186.920 kb on + strand at 4186.922 kb on + strand at 4186.922 kb on + strand at 4186.922 kb on + strand at 4186.923 kb on - strand at 4186.945 kb on + strand at 4186.946 kb on - strand at 4186.946 kb on - strand at 4186.946 kb on - strand at 4186.949 kb on + strand at 4186.949 kb on + strand at 4186.949 kb on + strand at 4186.949 kb on + strand at 4186.981 kb on + strand at 4186.997 kb on - strand at 4187.006 kb on + strand at 4187.007 kb on - strand at 4187.137 kb on + strand, within PS417_19305 at 4187.197 kb on + strand, within PS417_19305 at 4187.246 kb on + strand, within PS417_19305 at 4187.246 kb on + strand, within PS417_19305 at 4187.246 kb on + strand, within PS417_19305 at 4187.246 kb on + strand, within PS417_19305 at 4187.247 kb on - strand, within PS417_19305 at 4187.251 kb on - strand, within PS417_19305 at 4187.566 kb on + strand at 4187.567 kb on - strand at 4187.578 kb on - strand at 4187.589 kb on + strand at 4187.590 kb on - strand at 4187.709 kb on + strand at 4187.710 kb on - strand, within PS417_19310 at 4187.720 kb on + strand, within PS417_19310 at 4187.763 kb on - strand, within PS417_19310 at 4187.920 kb on + strand, within PS417_19310 at 4188.092 kb on + strand, within PS417_19310 at 4188.093 kb on - strand, within PS417_19310 at 4188.093 kb on - strand, within PS417_19310 at 4188.096 kb on - strand, within PS417_19310 at 4188.269 kb on - strand, within PS417_19310 at 4188.278 kb on + strand, within PS417_19310 at 4188.279 kb on - strand, within PS417_19310 at 4188.476 kb on + strand, within PS417_19310 at 4188.477 kb on - strand, within PS417_19310 at 4188.477 kb on - strand, within PS417_19310 at 4188.665 kb on + strand at 4188.716 kb on + strand at 4188.716 kb on + strand at 4188.717 kb on - strand at 4188.853 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,186,655 + PS417_19300 0.26 -0.8 4,186,698 + PS417_19300 0.42 -0.3 4,186,699 - PS417_19300 0.43 -0.6 4,186,699 - PS417_19300 0.43 -0.1 4,186,787 + PS417_19300 0.75 +0.6 4,186,788 - PS417_19300 0.76 +1.6 4,186,820 - PS417_19300 0.87 -0.5 4,186,839 - +0.1 4,186,854 + +0.2 4,186,886 + -0.4 4,186,908 - -1.0 4,186,920 + -0.3 4,186,922 + -1.2 4,186,922 + -1.2 4,186,922 + +0.8 4,186,923 - +0.6 4,186,945 + +2.3 4,186,946 - +0.6 4,186,946 - -0.1 4,186,946 - -0.9 4,186,949 + +0.4 4,186,949 + +0.6 4,186,949 + +1.2 4,186,949 + -2.4 4,186,981 + -0.4 4,186,997 - -1.8 4,187,006 + +0.7 4,187,007 - +1.1 4,187,137 + PS417_19305 0.13 -0.7 4,187,197 + PS417_19305 0.25 -2.1 4,187,246 + PS417_19305 0.34 +0.0 4,187,246 + PS417_19305 0.34 +0.2 4,187,246 + PS417_19305 0.34 +0.2 4,187,246 + PS417_19305 0.34 -0.8 4,187,247 - PS417_19305 0.34 -1.2 4,187,251 - PS417_19305 0.35 +2.0 4,187,566 + -0.7 4,187,567 - -0.3 4,187,578 - +2.0 4,187,589 + -1.6 4,187,590 - +1.0 4,187,709 + +0.5 4,187,710 - PS417_19310 0.10 -0.0 4,187,720 + PS417_19310 0.11 +0.6 4,187,763 - PS417_19310 0.15 +0.8 4,187,920 + PS417_19310 0.28 +0.6 4,188,092 + PS417_19310 0.43 +0.3 4,188,093 - PS417_19310 0.43 -0.8 4,188,093 - PS417_19310 0.43 +0.4 4,188,096 - PS417_19310 0.43 -1.0 4,188,269 - PS417_19310 0.58 +0.1 4,188,278 + PS417_19310 0.59 +1.0 4,188,279 - PS417_19310 0.59 +0.3 4,188,476 + PS417_19310 0.76 +0.4 4,188,477 - PS417_19310 0.76 +2.3 4,188,477 - PS417_19310 0.76 -0.2 4,188,665 + -1.3 4,188,716 + +0.6 4,188,716 + -1.0 4,188,717 - -0.3 4,188,853 + -1.6
Or see this region's nucleotide sequence