Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18780 and PS417_18790 are separated by 180 nucleotides PS417_18790 and PS417_18795 are separated by 112 nucleotides PS417_18795 and PS417_18800 are separated by 53 nucleotides PS417_18800 and PS417_18805 are separated by 69 nucleotides
PS417_18780: PS417_18780 - flavin reductase, at 4,073,128 to 4,073,637
_18780
PS417_18790: PS417_18790 - amino acid transporter LysE, at 4,073,818 to 4,074,426
_18790
PS417_18795: PS417_18795 - LysR family transcriptional regulator, at 4,074,539 to 4,075,402
_18795
PS417_18800: PS417_18800 - hypothetical protein, at 4,075,456 to 4,075,818
_18800
PS417_18805: PS417_18805 - hypothetical protein, at 4,075,888 to 4,077,135
_18805
Position (kb)
4074
4075
4076 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4073.749 kb on - strand at 4073.749 kb on - strand at 4073.874 kb on + strand at 4073.874 kb on + strand at 4073.875 kb on - strand at 4073.940 kb on - strand, within PS417_18790 at 4073.940 kb on - strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790 at 4074.301 kb on - strand, within PS417_18790 at 4074.401 kb on + strand at 4074.401 kb on + strand at 4074.402 kb on - strand at 4074.402 kb on - strand at 4074.402 kb on - strand at 4074.526 kb on - strand at 4074.848 kb on + strand, within PS417_18795 at 4074.849 kb on - strand, within PS417_18795 at 4074.849 kb on - strand, within PS417_18795 at 4074.849 kb on - strand, within PS417_18795 at 4074.849 kb on - strand, within PS417_18795 at 4074.849 kb on - strand, within PS417_18795 at 4075.170 kb on - strand, within PS417_18795 at 4075.170 kb on - strand, within PS417_18795 at 4075.235 kb on - strand, within PS417_18795 at 4075.321 kb on - strand at 4075.372 kb on + strand at 4075.422 kb on - strand at 4075.721 kb on + strand, within PS417_18800 at 4075.722 kb on - strand, within PS417_18800 at 4076.050 kb on + strand, within PS417_18805 at 4076.051 kb on - strand, within PS417_18805 at 4076.171 kb on - strand, within PS417_18805 at 4076.209 kb on + strand, within PS417_18805 at 4076.212 kb on + strand, within PS417_18805 at 4076.212 kb on + strand, within PS417_18805 at 4076.212 kb on + strand, within PS417_18805 at 4076.213 kb on - strand, within PS417_18805 at 4076.315 kb on - strand, within PS417_18805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,073,749 - -1.1 4,073,749 - +0.7 4,073,874 + -1.3 4,073,874 + -0.1 4,073,875 - +0.6 4,073,940 - PS417_18790 0.20 -1.2 4,073,940 - PS417_18790 0.20 +0.6 4,073,966 + PS417_18790 0.24 -0.3 4,073,966 + PS417_18790 0.24 +0.3 4,073,966 + PS417_18790 0.24 -0.5 4,073,967 - PS417_18790 0.24 -1.5 4,073,967 - PS417_18790 0.24 -1.4 4,073,967 - PS417_18790 0.24 +0.4 4,074,301 - PS417_18790 0.79 +1.7 4,074,401 + +1.7 4,074,401 + -0.3 4,074,402 - +0.8 4,074,402 - -0.0 4,074,402 - +1.3 4,074,526 - -1.6 4,074,848 + PS417_18795 0.36 -2.4 4,074,849 - PS417_18795 0.36 +2.1 4,074,849 - PS417_18795 0.36 -0.8 4,074,849 - PS417_18795 0.36 -0.7 4,074,849 - PS417_18795 0.36 -0.3 4,074,849 - PS417_18795 0.36 -0.9 4,075,170 - PS417_18795 0.73 -0.4 4,075,170 - PS417_18795 0.73 -0.3 4,075,235 - PS417_18795 0.81 -1.8 4,075,321 - -0.2 4,075,372 + +0.5 4,075,422 - -0.2 4,075,721 + PS417_18800 0.73 +0.4 4,075,722 - PS417_18800 0.73 +1.0 4,076,050 + PS417_18805 0.13 -0.5 4,076,051 - PS417_18805 0.13 -0.3 4,076,171 - PS417_18805 0.23 -0.9 4,076,209 + PS417_18805 0.26 -2.3 4,076,212 + PS417_18805 0.26 +0.6 4,076,212 + PS417_18805 0.26 -0.2 4,076,212 + PS417_18805 0.26 +0.2 4,076,213 - PS417_18805 0.26 -0.3 4,076,315 - PS417_18805 0.34 +1.5
Or see this region's nucleotide sequence