Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18470 and PS417_18475 are separated by 15 nucleotides PS417_18475 and PS417_18480 are separated by 7 nucleotides PS417_18480 and PS417_18485 overlap by 4 nucleotides PS417_18485 and PS417_18490 are separated by 215 nucleotides
PS417_18470: PS417_18470 - hypothetical protein, at 4,016,402 to 4,016,626
_18470
PS417_18475: PS417_18475 - Pyrrolidone-carboxylate peptidase, at 4,016,642 to 4,017,283
_18475
PS417_18480: PS417_18480 - permease, at 4,017,291 to 4,018,241
_18480
PS417_18485: PS417_18485 - membrane protein, at 4,018,238 to 4,018,954
_18485
PS417_18490: PS417_18490 - hypothetical protein, at 4,019,170 to 4,019,418
_18490
Position (kb)
4017
4018
4019 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4016.295 kb on + strand at 4016.317 kb on + strand at 4016.409 kb on + strand at 4016.409 kb on + strand at 4016.416 kb on - strand at 4016.416 kb on - strand at 4016.549 kb on + strand, within PS417_18470 at 4016.549 kb on + strand, within PS417_18470 at 4016.550 kb on - strand, within PS417_18470 at 4016.581 kb on - strand, within PS417_18470 at 4016.683 kb on + strand at 4016.683 kb on + strand at 4016.684 kb on - strand at 4016.728 kb on + strand, within PS417_18475 at 4016.729 kb on - strand, within PS417_18475 at 4016.729 kb on - strand, within PS417_18475 at 4016.729 kb on - strand, within PS417_18475 at 4017.272 kb on + strand at 4017.312 kb on + strand at 4017.312 kb on + strand at 4017.313 kb on - strand at 4017.317 kb on + strand at 4017.496 kb on + strand, within PS417_18480 at 4017.594 kb on + strand, within PS417_18480 at 4017.595 kb on - strand, within PS417_18480 at 4017.598 kb on + strand, within PS417_18480 at 4017.598 kb on + strand, within PS417_18480 at 4017.599 kb on - strand, within PS417_18480 at 4017.599 kb on - strand, within PS417_18480 at 4017.599 kb on - strand, within PS417_18480 at 4017.599 kb on - strand at 4017.599 kb on - strand at 4017.599 kb on - strand, within PS417_18480 at 4017.635 kb on + strand, within PS417_18480 at 4017.637 kb on + strand, within PS417_18480 at 4017.637 kb on + strand, within PS417_18480 at 4017.637 kb on + strand, within PS417_18480 at 4017.637 kb on + strand, within PS417_18480 at 4017.638 kb on - strand, within PS417_18480 at 4017.638 kb on - strand at 4017.638 kb on - strand, within PS417_18480 at 4017.638 kb on - strand, within PS417_18480 at 4017.638 kb on - strand, within PS417_18480 at 4017.690 kb on + strand, within PS417_18480 at 4017.956 kb on + strand, within PS417_18480 at 4017.956 kb on + strand, within PS417_18480 at 4017.956 kb on + strand, within PS417_18480 at 4017.957 kb on - strand, within PS417_18480 at 4017.957 kb on - strand, within PS417_18480 at 4017.957 kb on - strand, within PS417_18480 at 4018.194 kb on + strand at 4018.217 kb on - strand at 4018.222 kb on + strand at 4018.223 kb on - strand at 4018.235 kb on + strand at 4018.644 kb on + strand, within PS417_18485 at 4018.644 kb on + strand, within PS417_18485 at 4018.644 kb on + strand, within PS417_18485 at 4018.645 kb on - strand, within PS417_18485 at 4018.645 kb on - strand, within PS417_18485 at 4018.645 kb on - strand, within PS417_18485 at 4018.916 kb on + strand at 4018.968 kb on + strand at 4018.969 kb on - strand at 4019.051 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,016,295 + +1.1 4,016,317 + +0.9 4,016,409 + +0.5 4,016,409 + -1.3 4,016,416 - +1.3 4,016,416 - -0.5 4,016,549 + PS417_18470 0.65 -1.9 4,016,549 + PS417_18470 0.65 -1.3 4,016,550 - PS417_18470 0.66 -1.5 4,016,581 - PS417_18470 0.80 +1.0 4,016,683 + +1.4 4,016,683 + -0.4 4,016,684 - -0.7 4,016,728 + PS417_18475 0.13 +0.2 4,016,729 - PS417_18475 0.14 +0.6 4,016,729 - PS417_18475 0.14 -0.2 4,016,729 - PS417_18475 0.14 +0.0 4,017,272 + -0.5 4,017,312 + -1.5 4,017,312 + +0.6 4,017,313 - -1.3 4,017,317 + -0.7 4,017,496 + PS417_18480 0.22 -0.4 4,017,594 + PS417_18480 0.32 +0.9 4,017,595 - PS417_18480 0.32 +0.2 4,017,598 + PS417_18480 0.32 -1.2 4,017,598 + PS417_18480 0.32 -1.0 4,017,599 - PS417_18480 0.32 +0.7 4,017,599 - PS417_18480 0.32 +0.8 4,017,599 - PS417_18480 0.32 +1.7 4,017,599 - +0.1 4,017,599 - +1.7 4,017,599 - PS417_18480 0.32 +1.3 4,017,635 + PS417_18480 0.36 +1.6 4,017,637 + PS417_18480 0.36 +0.0 4,017,637 + PS417_18480 0.36 +1.1 4,017,637 + PS417_18480 0.36 +1.1 4,017,637 + PS417_18480 0.36 +0.9 4,017,638 - PS417_18480 0.36 -0.0 4,017,638 - +1.7 4,017,638 - PS417_18480 0.36 -1.0 4,017,638 - PS417_18480 0.36 -0.1 4,017,638 - PS417_18480 0.36 +0.0 4,017,690 + PS417_18480 0.42 -0.1 4,017,956 + PS417_18480 0.70 -0.4 4,017,956 + PS417_18480 0.70 +0.2 4,017,956 + PS417_18480 0.70 -0.9 4,017,957 - PS417_18480 0.70 +1.4 4,017,957 - PS417_18480 0.70 -2.8 4,017,957 - PS417_18480 0.70 +0.3 4,018,194 + +0.1 4,018,217 - +1.3 4,018,222 + -0.3 4,018,223 - -0.7 4,018,235 + -0.9 4,018,644 + PS417_18485 0.57 +0.1 4,018,644 + PS417_18485 0.57 -1.0 4,018,644 + PS417_18485 0.57 +0.2 4,018,645 - PS417_18485 0.57 +0.3 4,018,645 - PS417_18485 0.57 +0.8 4,018,645 - PS417_18485 0.57 +0.1 4,018,916 + +1.0 4,018,968 + -1.0 4,018,969 - +0.3 4,019,051 + -1.4
Or see this region's nucleotide sequence