Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18230 and PS417_18235 are separated by 73 nucleotides PS417_18235 and PS417_18240 are separated by 41 nucleotides PS417_18240 and PS417_18245 overlap by 4 nucleotides
PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686
_18230
PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353
_18235
PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057
_18240
PS417_18245: PS417_18245 - hypothetical protein, at 3,975,054 to 3,976,313
_18245
Position (kb)
3973
3974
3975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3972.788 kb on - strand at 3972.801 kb on + strand, within PS417_18230 at 3972.801 kb on + strand, within PS417_18230 at 3972.820 kb on + strand, within PS417_18230 at 3972.820 kb on + strand, within PS417_18230 at 3972.964 kb on + strand, within PS417_18230 at 3972.964 kb on + strand, within PS417_18230 at 3972.965 kb on - strand, within PS417_18230 at 3972.965 kb on - strand, within PS417_18230 at 3973.228 kb on + strand, within PS417_18230 at 3973.229 kb on - strand, within PS417_18230 at 3973.336 kb on + strand, within PS417_18230 at 3973.393 kb on + strand, within PS417_18230 at 3973.489 kb on + strand, within PS417_18230 at 3973.490 kb on - strand, within PS417_18230 at 3973.618 kb on + strand at 3973.733 kb on - strand at 3973.932 kb on + strand, within PS417_18235 at 3974.016 kb on - strand, within PS417_18235 at 3974.241 kb on - strand, within PS417_18235 at 3974.457 kb on + strand at 3974.477 kb on + strand, within PS417_18240 at 3974.477 kb on + strand, within PS417_18240 at 3974.507 kb on - strand, within PS417_18240 at 3974.615 kb on - strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240 at 3974.800 kb on + strand, within PS417_18240 at 3974.801 kb on - strand, within PS417_18240 at 3974.801 kb on - strand, within PS417_18240 at 3974.830 kb on + strand, within PS417_18240 at 3974.831 kb on - strand, within PS417_18240 at 3974.831 kb on - strand, within PS417_18240 at 3974.935 kb on + strand, within PS417_18240 at 3974.935 kb on + strand, within PS417_18240 at 3974.936 kb on - strand, within PS417_18240 at 3974.954 kb on + strand, within PS417_18240 at 3975.016 kb on + strand at 3975.183 kb on - strand, within PS417_18245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,972,788 - -1.1 3,972,801 + PS417_18230 0.42 -0.5 3,972,801 + PS417_18230 0.42 +0.8 3,972,820 + PS417_18230 0.43 -0.5 3,972,820 + PS417_18230 0.43 +0.1 3,972,964 + PS417_18230 0.52 -0.4 3,972,964 + PS417_18230 0.52 -1.8 3,972,965 - PS417_18230 0.52 +0.5 3,972,965 - PS417_18230 0.52 -0.6 3,973,228 + PS417_18230 0.70 +1.5 3,973,229 - PS417_18230 0.70 -0.2 3,973,336 + PS417_18230 0.77 -0.7 3,973,393 + PS417_18230 0.81 -1.8 3,973,489 + PS417_18230 0.87 -1.3 3,973,490 - PS417_18230 0.87 +0.8 3,973,618 + +1.3 3,973,733 - -0.4 3,973,932 + PS417_18235 0.29 +2.0 3,974,016 - PS417_18235 0.43 +2.2 3,974,241 - PS417_18235 0.81 -0.1 3,974,457 + -0.9 3,974,477 + PS417_18240 0.12 -1.3 3,974,477 + PS417_18240 0.12 -2.2 3,974,507 - PS417_18240 0.17 -0.5 3,974,615 - PS417_18240 0.33 -3.4 3,974,623 + PS417_18240 0.34 -0.1 3,974,623 + PS417_18240 0.34 -0.4 3,974,624 - PS417_18240 0.35 +0.2 3,974,624 - PS417_18240 0.35 -0.2 3,974,800 + PS417_18240 0.61 +0.4 3,974,801 - PS417_18240 0.61 -1.4 3,974,801 - PS417_18240 0.61 +0.0 3,974,830 + PS417_18240 0.66 -0.3 3,974,831 - PS417_18240 0.66 -2.1 3,974,831 - PS417_18240 0.66 -1.8 3,974,935 + PS417_18240 0.81 +1.9 3,974,935 + PS417_18240 0.81 +0.7 3,974,936 - PS417_18240 0.82 +0.3 3,974,954 + PS417_18240 0.84 -0.0 3,975,016 + -0.8 3,975,183 - PS417_18245 0.10 -0.6
Or see this region's nucleotide sequence