Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18110 and PS417_18115 are separated by 84 nucleotides PS417_18115 and PS417_18120 are separated by 36 nucleotides PS417_18120 and PS417_18125 are separated by 2 nucleotides
PS417_18110: PS417_18110 - TonB-dependent receptor, at 3,944,037 to 3,946,427
_18110
PS417_18115: PS417_18115 - gamma-glutamyltransferase, at 3,946,512 to 3,948,122
_18115
PS417_18120: PS417_18120 - arginine ABC transporter ATP-binding protein, at 3,948,159 to 3,948,938
_18120
PS417_18125: PS417_18125 - polar amino acid ABC transporter permease, at 3,948,941 to 3,949,717
_18125
Position (kb)
3946
3947
3948
3949 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3945.782 kb on - strand, within PS417_18110 at 3945.793 kb on + strand, within PS417_18110 at 3945.943 kb on + strand, within PS417_18110 at 3946.118 kb on + strand at 3946.118 kb on + strand, within PS417_18110 at 3946.120 kb on + strand, within PS417_18110 at 3946.120 kb on + strand, within PS417_18110 at 3946.195 kb on + strand at 3946.196 kb on - strand at 3946.319 kb on - strand at 3946.319 kb on - strand at 3946.319 kb on - strand at 3946.349 kb on - strand at 3946.444 kb on + strand at 3946.444 kb on + strand at 3946.444 kb on + strand at 3946.445 kb on - strand at 3946.513 kb on + strand at 3946.563 kb on + strand at 3946.563 kb on + strand at 3946.563 kb on + strand at 3946.564 kb on - strand at 3947.053 kb on + strand, within PS417_18115 at 3947.191 kb on + strand, within PS417_18115 at 3947.192 kb on - strand at 3947.420 kb on - strand, within PS417_18115 at 3947.633 kb on + strand, within PS417_18115 at 3947.795 kb on - strand, within PS417_18115 at 3947.795 kb on - strand, within PS417_18115 at 3948.127 kb on - strand at 3948.173 kb on + strand at 3948.174 kb on - strand at 3948.890 kb on + strand at 3948.891 kb on - strand at 3949.087 kb on - strand, within PS417_18125 at 3949.104 kb on + strand, within PS417_18125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,945,782 - PS417_18110 0.73 +1.2 3,945,793 + PS417_18110 0.73 +0.2 3,945,943 + PS417_18110 0.80 -1.0 3,946,118 + +1.7 3,946,118 + PS417_18110 0.87 -0.5 3,946,120 + PS417_18110 0.87 -0.8 3,946,120 + PS417_18110 0.87 -0.4 3,946,195 + +0.3 3,946,196 - -2.3 3,946,319 - +0.4 3,946,319 - +0.6 3,946,319 - -1.0 3,946,349 - +1.8 3,946,444 + -1.6 3,946,444 + -0.4 3,946,444 + +0.2 3,946,445 - +2.7 3,946,513 + -1.7 3,946,563 + -0.1 3,946,563 + +0.7 3,946,563 + +0.2 3,946,564 - +0.7 3,947,053 + PS417_18115 0.34 +0.3 3,947,191 + PS417_18115 0.42 -0.4 3,947,192 - +0.7 3,947,420 - PS417_18115 0.56 -0.1 3,947,633 + PS417_18115 0.70 -2.6 3,947,795 - PS417_18115 0.80 -0.2 3,947,795 - PS417_18115 0.80 -1.1 3,948,127 - -0.1 3,948,173 + -0.9 3,948,174 - -0.7 3,948,890 + -1.6 3,948,891 - -0.1 3,949,087 - PS417_18125 0.19 -0.8 3,949,104 + PS417_18125 0.21 -0.5
Or see this region's nucleotide sequence