Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17510 and PS417_17515 overlap by 20 nucleotides PS417_17515 and PS417_17520 are separated by 135 nucleotides PS417_17520 and PS417_17525 are separated by 0 nucleotides
PS417_17510: PS417_17510 - histidine kinase, at 3,824,007 to 3,825,908
_17510
PS417_17515: PS417_17515 - GfdT protein, at 3,825,889 to 3,827,034
_17515
PS417_17520: PS417_17520 - membrane protein, at 3,827,170 to 3,827,976
_17520
PS417_17525: PS417_17525 - pyridine nucleotide-disulfide oxidoreductase, at 3,827,977 to 3,829,221
_17525
Position (kb)
3825
3826
3827
3828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3825.165 kb on + strand, within PS417_17510 at 3825.166 kb on - strand, within PS417_17510 at 3825.166 kb on - strand, within PS417_17510 at 3825.320 kb on + strand, within PS417_17510 at 3825.331 kb on + strand, within PS417_17510 at 3825.332 kb on - strand, within PS417_17510 at 3825.422 kb on - strand, within PS417_17510 at 3825.502 kb on + strand, within PS417_17510 at 3825.535 kb on + strand at 3825.535 kb on + strand, within PS417_17510 at 3825.778 kb on + strand at 3825.936 kb on + strand at 3825.937 kb on - strand at 3825.937 kb on - strand at 3826.232 kb on + strand, within PS417_17515 at 3826.232 kb on + strand, within PS417_17515 at 3826.233 kb on - strand, within PS417_17515 at 3826.233 kb on - strand, within PS417_17515 at 3826.233 kb on - strand, within PS417_17515 at 3826.236 kb on - strand, within PS417_17515 at 3826.236 kb on - strand, within PS417_17515 at 3826.502 kb on + strand, within PS417_17515 at 3827.015 kb on - strand at 3827.015 kb on - strand at 3827.063 kb on + strand at 3827.086 kb on + strand at 3827.087 kb on - strand at 3827.091 kb on + strand at 3827.091 kb on + strand at 3827.091 kb on + strand at 3827.092 kb on - strand at 3827.092 kb on - strand at 3827.092 kb on - strand at 3827.092 kb on - strand at 3827.092 kb on - strand at 3827.092 kb on - strand at 3827.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,825,165 + PS417_17510 0.61 -1.9 3,825,166 - PS417_17510 0.61 +0.1 3,825,166 - PS417_17510 0.61 +0.3 3,825,320 + PS417_17510 0.69 -1.8 3,825,331 + PS417_17510 0.70 +1.0 3,825,332 - PS417_17510 0.70 -0.5 3,825,422 - PS417_17510 0.74 -1.2 3,825,502 + PS417_17510 0.79 -0.3 3,825,535 + -1.3 3,825,535 + PS417_17510 0.80 -1.2 3,825,778 + +0.5 3,825,936 + -0.6 3,825,937 - +0.2 3,825,937 - -0.3 3,826,232 + PS417_17515 0.30 -0.8 3,826,232 + PS417_17515 0.30 -1.8 3,826,233 - PS417_17515 0.30 -1.3 3,826,233 - PS417_17515 0.30 +0.2 3,826,233 - PS417_17515 0.30 -2.4 3,826,236 - PS417_17515 0.30 -1.0 3,826,236 - PS417_17515 0.30 -2.9 3,826,502 + PS417_17515 0.53 +0.1 3,827,015 - -1.0 3,827,015 - -1.3 3,827,063 + +0.2 3,827,086 + +0.0 3,827,087 - +0.5 3,827,091 + -0.4 3,827,091 + -0.6 3,827,091 + -0.5 3,827,092 - -0.7 3,827,092 - +0.3 3,827,092 - -0.1 3,827,092 - -1.4 3,827,092 - +5.9 3,827,092 - -1.5 3,827,950 + +0.2
Or see this region's nucleotide sequence