Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16045 and PS417_16050 are separated by 205 nucleotides PS417_16050 and PS417_16055 overlap by 4 nucleotides PS417_16055 and PS417_16060 are separated by 15 nucleotides PS417_16060 and PS417_16065 are separated by 46 nucleotides
PS417_16045: PS417_16045 - acyl-CoA dehydrogenase, at 3,516,279 to 3,517,457
_16045
PS417_16050: PS417_16050 - energy transducer TonB, at 3,517,663 to 3,518,436
_16050
PS417_16055: PS417_16055 - biopolymer transporter TolR, at 3,518,433 to 3,518,879
_16055
PS417_16060: PS417_16060 - protein tolQ, at 3,518,895 to 3,519,587
_16060
PS417_16065: PS417_16065 - 3-phytase, at 3,519,634 to 3,521,499
_16065
Position (kb)
3518
3519 Strain fitness (log2 ratio)
-1
0
1
2 at 3517.452 kb on - strand at 3517.452 kb on - strand at 3517.526 kb on + strand at 3517.559 kb on - strand at 3517.664 kb on + strand at 3517.664 kb on + strand at 3518.243 kb on - strand, within PS417_16050 at 3518.256 kb on + strand, within PS417_16050 at 3518.470 kb on - strand at 3518.542 kb on + strand, within PS417_16055 at 3518.542 kb on + strand, within PS417_16055 at 3518.543 kb on - strand, within PS417_16055 at 3518.975 kb on - strand, within PS417_16060 at 3519.178 kb on + strand, within PS417_16060 at 3519.178 kb on + strand at 3519.179 kb on - strand, within PS417_16060 at 3519.313 kb on + strand, within PS417_16060 at 3519.566 kb on + strand at 3519.566 kb on + strand at 3519.567 kb on - strand at 3519.567 kb on - strand at 3519.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,517,452 - +1.3 3,517,452 - +0.5 3,517,526 + +1.7 3,517,559 - -0.0 3,517,664 + -0.1 3,517,664 + +0.5 3,518,243 - PS417_16050 0.75 +0.2 3,518,256 + PS417_16050 0.77 +1.8 3,518,470 - +0.8 3,518,542 + PS417_16055 0.24 -0.2 3,518,542 + PS417_16055 0.24 -1.0 3,518,543 - PS417_16055 0.25 -0.1 3,518,975 - PS417_16060 0.12 +1.0 3,519,178 + PS417_16060 0.41 -0.5 3,519,178 + -0.8 3,519,179 - PS417_16060 0.41 -0.7 3,519,313 + PS417_16060 0.60 -1.1 3,519,566 + -1.7 3,519,566 + +1.8 3,519,567 - -0.1 3,519,567 - -0.6 3,519,636 - -1.2
Or see this region's nucleotide sequence