Strain Fitness in Pseudomonas simiae WCS417 around PS417_01555

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01550 and PS417_01555 are separated by 325 nucleotidesPS417_01555 and PS417_01560 are separated by 231 nucleotidesPS417_01560 and PS417_01565 overlap by 1 nucleotides PS417_01550: PS417_01550 - cell envelope biogenesis protein AsmA, at 341,729 to 343,939 _01550 PS417_01555: PS417_01555 - MFS transporter, at 344,265 to 345,926 _01555 PS417_01560: PS417_01560 - imidazoleglycerol-phosphate dehydratase, at 346,158 to 346,751 _01560 PS417_01565: PS417_01565 - imidazole glycerol phosphate synthase, at 346,751 to 347,389 _01565 Position (kb) 344 345 346Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 343.411 kb on - strand, within PS417_01550at 343.608 kb on - strand, within PS417_01550at 343.613 kb on + strand, within PS417_01550at 343.834 kb on + strandat 343.834 kb on + strandat 343.835 kb on - strandat 343.835 kb on - strandat 343.835 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.874 kb on + strandat 343.875 kb on - strandat 343.907 kb on + strandat 343.908 kb on - strandat 343.953 kb on + strandat 343.954 kb on - strandat 343.954 kb on - strandat 343.982 kb on + strandat 343.982 kb on + strandat 343.983 kb on - strandat 343.983 kb on - strandat 343.983 kb on - strandat 343.984 kb on + strandat 344.020 kb on - strandat 344.027 kb on - strandat 344.089 kb on - strandat 344.122 kb on - strandat 344.122 kb on - strandat 344.167 kb on - strandat 344.242 kb on - strandat 344.242 kb on - strandat 344.242 kb on - strandat 344.245 kb on - strandat 344.406 kb on + strandat 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.622 kb on - strand, within PS417_01555at 344.682 kb on - strand, within PS417_01555at 344.685 kb on + strandat 344.685 kb on + strand, within PS417_01555at 344.685 kb on + strand, within PS417_01555at 344.685 kb on + strand, within PS417_01555at 344.686 kb on - strand, within PS417_01555at 344.736 kb on - strand, within PS417_01555at 344.754 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.757 kb on - strand, within PS417_01555at 345.351 kb on - strand, within PS417_01555at 345.351 kb on - strand, within PS417_01555at 345.355 kb on - strand, within PS417_01555at 345.355 kb on - strand, within PS417_01555at 345.465 kb on + strand, within PS417_01555at 345.466 kb on - strand, within PS417_01555at 345.597 kb on + strand, within PS417_01555at 345.597 kb on + strand, within PS417_01555at 345.598 kb on - strand, within PS417_01555at 345.598 kb on - strand, within PS417_01555at 345.661 kb on - strand, within PS417_01555at 345.661 kb on - strand, within PS417_01555at 345.729 kb on + strand, within PS417_01555at 345.914 kb on - strandat 345.955 kb on - strandat 345.986 kb on + strandat 346.042 kb on + strandat 346.042 kb on + strandat 346.042 kb on + strandat 346.042 kb on + strandat 346.042 kb on + strandat 346.042 kb on + strandat 346.043 kb on - strandat 346.043 kb on - strandat 346.043 kb on - strandat 346.115 kb on - strandat 346.116 kb on + strandat 346.116 kb on + strandat 346.169 kb on + strandat 346.170 kb on - strandat 346.170 kb on - strandat 346.406 kb on + strand, within PS417_01560at 346.407 kb on - strand, within PS417_01560at 346.446 kb on + strand, within PS417_01560at 346.446 kb on + strand, within PS417_01560at 346.524 kb on + strand, within PS417_01560at 346.822 kb on - strand, within PS417_01565

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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343,411 - PS417_01550 0.76 +1.2
343,608 - PS417_01550 0.85 +1.0
343,613 + PS417_01550 0.85 +0.7
343,834 + +1.8
343,834 + +0.1
343,835 - +0.3
343,835 - +1.5
343,835 - +1.0
343,837 - +0.8
343,837 - +1.5
343,837 - +0.0
343,837 - +2.6
343,874 + +1.1
343,875 - +1.4
343,907 + +0.6
343,908 - +1.4
343,953 + +0.5
343,954 - -0.5
343,954 - -0.9
343,982 + -0.1
343,982 + +0.1
343,983 - -1.9
343,983 - -0.7
343,983 - -0.6
343,984 + -2.2
344,020 - -0.8
344,027 - +0.5
344,089 - -1.7
344,122 - -2.1
344,122 - -2.4
344,167 - -0.1
344,242 - -0.6
344,242 - -1.3
344,242 - -2.7
344,245 - -0.0
344,406 + -0.9
344,547 + PS417_01555 0.17 +0.6
344,547 + PS417_01555 0.17 -0.4
344,547 + PS417_01555 0.17 +2.9
344,547 + PS417_01555 0.17 +0.1
344,548 - PS417_01555 0.17 +0.8
344,548 - PS417_01555 0.17 -1.1
344,548 - PS417_01555 0.17 +0.1
344,548 - PS417_01555 0.17 -0.8
344,548 - PS417_01555 0.17 -1.0
344,548 - PS417_01555 0.17 +1.0
344,621 + PS417_01555 0.21 -0.8
344,621 + PS417_01555 0.21 +0.7
344,621 + PS417_01555 0.21 -1.8
344,621 + PS417_01555 0.21 +0.5
344,622 - PS417_01555 0.21 +1.2
344,682 - PS417_01555 0.25 -0.9
344,685 + +1.5
344,685 + PS417_01555 0.25 -0.3
344,685 + PS417_01555 0.25 +4.2
344,685 + PS417_01555 0.25 -1.6
344,686 - PS417_01555 0.25 -0.7
344,736 - PS417_01555 0.28 +2.4
344,754 + PS417_01555 0.29 -0.5
344,756 + PS417_01555 0.30 -0.5
344,756 + PS417_01555 0.30 +0.7
344,756 + PS417_01555 0.30 -0.0
344,756 + PS417_01555 0.30 +1.2
344,757 - PS417_01555 0.30 +1.8
345,351 - PS417_01555 0.65 +0.7
345,351 - PS417_01555 0.65 -0.3
345,355 - PS417_01555 0.66 -0.2
345,355 - PS417_01555 0.66 +0.5
345,465 + PS417_01555 0.72 -0.8
345,466 - PS417_01555 0.72 -0.9
345,597 + PS417_01555 0.80 -0.5
345,597 + PS417_01555 0.80 -0.6
345,598 - PS417_01555 0.80 -0.4
345,598 - PS417_01555 0.80 -0.8
345,661 - PS417_01555 0.84 +0.4
345,661 - PS417_01555 0.84 +1.2
345,729 + PS417_01555 0.88 +0.5
345,914 - -1.7
345,955 - -0.1
345,986 + +0.3
346,042 + +0.5
346,042 + -1.7
346,042 + +3.5
346,042 + +0.2
346,042 + -0.5
346,042 + -0.5
346,043 - +0.2
346,043 - -0.8
346,043 - +0.5
346,115 - -0.1
346,116 + +4.4
346,116 + -1.5
346,169 + +0.6
346,170 - +0.7
346,170 - +0.6
346,406 + PS417_01560 0.42 -1.6
346,407 - PS417_01560 0.42 +1.0
346,446 + PS417_01560 0.48 +1.3
346,446 + PS417_01560 0.48 +0.2
346,524 + PS417_01560 0.62 +0.7
346,822 - PS417_01565 0.11 +0.2

Or see this region's nucleotide sequence