Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14855 and PS417_14860 are separated by 218 nucleotides PS417_14860 and PS417_14865 are separated by 28 nucleotides PS417_14865 and PS417_14870 are separated by 90 nucleotides PS417_14870 and PS417_14875 overlap by 4 nucleotides
PS417_14855: PS417_14855 - gluconolactonase, at 3,245,464 to 3,246,561
_14855
PS417_14860: PS417_14860 - hypothetical protein, at 3,246,780 to 3,247,211
_14860
PS417_14865: PS417_14865 - GntR family transcriptional regulator, at 3,247,240 to 3,248,565
_14865
PS417_14870: PS417_14870 - transporter, at 3,248,656 to 3,249,546
_14870
PS417_14875: PS417_14875 - polyisoprenoid-binding protein, at 3,249,543 to 3,250,097
_14875
Position (kb)
3247
3248
3249 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3246.581 kb on - strand at 3246.586 kb on + strand at 3246.674 kb on + strand at 3246.675 kb on - strand at 3246.829 kb on - strand, within PS417_14860 at 3247.201 kb on - strand at 3247.201 kb on - strand at 3247.207 kb on - strand at 3247.207 kb on - strand at 3247.374 kb on + strand, within PS417_14865 at 3247.713 kb on - strand, within PS417_14865 at 3247.713 kb on - strand, within PS417_14865 at 3247.713 kb on - strand, within PS417_14865 at 3247.713 kb on - strand, within PS417_14865 at 3247.713 kb on - strand, within PS417_14865 at 3247.889 kb on + strand, within PS417_14865 at 3247.889 kb on + strand, within PS417_14865 at 3247.889 kb on + strand, within PS417_14865 at 3247.889 kb on + strand, within PS417_14865 at 3248.573 kb on + strand at 3248.573 kb on + strand at 3248.573 kb on + strand at 3248.574 kb on - strand at 3248.574 kb on - strand at 3248.574 kb on - strand at 3248.599 kb on + strand at 3248.665 kb on - strand at 3248.976 kb on - strand, within PS417_14870 at 3249.068 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.328 kb on + strand, within PS417_14870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,246,581 - +4.9 3,246,586 + +0.4 3,246,674 + -1.7 3,246,675 - -0.1 3,246,829 - PS417_14860 0.11 +1.2 3,247,201 - -0.3 3,247,201 - +0.5 3,247,207 - +0.0 3,247,207 - -0.0 3,247,374 + PS417_14865 0.10 +0.3 3,247,713 - PS417_14865 0.36 +0.2 3,247,713 - PS417_14865 0.36 -0.8 3,247,713 - PS417_14865 0.36 +0.5 3,247,713 - PS417_14865 0.36 +0.7 3,247,713 - PS417_14865 0.36 +0.1 3,247,889 + PS417_14865 0.49 +0.3 3,247,889 + PS417_14865 0.49 +1.5 3,247,889 + PS417_14865 0.49 -0.1 3,247,889 + PS417_14865 0.49 +0.8 3,248,573 + +0.7 3,248,573 + +0.0 3,248,573 + -1.3 3,248,574 - +0.2 3,248,574 - -0.7 3,248,574 - +0.1 3,248,599 + -0.4 3,248,665 - +0.5 3,248,976 - PS417_14870 0.36 +0.3 3,249,068 + PS417_14870 0.46 +0.3 3,249,072 + PS417_14870 0.47 -0.3 3,249,072 + PS417_14870 0.47 -0.1 3,249,072 + PS417_14870 0.47 -1.3 3,249,328 + PS417_14870 0.75 -1.0
Or see this region's nucleotide sequence