Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14650 and PS417_14655 are separated by 127 nucleotides PS417_14655 and PS417_14660 are separated by 60 nucleotides PS417_14660 and PS417_14665 are separated by 111 nucleotides PS417_14665 and PS417_14670 are separated by 29 nucleotides
PS417_14650: PS417_14650 - chemotaxis protein, at 3,200,581 to 3,202,146
_14650
PS417_14655: PS417_14655 - lysine transporter LysE, at 3,202,274 to 3,202,894
_14655
PS417_14660: PS417_14660 - methyltransferase, at 3,202,955 to 3,203,611
_14660
PS417_14665: PS417_14665 - TetR family transcriptional regulator, at 3,203,723 to 3,204,355
_14665
PS417_14670: PS417_14670 - hypothetical protein, at 3,204,385 to 3,204,657
_14670
Position (kb)
3202
3203
3204 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3202.058 kb on + strand at 3202.059 kb on - strand at 3202.235 kb on - strand at 3202.505 kb on + strand, within PS417_14655 at 3203.040 kb on + strand, within PS417_14660 at 3203.041 kb on - strand, within PS417_14660 at 3203.053 kb on - strand at 3203.124 kb on + strand, within PS417_14660 at 3203.159 kb on + strand at 3203.406 kb on + strand, within PS417_14660 at 3203.406 kb on + strand, within PS417_14660 at 3203.407 kb on - strand, within PS417_14660 at 3203.464 kb on - strand, within PS417_14660 at 3203.475 kb on + strand, within PS417_14660 at 3203.562 kb on + strand at 3203.562 kb on + strand at 3203.563 kb on - strand at 3203.611 kb on + strand at 3203.612 kb on - strand at 3203.722 kb on + strand at 3203.746 kb on + strand at 3204.317 kb on - strand at 3204.329 kb on + strand at 3204.329 kb on + strand at 3204.330 kb on - strand at 3204.432 kb on - strand, within PS417_14670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,202,058 + -1.9 3,202,059 - +1.1 3,202,235 - -2.6 3,202,505 + PS417_14655 0.37 +0.5 3,203,040 + PS417_14660 0.13 +0.8 3,203,041 - PS417_14660 0.13 +0.3 3,203,053 - +1.8 3,203,124 + PS417_14660 0.26 +0.7 3,203,159 + -0.8 3,203,406 + PS417_14660 0.69 +2.2 3,203,406 + PS417_14660 0.69 +0.3 3,203,407 - PS417_14660 0.69 +1.1 3,203,464 - PS417_14660 0.77 -0.2 3,203,475 + PS417_14660 0.79 +0.5 3,203,562 + -0.6 3,203,562 + -0.2 3,203,563 - -0.0 3,203,611 + -0.9 3,203,612 - -0.1 3,203,722 + +0.4 3,203,746 + -0.4 3,204,317 - +2.4 3,204,329 + -0.0 3,204,329 + -0.9 3,204,330 - +0.7 3,204,432 - PS417_14670 0.17 +3.0
Or see this region's nucleotide sequence