Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14520 and PS417_14525 overlap by 4 nucleotides PS417_14525 and PS417_14530 are separated by 178 nucleotides PS417_14530 and PS417_14535 are separated by 138 nucleotides
PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908
_14520
PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564
_14525
PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504
_14530
PS417_14535: PS417_14535 - alpha/beta hydrolase, at 3,177,643 to 3,178,677
_14535
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3176.376 kb on + strand, within PS417_14525 at 3176.577 kb on + strand at 3176.578 kb on - strand at 3176.626 kb on + strand at 3176.827 kb on - strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.275 kb on - strand, within PS417_14530 at 3177.516 kb on + strand at 3177.545 kb on + strand at 3177.546 kb on - strand at 3177.556 kb on + strand at 3177.587 kb on + strand at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,176,376 + PS417_14525 0.71 +0.3 3,176,577 + -0.5 3,176,578 - -0.3 3,176,626 + -2.1 3,176,827 - PS417_14530 0.11 +0.4 3,177,274 + PS417_14530 0.70 -0.8 3,177,274 + PS417_14530 0.70 -1.8 3,177,275 - PS417_14530 0.70 +2.8 3,177,516 + -0.5 3,177,545 + -1.3 3,177,546 - -0.5 3,177,556 + -1.0 3,177,587 + -0.2 3,177,902 - PS417_14535 0.25 -1.5 3,177,902 - PS417_14535 0.25 -1.4 3,177,902 - PS417_14535 0.25 -0.1 3,177,902 - PS417_14535 0.25 +0.0
Or see this region's nucleotide sequence