Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14310 and PS417_14315 are separated by 61 nucleotides PS417_14315 and PS417_14320 are separated by 3 nucleotides
PS417_14310: PS417_14310 - dihydrodipicolinate synthetase, at 3,132,187 to 3,133,134
_14310
PS417_14315: PS417_14315 - 2,5-dioxovalerate dehydrogenase, at 3,133,196 to 3,134,767
_14315
PS417_14320: PS417_14320 - FAD-dependent oxidoreductase, at 3,134,771 to 3,136,009
_14320
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3132.319 kb on + strand, within PS417_14310 at 3132.592 kb on + strand, within PS417_14310 at 3132.592 kb on + strand, within PS417_14310 at 3132.605 kb on - strand, within PS417_14310 at 3132.776 kb on + strand, within PS417_14310 at 3132.973 kb on + strand, within PS417_14310 at 3132.974 kb on - strand, within PS417_14310 at 3132.976 kb on + strand, within PS417_14310 at 3132.976 kb on + strand, within PS417_14310 at 3132.976 kb on + strand, within PS417_14310 at 3132.977 kb on - strand, within PS417_14310 at 3132.977 kb on - strand, within PS417_14310 at 3133.013 kb on - strand, within PS417_14310 at 3133.182 kb on - strand at 3133.545 kb on + strand, within PS417_14315 at 3134.012 kb on + strand, within PS417_14315 at 3134.013 kb on - strand, within PS417_14315 at 3134.013 kb on - strand, within PS417_14315 at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.791 kb on - strand at 3134.898 kb on - strand, within PS417_14320 at 3134.898 kb on - strand, within PS417_14320 at 3135.077 kb on + strand, within PS417_14320 at 3135.171 kb on + strand, within PS417_14320 at 3135.172 kb on - strand at 3135.372 kb on - strand, within PS417_14320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,132,319 + PS417_14310 0.14 -0.9 3,132,592 + PS417_14310 0.43 +0.3 3,132,592 + PS417_14310 0.43 -0.8 3,132,605 - PS417_14310 0.44 +1.0 3,132,776 + PS417_14310 0.62 -2.3 3,132,973 + PS417_14310 0.83 -2.3 3,132,974 - PS417_14310 0.83 +3.5 3,132,976 + PS417_14310 0.83 -1.5 3,132,976 + PS417_14310 0.83 -2.2 3,132,976 + PS417_14310 0.83 -1.5 3,132,977 - PS417_14310 0.83 +0.9 3,132,977 - PS417_14310 0.83 -1.5 3,133,013 - PS417_14310 0.87 +0.5 3,133,182 - +0.4 3,133,545 + PS417_14315 0.22 -1.3 3,134,012 + PS417_14315 0.52 -0.8 3,134,013 - PS417_14315 0.52 -0.9 3,134,013 - PS417_14315 0.52 -0.0 3,134,620 + +0.8 3,134,620 + -0.3 3,134,620 + +1.2 3,134,620 + +2.0 3,134,791 - +0.0 3,134,898 - PS417_14320 0.10 -0.1 3,134,898 - PS417_14320 0.10 +1.1 3,135,077 + PS417_14320 0.25 -0.8 3,135,171 + PS417_14320 0.32 +0.1 3,135,172 - +1.8 3,135,372 - PS417_14320 0.49 +0.1
Or see this region's nucleotide sequence