Strain Fitness in Pseudomonas simiae WCS417 around PS417_13575

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_13565 and PS417_13575 are separated by 585 nucleotidesPS417_13575 and PS417_13580 overlap by 39 nucleotidesPS417_13580 and PS417_13585 are separated by 143 nucleotides PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579 _13565 PS417_13575: PS417_13575 - succinylglutamate desuccinylase, at 2,990,165 to 2,991,163 _13575 PS417_13580: PS417_13580 - AraC family transcriptional regulator, at 2,991,125 to 2,991,982 _13580 PS417_13585: PS417_13585 - alpha/beta hydrolase, at 2,992,126 to 2,992,926 _13585 Position (kb) 2990 2991 2992Strain fitness (log2 ratio) -2 -1 0 1at 2989.304 kb on + strand, within PS417_13565at 2989.675 kb on - strandat 2989.999 kb on + strandat 2990.142 kb on - strandat 2990.149 kb on + strandat 2990.310 kb on + strand, within PS417_13575at 2990.374 kb on + strand, within PS417_13575at 2990.609 kb on + strandat 2990.610 kb on - strand, within PS417_13575at 2990.903 kb on - strand, within PS417_13575at 2991.138 kb on - strandat 2991.331 kb on - strand, within PS417_13580at 2991.868 kb on - strand, within PS417_13580at 2991.994 kb on + strandat 2992.001 kb on + strandat 2992.001 kb on + strandat 2992.002 kb on - strandat 2992.029 kb on - strandat 2992.056 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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2,989,304 + PS417_13565 0.72 -0.2
2,989,675 - -0.9
2,989,999 + -1.3
2,990,142 - +0.1
2,990,149 + -1.9
2,990,310 + PS417_13575 0.15 +0.1
2,990,374 + PS417_13575 0.21 -1.1
2,990,609 + -0.3
2,990,610 - PS417_13575 0.45 -1.3
2,990,903 - PS417_13575 0.74 -1.3
2,991,138 - +0.0
2,991,331 - PS417_13580 0.24 -0.4
2,991,868 - PS417_13580 0.87 +1.0
2,991,994 + +0.9
2,992,001 + +0.9
2,992,001 + +0.9
2,992,002 - -0.1
2,992,029 - +1.2
2,992,056 + -1.8

Or see this region's nucleotide sequence